黃健

學習簡歷

1986-1990年 武漢大學生命科學學院 生物化學專業　獲學士學位。

1993-1996年 武漢大學生命科學學院 生物化學專業　獲碩士學位。

1996-1999年 武漢大學生命科學學院 微生物學專業　獲博士學位。

研究工作簡歷

講　師　1999-2001年　武漢大學生命科學學院。

助　研　1999-2000年　香港理工大學應用生物及化學學系。

博士后　2001-2003年　美國肯塔基大學生物化學學系。

副教授　2001-2009年　武漢大學生命科學學院。

教　授　2009-現　在　武漢大學生命科學學院。

1.主講《生物化學技術原理極其應用》(本科生)。

2.主講《專業文獻研讀》（本科生）。

3.主講《神經生物學》(研究生)。

研究方向：神經生物化學和分子生物學。

教育科研

主要研究領域與興趣。

激素防治Alzheimer病的機制研究。

新型ERβ選擇性雌激素受體調節劑的篩選和研究。

主持科研項目

教育部留學回國人員科研啟動基金。

國家自然科學基金面上項目。

國家自然科學基金二個基地項目。

國家自然科學基金面上項目。

湖北省自然科學基金（2006ABA219）。

湖北省科技攻關計劃項目（2006AA301B31）。

湖北省高等學校教學研究項目（20062022）。

湖北省自然科學基金（2007ABA115）。

教材《生物化學技術原理極其應用》第四版，副主編，2008年，科學出版社。

代表性論文

1. Zhimin Xiao, Can Huang, Jun Wu, Li Sun, Wei Hao, Lai K. Leung, Jian Huang*. The neuroprotective effects of ipriflavone against H2O2 and amyloid beta induced toxicity on human neuroblastoma SH-SY5Y cells. Eur J Pharmacol. (2013)

2. Caihua Wang, Changhao Li, Haibing Zhou and Jian Huang*. High-Throughput Screening Assays for Estrogen Receptor by Using Coumestrol, a Natural Fluorescence Compound. J Biomol Screen. (2013).

3. Wenjuan Tan, Tsz Yan Wong, Yanfei Wang, Jian Huang, Lai K. Leung*. CYP19 expression is induced by 2,3,7,8-tetrachloro-dibenzo-para-dioxin in human glioma cells. Mol Cell Endocrinol. 15;375(1-2):106-12. (2013).

4. Nelson L. S. Chan, Jinzhen Guo, Tianyi Zhang, Guogen Mao, Caixia Hou, Fenghua Yuan, Jian Huang, Yanbin Zhang, Jianxin Wu, Liya Gu, and Guo-Min Li*. Coordinated processing of 3' slipped (CAG)n/(CTG)n hairpins by DNA polymerases β and δ preferentially induces repeat expansions. J Biol Chem. 288(21): 15015–15022, (2013).

5. Xinlong Zhang, Ziqing Zuo, Juan Tang, Kai Wang, Caihua Wang, Weiyan Chen, Changhao Li, Wen Xu, Xiaolin Xiong, Kangxiang Yuntai, Jian Huang, Xiaoli Lan, Hai-Bing Zhou*. Design, synthesis and biological evaluation of novel estrogen-derived steroid metal complexes. Bioorg Med Chem Lett. 1;23(13):3793-7. (2013).

6. Feng Li, Guogen Mao, Dan Tong, Jian Huang, Liya Gu, Wei Yang and Guo-Min Li*. The Histone Mark H3K36me3 Regulates Human DNA Mismatch Repair through Its Interaction with MutSα. Cell. 153(3):590-600. (2013).

7. Yangfan Zheng, Caihua Wang, Changhao Li, Jinxia Qiao, Feng Zhang, Minjian Huang, Wenming Ren, Chune Dong, Jian Huang* and Hai-Bing Zhou*. Discovery of novel SERMs with a ferrocenyl entity based on the oxabicyclo[2.2.1]heptene scaffold and evaluation of their antiproliferative effects in breast cancer cells. Org. Biomol. Chem., 10: 9689. (2012).

8. Nelson L. S. Chan, Caixia Hou, Tianyi Zhang, Fenghua Yuan, Amrita Machwe, Jian Huang, David K. Orren,Liya Gu, and Guo-Min Li?. The Werner syndrome protein promotes CAG/CTG repeat stability by resolving large (CAG)(n)/(CTG)(n) hairpins. J Biol Chem. 287(36):30151-6. (2012).

9. Hsiang-Tsui Wang, YuHu, Dan Tong, Jian Huang, Liya Gu, Xue-Ru Wu, Fung-Lung Chung, Guo-Min Li,and Moon-shong Tang?. Effect of Carcinogenic Acrolein on DNA Repair and Mutagenic Susceptibility. J Biol Chem. 287(15): 12379–86. (2012).

1徠0. Tianyi Zhang, Jian Huang*, Liya Gu, Guo-Min Li?. In vitro repair of DNA hairpins containing various numbers of CAG/CTG trinucleotide repeats. DNA Repair, 11: 201–209. (2012).

11. Peng Yuan, Kaiwei Liang, Buyong Ma, Nan Zheng, Ruth Nussinov, and Jian Huang*. Multiple-Targeting and Conformational Selection in the Estrogen Receptor: Computation and Experiment. Chem Biol Drug Des, 78: 137–149. (2011).

12. Caixia Hou, Tianyi Zhang, Lei Tian, Jian Huang, Liya Gu, Guo-Min Li*. The Role of XPG in Processing (CAG)n/(CTG)n DNA Hairpins. Cell & Bioscience, 1:11-17. (2011).

13. Kaiwei Liang, Liuqing Yang, Chen Yin, Zhimin Xiao, Junjian Zhang, Yumin Liu and Jian Huang*, Estrogen stimulates degradation of {beta}-amyloid peptide by upregulating neprilysin. J Biol Chem. 285(2):935-42. (2010). doi:10.1074/jbc.M109.051664

14. Zhi-Min Xiao; Li Sun; Yu-Min Liu; Jun-Jian Zhang; Jian Huang*, Estrogen Regulation of the Neprilysin Gene Through A Hormone-Responsive Element. Journal of Molecular Neuroscience, 39:22-6. (2009). doi:10.1007/s12031-008-9168-1

15. Kaiwei Liang, Liuqing Yang, Zhimin Xiao and Jian Huang*, A Bipartite Recombinant Yeast System for the Identification of Subtype-Selective Estrogen Receptor Ligands. Molecular Biotechnology, 41:53–62, (2009). doi:10.1007/s12033-008-9097-9

16. Li-Juan Deng, Yan Xu, Jian Huang*, Developing a double-antigen sandwich ELISA for effective detection of human hepatitis B core antibody. Comp Immunnol Microb, 31: 515-26. (2008).doi:10.1016/j.cimid.2007.09.001

17. Guogen Mao, Xiaoyu Pan, Bei-Bei Zhu, Yanbin Zhang, Fenghua Yuan, Jian Huang, Mark A. Lovell, Maxwell P. Lee, William R. Markesbery, and Guo-Min Li, Liya Gu. Identification and characterization of OGG1 mutations in patients with Alzheimer’s disease. Nucleic Acids Research, 35(8):2759-66. (2007). doi:10.1093/nar/gkm189

18. Huang, J., Guan, H., Booze, R. M., Eckman, C. B., Hersh, L.B. Estrogen regulates neprilysin activity in rat brain. Neurosci Lett. 367(1):85-7. (2004)．doi:10.1016/j.neulet.2004.05.085

19. Deming Gou, Jun Wang, Li Gao, Yan Sun, Xiao Peng, Jian Huang, Wenxin Li. Identification and functional analysis of a novel Human KRAB/C2H2 zinc finger gene ZNF300. Biochimica et biophysica acta, 2004 ,1676 (3):203-209. doi:10.1016/j.bbaexp.2003.11.011

20. Huang, J., Gou, D. M., Zhen, C. Y., Jiang, D. H., Mao, X., Li, W. X. Protection of xenogeneic cells from human complement-mediated lysis by expression of human DAF, CD 59 and MCP. FEMS Immunology and Medical Microbiology, 31(3):203-9. (2001).

21. Gou DM; Sun Y; Gao L; Chow LM; Huang J; Feng YD; Jiang DH; Li WX. Cloning and characterization of a novel Kruppel-like zinc finger gene, ZNF268, expressed in early human embryo. Biochimica et biophysica acta, 2001 ,1518(3):306-10.