葉明亮

葉明亮 研究員 博士生導師 中科院大連化學物理研究所 生物技術部

葉明亮，以色譜分離與質譜分析為立足點，針對蛋白質組等複雜生物樣品的高效分離與高靈敏檢測，開展蛋白質組分離分析新技術和新方法的研究，注重分析化學與生物學、數學、生物信息學等學科的交叉。在蛋白質翻譯后修飾的高靈敏、規模化分析方面建立了多項原創技術，在Nat. Methods , Nat. Chem. Biol., Nat. Protoc., Angew. Chem. Int. Ed. 等SCI期刊上發表責任作者（第一作者和通訊作者）論文70餘篇，他引2000餘次（所有SCI論文170餘篇，他引4400餘次）。曾獲中科院院長獎學金特別獎（2001年），遼寧省自然科學獎兩次（2004年排名第4；2011年排名第3；），國家自然科學二等獎一次(2012年排名第3)。先後主持基金委傑出青年基金、國家重點研發計劃重點專項、基金委中加健康合作項目、科技部創新方法項目、國家重大科學研究計劃課題等項目。

葉明亮博士主要研究方向為蛋白質組新技術新方法的研究，主要包括：

l 複雜生物樣品預處理技術和方法

基於納米材料的富集技術和方法、蛋白質組樣品預處理的集成化技術、同位素標記技術

l 高效分離柱技術與多維分離分析系統

高效整體分離柱技術；多維分離分析方法；質譜數據處理方法

l 蛋白質組修飾譜規模化分析技術和方法

蛋白質磷酸化、糖基化等翻譯后修飾的分離、規模化鑒定新方法及其應用

l 應用研究

血液凈化材料、生物分離材料、東北特色資源研究與開發

REPRESENTATIVE PUBLICATIONS

1）Yangyang Bian, Lei Li, Mingming Dong, Xuguang Liu, Tomonori Kaneko, Kai Cheng, Huadong Liu, Courtney Voss, Xuan Cao, Yan Wang, David Litchfield, Mingliang Ye*, Shawn S.-C. Li*, and Hanfa Zou*. Ultra-deep tyrosine phosphoproteomics enabled by a phosphotyrosine superbinder, Nature Chemical Biology, 2016, in press.

2） Ye, Mingliang*;Pan, Yanbo; Cheng, Kai; Zou, Hanfa*. Protein Digestion Priority is Independent of Protein Abundances. Nature Methods, 2014, 11: 220-222.

3）Pan, Yanbo; Ye, Mingliang*;Zhao, Liang; Cheng, Kai; Dong, Mingming; Song, Chunxia; Qin,Hongqiang; Wang, Fangjun; Zou, Hanfa*. N-Terminal Labeling of Peptides by Trypsin-Catalyzed Ligation for Quantitative Proteomics. A徠ngewandte Chemie International Edition, 2013, 52(35): 9205-9209.

4)Houjiang Zhou#; Mingliang Ye#; Jing Dong; Eleonora Corradini; Alba Cristobal; Albert J R Heck*; Hanfa Zou*; Shabaz Mohammed*.Robust phosphoproteome enrichment using monodisperse microsphere–based immobilized titanium (IV) ion affinity chromatography. Nature Protocols, 2013, 8,3,461-480

5）ZhenzhenDeng; Jiawei Mao; Yan Wang; HanfaZou; Mingliang Ye*.Enzyme kinetics for complex system enables accurate determination of specificity constants of numerous substrates in a mixture by proteomics platform, Molecular & Cellular Proteomics, 2016,Papersin Press

6）Zhang, Zhang; Sun, Zhen; Zhu, Jun; Liu, Jing; Huang, Guang;Ye, Mingliang*;Zou, Hanfa*. High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis.Analytical Chemistry, 2014, 86(19): 9830-9837.

7）Liu, Fangjie;Ye, Mingliang*;Pan, Yanbo; Zhang, Yi; Bian, Yangyang; Sun, Zhen; Zhu, Jun; Cheng, Kai; Zou, Hanfa*. Integration of Cell Lysis, Protein Extraction, and Digestion into One Step for Ultrafast Sample Preparation for Phosphoproteome Analysis.Analytical Chemistry,2014, 86(14): 6786-6791.

8）Pan, Yanbo;Ye, Mingliang*;Zheng, Hao; Cheng, Kai; Sun, Zhen; Liu, Fangjie; Liu, Jing; Wang, Keyun; Qin, Hongqiang; Zou, Hanfa*. Trypsin-Catalyzed N-Terminal Labeling of Peptides with Stable Isotope-Coded Affinity Tags for Proteome Analysis.Analytical Chemistry, 2014, 86(2): 1170-1177.

9）Junfeng Huang, Hongqiang Qin, Zhen Sun, Guang Huang, Jiawei Mao, Kai Cheng, Zhang Zhang, Hao Wan, Yating Yao, Jing Dong, Jun Zhu, Fangjun Wang,Mingliang Ye*, Hanfa Zou. A peptide N-terminal protection strategy for comprehensive glycoproteome analysis using hydrazide chemistry based method.Scientific Reports, 2015, 5:10164, 1-15

10）Wang, Chunli; Ye, Mingliang*;Bian, Yangyang; Liu, Fangjie; Cheng, Kai; Dong, Mingming; Dong, Jing; Zou, Hanfa. Determination of CK2 Specificity and Substrates by Proteome-Derived Peptide Libraries. Journal of Proteome Research, 2013, 12(8): 3813-3821.

11）Deng, Zhenzhen， Ye, Mingliang*，Bian, Yangyang，Liu, Zheyi，Liu, Fangjie，Wang, Chunli; Qin, Hongqiang，Zou, Hanfa*，Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS, Chemical Communications, 2014, 50(90): 13960-13962.

12）FangjieLiu,HaoWan,ZhongshanLiu,HongweiWang,JiaweiMao, MingliangYe*,HanfaZou*. Preparation of Polypropylene Spin Tips Filled with ImmobilizedTitanium(IV) Ion Monolithic Adsorbent for RobustPhosphoproteomeAnalysis. Analytical Chemistry, 2016, 88, 5058-5064