王曉雲(山東農業大學教授)

1985年以來任教，先後獲得山東農業大學碩士和博士學位。

2002-2003在清華大學做訪問學者。

山東大學教授王曉雲

2008-2010年兩度到美國哈佛大學做高級訪問學者。

“Global Journal of Biochemistry”國際期刊編委。

“Comparative Biochemistry and Physiology”審稿人。

“International Journal of Biological Macromolecules”審稿人。

“Journal of Molecular Catalysis”審稿人。

研究方向

主要研究方向為蛋白質（酶）的結構與功能的關係，在克隆、表達基因的基礎上，利用定點突變技術，研究關鍵氨基酸殘基對蛋白質結構及功能的影響等，特別是逆境脅迫下蛋白質表達與氧化脅迫的關係，以及二硫鍵形成途徑中關鍵酶的研究等，也包括酶的抑制動力學及生物體內代謝調控酶的分離純化、性質研究及調控機理的研究。

一直以來從事生物化學與分子生物學、植物生理生化方面的科研與教學工作，著重研究酶的催化機制以及結構與功能的關係。主持承擔國家自然基金、高等學校博士學科點專項科研基金、山東省優秀中青年科學家科研獎勵基金、作物生物學國家重點實驗室基金等課題。

主要貢獻

作為第一作者或通訊作者在“Antioxidant and Redox Signalling”、“International Journal of Biochemistry and Cell Biology”、“International Journal of Biological Macromolecules”、“中國農業科學”等國內外學術刊物發表學術論文60多篇。

科研項目

1.農業部公益性行業科研專項“我國重要野生果樹資源的收集、評價與優異種質創新利用技術研究與示範”(201303093).。

2.國家自然科學基金《利用定點突變技術研究二硫鍵對植酸酶結構、催化活性及熱穩定性的作用》（No.30471261）。

3.國家自然科學基金《通過引入二硫鍵、增加糖基化位點等方式提高植酸熱穩定性的研究》（No.30940017）。

4.高等學校博士點專項基金：《糖基化及二硫鍵對植酸酶穩定性的研究》（No.20050434008）。

5.山東省中青年科學家科研獎勵基金《葉片衰老關鍵酶分離純化及調控衰老物質的研製與開發》。

科研成果

對酶的熱穩定性及二硫鍵在去摺疊和再摺疊過程中的作用，有較深入的研究，指導博士研究生6人，碩士研究生33人，作為第一作者或通訊作者，在“Antioxidant and Redox Signalling”、“International Journal of Biochemistry and Cell Biology” 、“International Journal of Biological Macromolecules”、“Journal of Integrative Plant Biology”、“Plant Science”、“中國農業科學”、“作物學報”等國內外學術刊物發表學術論文60多篇，其中SCI收錄30餘篇。

發表論文

Yu Z. B., Yang X. J., Du J. J., Wan C. M., Xu J. N., Wang W. J., Feng Y. G., and Wang X. Y.*, A homologue of vitamin K epoxide reductase in Solanum lycopersicum is involved in resistance to osmotic stress. Physiol Plant. 2015. DOI:10.1111/ppl.12376.

Yang X. J., Cui H. R., Yu Z. B., Du J. J., Xu J. N., and Wang X. Y.*, Key Amino Acids of Arabidopsis VKOR in the Activity of Phylloquinone Reduction and Disulfide Bond Formation. Protein Pept Lett. 2015, 22:81-86.

Xu J. N., Xing S. S., Cui H. R., Chen X. S., and Wang X. Y.*, Genome-wide identification and characterization of the apple (Malus domestica) HECT ubiquitin-protein ligase family and expression analysis of their responsiveness to abiotic stresses. Mol Genet Genomics. 2015. DOI:10.1007/s00438-015-1129-0.

Lu Y., Peng J. J., Yu Z. B., Du J. J., Xu J. N., and Wang X. Y.*, Thylakoid membrane oxidoreductase LTO1/AtVKOR is involved in ABA-mediated response to osmotic stress in Arabidopsis. Physiol Plant. 2015, 154:28-38.

Lu Y., Du J. J., Yu Z. B., Peng J. J., Xu J. N., and Wang X. Y.*, Identification of potential targets for thylakoid oxidoreductase AtVKOR/LTO1 in chloroplasts. Protein Pept Lett. 2015, 22:219-225.

Li F., Li W., Liu Y., Tong Y. X., Zhou P., Wang L., Chen C., Zeng L. Y., Wu Q. Y., Wang X. Y. *, and Xu K. L., Effects of the I682F mutation on JAK2's activity, structure and stability. Int J Biol Macromol. 2015, 79:118-125.

Du J. J., Zhan C. Y., Lu Y., Cui H. R., and Wang X. Y.*, The conservative cysteines in transmembrane domain of AtVKOR/LTO1 are critical for photosynthetic growth and photosystem II activity in Arabidopsis. Frontiers in Plant Science. 2015, 6:238.

Cui H. R., Zhang Z. R., Lv W., Xu J. N., and Wang X. Y.*, Genome-wide characterization and analysis of F-box protein-encoding genes in the Malus domestica genome. Molecular Genetics and Genomics. 2015, 290:1-12.

Jing X. L., Xu J., Fan M. N., Ma L., Huang X., Wang X. Y., Sun S. H., Zhu C. X., and Liu H. M., Mutation analysis of the RNA silencing suppressor NS1 encoded by avian influenza virus H9N2. Journal of General Virology. 2015, 96:1613-1618.

Zhan C. Y., Xu J. N., Li D., Liu B. B., Feng Y. G., and Wang X. Y. *, Overexpression of AtCBF1 in Sinningia speciosa Enhances Chilling Stress Tolerance. Research & Reviews: Journal of Botanical Sciences. 2015, S1：26-33.

Wu Q. Y., Guo H. Y., Geng H. L., Ru B. M., Cao J., Chen C., Zeng L. Y., Wang X. Y., Li F., and Xu K. L., T273 plays an important role in the activity and structural stability of arginine kinase. Int J Biol Macromol. 2014, 63:21-28.

Wang P., Li X. Z., Cui H. R., Feng Y. G., and Wang X. Y*., Identification and functional analysis of a novel parvulin-type peptidyl-prolyl isomerase from Gossypium hirsutum. Plant Physiol Biochem. 2014, 76C:58-66.

Wan C. M., Yang X. J., Du J. J., Lu Y., Yu Z. B., Feng Y. G., and Wang X. Y*., Identification and characterization of SlVKOR, a disulfide bond formation protein from Solanum lycopersicum, and bioinformatic analysis of plant VKORs. Biochemistry (Mosc). 2014, 79:440-449.

Geng H. L., Bian M. R., Liu Y., Cao J., Chen C., Wang Z. Y., Li Z. Y., Zeng L. Y., Wang X. Y. *, Wu Q. Y., and Xu K. L., The D14 and R138 ion pair is involved in dimeric arginine kinase activity, structural stability and folding. Int J Biol Macromol. 2014, 66:302-310.

Du F., Xu J. N., Zhan C. Y., Yu Z. B., and Wang X. Y.*, An obesity-like gene MdTLP7 from apple (Malus× domestica) enhances abiotic stress tolerance. Biochemical and biophysical research communications. 2014, 445:394-397.

Du F., Xu J. N., Li D., and Wang X. Y.*, The identification of novel and differentially expressed apple-tree genes under low-temperature stress using high-throughput Illumina sequencing. Mol Biol Rep. 2014, 42:569-580.

Yu Z. B., Lu Y., Du J. J., Peng J. J., and Wang X. Y.*, The chloroplast protein LTO1/AtVKOR is involved in the xanthophyll cycle and the acceleration of D1 protein degradation. J Photochem Photobiol B. 2013, 130C:68-75.

Lu Y., Wang H. R., Li H., Cui H. R., Feng Y. G., and Wang X. Y*., A chloroplast membrane protein LTO1/AtVKOR involving in redox regulation and ROS homeostasis. Plant Cell Rep. 2013, 32:1427-1440.

Li F., Wu Q. Y., and Wang X. Y., The amino acid residue L113 is involved in arginine kinase activity and structural stability. Int J Biol Macromol. 2013, 52:198-205.

Wu Q. Y., Li F., Wang X. Y. *, and Xu K. L., Evidence that the amino acid residue Ile121 is involved in arginine kinase activity and structural stability. Int J Biol Macromol. 2012, 51:369-377.

Wu Q. Y., Li F., Guo H. Y., Cao J., Chen C., Chen W., Zhao K., Zeng L. Y., Han Z. X., Li Z. Y., Wang X. Y. *, and Xu K. L., Amino acid residue E543 in JAK2 C618R is a potential therapeutic target for myeloproliferative disorders caused by JAK2 C618R mutation. Arch Biochem Biophys. 2012, 528:57-66.

Wu Q. Y., Li F., Wang X. Y. *, and Chen Z. J., Impact of inter-subunit interactions on the dimeric arginine kinase activity and structural stability. Arch Biochem Biophys. 2011, 512:61-68.

Wu Q. Y., Jin K. Z., Li F., Hu Z. Q., and Wang X. Y.*, Impact of intra-subunit interactions on the dimeric arginine kinase activity and structural stability. Int J Biol Macromol. 2011, 49:822-831.

Wang X. Y., Dutton R. J., Beckwith J., and Boyd D., Membrane topology and mutational analysis of Mycobacterium tuberculosis VKOR, a protein involved in disulfide bond formation and a homologue of human vitamin K epoxide reductase. Antioxid Redox Signal. 2011, 14:1413-1420.

Wang H. R., Zhu W. J., and Wang X. Y.*, Mechanism of inhibition of arginine kinase by flavonoids consistent with thermodynamics of docking simulation. Int J Biol Macromol. 2011, 49:985-991.

Jing X. L., Fan M. N., Jia G., Liu L. W., Ma L., Zheng C. C., Zhu X. P., Liu H. M., and Wang X. Y.*, A Multifunctional Protein Encoded by Turkey Herpesvirus Suppresses RNA Silencing in Nicotiana benthamiana. J Virol. 2011, 85:12792-12803.

Feng W. K., Wang L., Lu Y., and Wang X. Y.*, A protein oxidase catalysing disulfide bond formation is localized to the chloroplast thylakoids. FEBS J. 2011, 278:3419-3430.

Li F., Wu Q. Y., Sun Y. L., Ma N. N., Wang X. Y., and Meng Q. W., Evidence that the amino acid residue Cys117 of chloroplastic monodehydroascorbate reductase is involved in its activity and structural stability. Int J Biol Macromol. 2010, 46:350-355.

Wu X. Q., Zhu W. J., Lu Z. R., Xia Y., Yang J. M., Zou F., and Wang X. Y.*, The effect of rutin on arginine kinase: inhibition kinetics and thermodynamics merging with docking simulation. Int J Biol Macromol. 2009, 44:149-155.

Wu X. Q., Xu H., Yue H., Liu K. Q., and Wang X. Y.*, Inhibition kinetics and the aggregation of alpha-glucosidase by different denaturants. Protein J. 2009, 28:448-456.

Wu Q. Y., Li F., and Wang X. Y.*, Val65 plays an important role in the substrate synergism, structural stability and activity of arginine kinase. Int J Biol Macromol. 2009, 45:393-398.

Shi X. W., Sun M. L., Zhou B., and Wang X. Y.*, Identification, characterization, and overexpression of a phytase with potential industrial interest. Can J Microbiol. 2009, 55:599-604.

Zhou J. P., Yang Z. B., Yang W. R., Wang X. Y., Jiang S. Z., and Zhang G. G., Effects of a New Recombinant Phytase on the Performance and Mineral Utilization of Broilers Fed Phosphorus-Deficient Diets. The Journal of Applied Poultry Research. 2008, 17:331.

Wu Q. Y., Li F., and Wang X. Y.*, Evidence that amino-acid residues are responsible for substrate synergism of locust arginine kinase. Insect Biochem Mol Biol. 2008, 38:59-65.

Wu Q. Y., Li F., and Wang X. Y.*, Evidence that the amino acid residue P272 of arginine kinase is involved in its activity, structure and stability. Int J Biol Macromol. 2008, 43:367-372.

Zhu W. J., Li M., and Wang X. Y.*, Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active site. Int J Biol Macromol. 2007, 41:564-571.

Zhao D. M., Wang M., Mu X. J., Sun M. L., and Wang X. Y.*, Screening, cloning and overexpression of Aspergillus niger phytase (phyA) in Pichia pastoris with favourable characteristics. Lett Appl Microbiol. 2007, 45:522-528.

Xia Y., Park Y. D., Mu H., Zhou H. M., Wang X. Y.*, and Meng F. G., The protective effects of osmolytes on arginine kinase unfolding and aggregation. Int J Biol Macromol. 2007, 40:437-443.

Wu Q. Y., Li F., Zhu W. J., and Wang X. Y.*, Cloning, expression, purification, and characterization of arginine kinase from Locusta migratoria manilensis. Comp Biochem Physiol B Biochem Mol Biol. 2007, 148:355-362.

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