張曉東

主要教育和工作經歷：

1991-1995 武漢大學生命科學學院學士

1995-1998 武漢大學生命科學學院碩士

1998-2000 中國醫學科學院基礎醫學研究所博士

2004-2005 Georgetown University 博士后

2000-2004 中國醫學科學院基礎醫學研究所助理研究員，副研究員

2005-2008 Case Western Reserve University 研究助理

2008-至今武漢大學生命科學學院教授

主要任職、獲獎及榮譽：

生命科學學院儀器設備共享中心主任

2011年獲教育部“新世紀優秀人才計劃”

中華預防醫學會科學技術獎評審委員會委員

湖北省腸病中心學術委員會委員

湖北省實驗動物專家委員會委員。

目前主要研究領域和興趣：

本實驗室以體細胞、斑馬魚、小鼠和大鼠基因敲除/敲入技術為主要研究方法，從分子水平探討腫瘤的發病機理, 研究相關基因作用途徑和尋找理想的分子靶標，為腫瘤的早期診斷、治療和預后提供理論依據。

主持課題項目：

1. 項目首席科學家：國家科技支撐計劃（2014BAI02B00），2014.07-2017.06

2. 課題組長：國家重大科學研究計劃（2011CB944404），2011.01-2015.12

3. 項目負責人：國家自然科學基金面上項目（30971499），2010.01-2012.12

4. 項目負責人：國家自然科學基金面上項目（81270306），2013.01-2016.12

5. 項目負責人：國家自然科學基金面上項目（81470375），2015.01-2018.12

6. 課題骨幹：國家科技支撐計劃（2012BAI39B03），2012.01-2014.12

教學情況：

分子生物學，本科生課程，3學分

實驗室研究技術訓練，研究生課程，2學分

細胞生物學研究前沿，研究生課程，2學分

代表論文和著作

X.F. Zhang, K.K. Li, L. Gao, S.Z. Li, K. Chen, J.B. Zhang, D. Wang, R.F. Tu, J.X. Zhang, K.X. Tao, G. Wang,X.D. Zhang,miR-191 promotes tumorigenesis of human colorectal cancer through targeting C/EBPbeta, Oncotarget, 6 (2015) 4144-4158.

Z.Y. Zhang, S.Z. Li, H.H. Zhang, Q.R. Wu, J. Gong, T. Liang, L. Gao, N.N. Xing, W.B. Liu, R.L. Du,X.D. Zhang,Stabilization of ATF5 by TAK1-NLK Critically Regulates the IL-1beta-stimulated C/EBP Signaling Pathway, Mol Cell Biol, (2014).

X.F. Zhang, R. Zhang, L. Huang, P.X. Wang, Y. Zhang, D.S. Jiang, L.H. Zhu, S. Tian,X.D. Zhang,H. Li, TRAF1 is a key mediator for hepatic ischemia/reperfusion injury, Cell death & disease, 5 (2014) e1467.

H.H. Zhang, S.Z. Li, Z.Y. Zhang, X.M. Hu, P.N. Hou, L. Gao, R.L. Du,X.D. Zhang,Nemo-like kinase is critical for p53 stabilization and function in response to DNA damage, Cell death and differentiation, 21 (2014) 1656-1663.

L. Yan, W. Liu, H. Zhang, C. Liu, Y. Shang, Y. Ye,X. Zhang,W. Li, Ube2g2-gp78-mediated HERP polyubiquitylation is involved in ER stress recovery, Journal of cell science, 127 (2014) 1417-1427.

S.Z. Li, H.H. Zhang, J.B. Liang, Y. Song, B.X. Jin, N.N. Xing, G.C. Fan, R.L. Du,X.D. Zhang,Nemo-like kinase (NLK) negatively regulates NF-kappa B activity through disrupting the interaction of TAK1 with IKKbeta, Biochimica et biophysica acta, (2014).

S.-Z. Li, Y. Song, H.-H. Zhang, B.-X. Jin, Y. Liu, W.-B. Liu,X.-D. Zhang,R.-L. Du, UbcH10 overexpression increases carcinogenesis and blocks ALLN susceptibility in colorectal cancer, Sci. Rep., 4 (2014).

X.A. Wang, R. Zhang, Z.G. She, X.F. Zhang, D.S. Jiang, T. Wang, L. Gao, W. Deng, S.M. Zhang, L.H. Zhu, S. Guo, K. Chen,X.D. Zhang,D.P. Liu, H. Li, Interferon regulatory factor 3 constrains IKKbeta/NF-kappaB signaling to alleviate hepatic steatosis and insulin resistance, Hepatology, (2013).

L. Wang, Y. Lu, X. Zhang, Y. Zhang, D. Jiang, X. Dong, S. Deng, L. Yang, Y. Guan, L. Zhu, Y. Zhou,X. Zhang,H. Li, Mindin is a critical mediator of ischemic brain injury in an experimental stroke model, Experimental neurology, 247 (2013) 506-516.

S.Z. Li, H.H. Zhang, J.N. Zhang, Z.Y. Zhang, X.F. Zhang,X.D. Zhang,R.L. Du, ALLN hinders HCT116 tumor growth through Bax-dependent apoptosis, Biochemical and biophysical research communications, 437 (2013) 325-330.

D.S. Jiang, X.F. Zhang, L. Gao, J. Zong, H. Zhou, Y. Liu, Y. Zhang, Z.Y. Bian, L.H. Zhu, G.C. Fan,X.D. Zhang, H. Li, Signal Regulatory Protein-alpha Protects Against Cardiac Hypertrophy Via the Disruption of Toll-Like Receptor 4 Signaling, Hypertension, (2013).

Z. Huang, S. Li, W. Song, X. Li, Q. Li, Z. Zhang, Y. Han,X. Zhang,S. Miao, R. Du, L. Wang, Lysine-specific demethylase 1 (LSD1/KDM1A) contributes to colorectal tumorigenesis via activation of the Wnt/Beta-catenin pathway by down-regulating Dickkopf-1 (DKK1), PloS one, 8 (2013) e70077.

K. Chen, L. Gao, Y. Liu, Y. Zhang, D.-S. Jiang, X. Wei, X. Zhu, R. Zhang, Y. Chen, Q. Yang, N. Kioka,X.-D. Zhang,H. Li, Vinexin-β protects against cardiac hypertrophy by blocking the Akt-dependent signalling pathway, Basic research in cardiology, 108 (2013) 1-14.

Y. Liu, S. Li, H. Zhang, Z. Wan,X. Zhang,R. Du, A one-step cloning method for the construction of somatic cell gene targeting vectors: application to production of human knockout cell lines, BMC biotechnology, 12 (2012) 71.

P. Zhang, Y. Zhao, X. Zhu, D. Sedwick,X. Zhang,Z. Wang, Cross-talk between phospho-STAT3 and PLCgamma1 plays a critical role in colorectal tumorigenesis, Molecular cancer research : MCR, 9 (2011) 1418-1428.

R. Du, S. Li,X. Zhang,A modified plasmid vector pCMV-3Tag-LIC for rapid, reliable, ligation-independent cloning of polymerase chain reaction products, Analytical biochemistry, 408 (2011) 357-359.

P. Wang, F. Zou,X. Zhang,H. Li, A. Dulak, R.J. Tomko, Jr., J.S. Lazo, Z. Wang, L. Zhang, J. Yu, microRNA-21 negatively regulates Cdc25A and cell cycle progression in colon cancer cells, Cancer research, 69 (2009) 8157-8165.

X. Zhang,C. Guo, Y. Chen, H.P. Shulha, M.P. Schnetz, T. LaFramboise, C.F. Bartels, S. Markowitz, Z. Weng, P.C. Scacheri, Z. Wang, Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies, Nature methods, 5 (2008) 163-165.

C. Guo,X. Zhang,S.P. Fink, P. Platzer, K. Wilson, J.K. Willson, Z. Wang, S.D. Markowitz, Ugene, a newly identified protein that is commonly overexpressed in cancer and binds uracil DNA glycosylase, Cancer research, 68 (2008) 6118-6126.

X. Zhang,A. Guo, J. Yu, A. Possemato, Y. Chen, W. Zheng, R.D. Polakiewicz, K.W. Kinzler, B. Vogelstein, V.E. Velculescu, Z.J. Wang, Identification of STAT3 as a substrate of receptor protein tyrosine phosphatase T, Proceedings of the National Academy of Sciences of the United States of America, 104 (2007) 4060-4064.