趙建國

中國科學院動物研究所大動物生殖與發育研究組組長

中科院動物所"百人計劃"引進海外傑出人才。2003年畢業於東北農業大學，獲得動物遺傳育種與繁殖專業博士學位；2003至2005年在上海交通大學醫學遺傳研究所任助理研究員，2005至2007年在美國新奧爾良大學任博士后研究人員；2007至2010年在美國密蘇里大學哥倫比亞分校任研究助理教授。2010年入選中國科學院現代農業創新基地“項目百人計劃”。主要研究方向為通過分子誘變或體細胞核移植轉基因技術策略來改善豬的農業生產性狀或構建用於生物醫學研究為目的的轉基因動物模型。現主持中國科學院“項目百人”計劃項目1項，2005年獲得上海“白玉蘭”科技基金1項。

目前的研究成果主要包括創建了攜帶有人P23H Rhodopsin突變基因的NIH近交系轉基因小型豬，視覺電生理檢測已經證實在不同的個體中均出現不同程度的視力下降，可以作為人的色素性視網膜炎的疾病模型；創建了攜帶有人抗凝血因子9, Furin蛋白和α-抗胰蛋白酶的轉基因豬，其中凝血因子9的表達水平在乳汁中達到了1克/升。首次報道利用組蛋白去乙醯化抑製劑-Scriptaid大幅提高了在生物醫學研究中具有廣泛應用價值的-NIH近交系小型豬的克隆效率。首次報道了利用鋅指核酶技術在大動物當中實現了GFP基因的高效成功敲除。

1.Whyte JJ*, Zhao JG*, Samuel M, Laughlin HM, Prather RS, Gene Targeting with Zinc Finger Nucleases to Produce Cloned eGFP Knockout Pigs. Mol Reprod Dev. 2011, Accepted.

2.Zhao JG, Whyte JJ, Prather RS. The effect of epigenetic regulation on somatic cell nuclear transfer. Cell. Cell Tissue Res. 2010, 341(1):13-21.

3.Zhao JG, Hao Y, Ross JW, Spate LD, Walters EM, Samuel MS, Rieke A, Murphy CN, Prather RS. Histone Deacetylase Inhibitors Improve In Vitro and In Vivo Developmental Competence of Somatic cell Nuclear Transfer Porcine Embryos. Cloning and stem cells, 2010;12(1):75-83

4.Ross JW, Prather RS, Whyte JJ, Zhao JG. Cloning and transgenics: progress and new approaches. Invited review by Centre for Agricultural Bioscience International 2009,4, No. 38

5.Zhao J, Ross JW, Hao Y, Spate LD, Walters EM, Samuel MS, Rieke A, Murphy CN, Prather RS. Significant Improvement in Cloning Efficiency of an Inbred Miniature Pig by Histone Deacetylase Inhibitor Treatment after Somatic Cell Nuclear Transfer. Biol Reprod. 2009 81: 525-530

6.Zhao M, Isom SC, Lin H, Hao Y, Zhang Y, Zhao J, Whyte JJ, Dobbs KB, Prather RS. Tracing the stemness of porcine skin-derived progenitors (pSKP) back to specific marker gene expression. Cloning Stem Cells. 2009 ;11(1):111-22

7.Miao YL, Sun QY, Zhang X, Zhao JG, Zhao MT, Spate L, Prather RS, Schatten H. Centrosome abnormalities during porcine oocyte aging. Environ Mol Mutagen. 2009; 50(8): 666-71

8.Yang XY, Zhao JG, Li H, Liu HF, Huang Y, Huang SZ, Zeng F, Zeng YT. Effect of individual heifer oocyte donors on cloned embryo development in vitro. Anim Reprod Sci. 2008 104(1):28-37

9.Yang XY, Li H, Ma QW, Yan JB, Zhao JG, Li HW, Shen HQ, Liu HF, Huang Y, Huang SZ, Zeng YT, Zeng F. Improved efficiency of bovine cloning by autologous somatic cell nuclear transfer. Reproduction. 2006 132(5):733-9

10.Zhao JG, Li H, Kong X, Tang ZQ. Association analysis of SNPs in the chicken UCP gene with growth and body composition traits. Canadian Journal of Animal Science. 2006, 86:345-350

11.Zhao JG, Li H, Wang YX, Meng H. The expression characterization of chicken uncoupling protein gene. Asian-Australasian Journal of Animal Science, 2005, 18 (11): 1552-1556

12.Yang XY, Zhao JG, Li HW, Li H, Liu HF, Huang SZ, Zeng YT. Improve the in vitro development of cloned bovine embryos by use of holstein-chinese yellow hybrid recipient oocytes recovered by ovum pick up. Theriogenology. 2005, 64(6):1263-72

13.Meng H, Li H, Zhao JG and Gu ZL. Differential expression of peroxisome proliferator-activated receptors alpha and gamma gene in various chicken tissues. Domestic Animal Endocrinology, 2005, 28:105-110