| 角色 | 演員 | 備註 |
| Toxic Avenger/Noxious Offender/Man getting a B.J. from Chester | David Mattey | |
| Toxie (voice) | Clyde Lewis | |
| Sarah/Claire | Heidi Sjursen | |
| Sgt. Kabukiman/Evil Kabukiman | Paul Kyrmse | |
| Chester/Lardass | Joe Fleishaker | |
| Tito | Michael Budinger | |
| Sweetie Honey | Lisa Terezakis | |
| Sgt. Kazinski | Dan Snow | |
| Ms. Weiner | Debbie Rochon | |
| Pompey | Barry Brisco | |
| Mayor Goldberg (death by crucifix through mouth) | 羅恩·傑里米（Ron Jeremy） | |
| Sarah's Gynecologist (as Kinky Finkelstein) | 科里·費爾德曼（Corey Feldman） | |
| Amortville Police Chief | Rick Collins | |
| Evil Melvin | Mark Torgl | |
| Tex Diaper (Disembowelment Death) | Trent Haaga | |
| Lucifer (Dragging Redneck #1) (Lynching Death) | Mitch Cohen | |
| Dex Diaper (death by suffocation with own diaper) | Caleb Emerson | |
| Abortion Counselor | Lisa Gaye | |
| Rex Diaper | Yaniv Sharon | |
| Abortion Doctor (Speculum Death) | Bill Weeden | |
| Tromaville Chief Newman | Jon Avner | |
| Doctor Flem Hocking (Car Crash Death) | 詹姆斯·古恩（James Gunn） | |
| Bikini Anchor Samantha Chambers | Carla Pivonski | |
| Beautiful Interpretative Dancer | 蒂凡妮·謝皮斯（Tiffany Shepis） | |
| God | Hank The Angry Dru.. | |
| Mayor Goldberg's Press Secretary | Al Goldstein | |
| Award Presenter (DVD) | Taylor Mead | |
| Weekly World News Reporter (DVD) | Dick Kulpa | |
| Lesbian Art Student | Tromelissa Saytar | |
| Festive Wheelchair-Bound Special Student | Joseph Floccari | |
| Jason Diaz (Action News Anchor #1) (as Foofy Applebaum) | Jason Sklar | |
| Lucky Retard Stud | Chris Bagnall | |
| Jason Gonzales (Action News Anchor #2) (as Skippy Applebaum) | Randy Sklar | |
| Good Lemmy/Bad Lemmy (as Lemmy Kilmeister) | 萊米（Lemmy） | |
| Herself (Facelift Death) | 朱莉·斯翠（Julie Strain） | |
| Himself (Breast Smoosh Death) | 凱文·伊斯特曼（Kevin Eastman） | |
| Dolphinman (death by severe ass beating) | Olivier Tendon | |
| Master-Bater (death by severe ass beating) | Jarred Alterman | |
| Mad Cowboy (death by severe ass beating) | Anthony Cantanese | |
| The Vibrator (death by severe ass beating) | Lauren Heather McM.. | |
| President of the United States | 休·海夫納（Hugh M. Hefner） | |
| Special Student/Plastic Surgery Patient | Doug Sakmann | |
| Bar Mitzvah Guy | Devin Hamilton | |
| St. Peter | Haig Demarjian | |
| Diaper Mafia Temptress (with Beautiful Breasts) | Nicole Howe | |
| Diaper Mafia Temptress (with Beautiful Breasts) | Bella Compagna | |
| Prologue/Epilogue Narrator (voice) (as Peter Parker) | 斯坦·李（Stan Lee） | |
| Beautiful Young Boy | 伊萊·羅斯（Eli Roth） | |
| Duel Gyno | Purple Pam Shelby | |
| Death by Pencil in Eye | Andy Braunstein | |
| Grotto Penis Boy | Andrew Kirby | |
| Death by Overhead Toxie Hurl | Marcia Lynn | |
| Male Nurse | Don May Jr. | |
| Other Diaper Mafia Member | R.H. Bear | |
| Fudgie | Geoff Mosher | |
| Other Diaper Mafia Member | Rich Preach | |
| Other Diaper Mafia Member | Antonio Tomahawk | |
| Bomb Voice (voice) | Christina Hibbs | |
| Man Hit by Car | Will Keenan | |
| News Producer | Mike Butler | |
| Josh Levitsky's Super-Hero Pal/Radio News Announcer/Tromaville Citizen | Gabriel Friedman | |
| Josh Levitsky's Super-Hero Pal/Homosexual #2 | Sean McGrath | |
| Reporter #1 (Death by Strangulation with Severed Arms) | Heather Litteer | |
| Reporter 3 | Eric Alan | |
| Sara's Dream Man | Joseph Anthony | |
| Dr. Hocking's Nurse | Melissa Bacelar | |
| Protester at clinic | Gerald Blakey | |
| Glamorous Gyno-American | Brandi Brandt | |
| Woman who Falls from High Rock | Alisa Christensen | |
| Self | Lenora Claire | |
| Nazi with Eye Patch/Janitor on Fire | Timothy Considine | |
| Internet Contest Winner (Death by Pole in Head) | Erik Dage | |
| Glamorous Gyno-American | Neriah Davis | |
| Glamorous Gyno-American | Devin DeVasquez | |
| Citizen | Rev Douglas | |
| Wet Lesbian/Toples Deaf Translator | Terri Firmer | |
| Artist at Tromadu (Eyes poked out) | Tom Fulp | |
| Reporter | Elizabeth Johnson | |
| Tromaville/Amortville Citizen | Charlotte Kaufman | |
| Wet Lesbian (as Roxanne Michaels) | Roxanne Michaels | |
| Nurse | Crystal McCloud | |
| Dragging Redneck #2 (as Gregory John Nolasco) | Greg Nolasco | |
| Angelique, Kinky Finklestein's Assistant | Celeste Octavia | |
| Special student victim | James Ojala | |
| Special Student | Tobias Suhm | |
| Old Woman (Death by Car Tire Squashing Head) | Doris Troncillito | |
| Gay 70's porno dimension film crew member | Tony Tuff | |
| Reporter (as Maureen Layer) | Eric Waddell | |
| Julie Strain Double | Kathleen Wirt | |
| Bystander (uncredited) | Mark C. Adams | |
| Tromaville Citizen (uncredited) | Brendan Flynt | |
| Police Officer (uncredited) | Jason Horton | |
| Tromaville Citizen (uncredited) | Adam Jahnke | |
| Man in Public Service Announcement (uncredited) | 洛伊德考夫曼（Lloyd Kaufman） | |
| Tromaville Citizen (uncredited) | Brian Macaya | |
