王連榮

2008-2011任美國麻省理工學院生物工程系博士后。

2011-至今任武漢大學藥學院教授。

科研方向

致力於研究一種核酸DNA骨架上的新型修飾——磷硫醯化修飾，這是首次發現的發生於磷酸骨架上的生理修飾，又稱為硫修飾。作為生命的物質基礎，DNA由碳、氫、氧、氮和磷五種元素組成的四種核苷酸構成，但是我們發現細菌能夠利用dnd基因簇對DNA磷酸骨架上非橋聯氧原子進行硫取代，形成序列特異性、空間構象專一性的DNA磷硫醯化硫修飾，而且這種生理修飾在自然界的細菌中廣泛存在，是細菌抵禦外來DNA入侵、保持自身遺傳穩定的新型免疫系統。DNA磷硫醯化硫修飾的發現被《環球科學》評為2007年度“全球十大科學新聞”。

承擔課題

（1）主持國家基金委優秀青年基金

（2）973課題負責人

（3）主持國家自然科學基金面上項目

（4）主持全國優秀博士論文專項基金

（5）主持教育部新世紀優秀人才專項基金

（6）主持湖北省自然科學基金

（7）參與國家自然科學基金重點項目

代表性論文

1.Cao B , Chen C , DeMott M , Cheng Q , Clark T, Xiong X, Zheng X, Butty V , Levine S, Yuan G, Boitano M, Luong K, Song Y, Deng Z, Zhou X, Turner S, Korlach K, You D*, Wang L*, Chen S*, Dedon P. Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences. Nature Communications. 2014, 5;5:3951. (co-corresponding author)

2.Gan R, Wu X, He W, Liu Z, Wu S, Chen C, Chen S, Xiang Q, Deng Z, Liang D, Chen S, Wang L. DNA phosphorothioate modifications influence the global transcriptional response and protect DNA from double-stranded breaks. Scientific Reports. 2014 Oct 16;4:6642.

3.Wang L, Chen S, Xu T, Taghizadeh K, Wishnok JS, Zhou X, Deng Z, Dedon PC. Phosphorothioation of DNA in bacteria by dnd genes. Nature Chemical Biology. 2007, 3(11):709-10.

4.Wang L, Chen S, Vergin KL, Giovannoni Sj. Chan SW, Demott MS, Taghizadeh K, Cordero OX, Cutler M, Timberlake S, Alm EJ, Polz MF, Pinhassi J, Deng Z, Dedon PC. DNA phosphorothioation is widespread and quantized in bacterial genomes. Proc Natl Acad Sci USA. 2011, 108(7):2963-8.

5.Wang L, Chen S, Xiao X, Huang X, You D, Zhou X, Deng Z. arsRBOCT arsenic resistance system encoded by linear plasmid pHZ227 in Streptomyces sp. Strain FR-008. Applied and Environmental Microbiology. 2006, 72(5):3738-42.

6.Wang L., Chen S., Deng Z. Phosphorothioation: an unusual post-replicative modification on the DNA backbone. Book Chapter. DNA replication. 2011.

7.You D, Wang L, Yao F, Zhou X, Deng Z. A novel DNA modification by sulfur:DndA is a NifS-like cysteine desulfurase capable of assembling DndC as an iron-sulfur cluster protein in Streptomyces lividans. Biochemistry. 2007, 46(20):6126-33.

8.Chen S, Wang L, Deng Z. Twenty years hunting for sulfur in DNA. Protein Cell. 2010, 191:14-21.

9.Chan W, Chen B, Wang L, Taghizadeh K, Demott MS, Dedon PC. Quantification of the 2-deoxyribonolactone and nucleoside 5’-aldehyde products of 2-deoxyribose oxidation in DNA and cells by isotope-dilution gas chromatography mass spectrometry: differential effects of gamma-radiation and Fe2+-EDTA. J Am Chem Soc.2010, 132(17):6145-53.

10.Xu T, Liang J, Chen S, Wang L, He X, You D, Wang Z, Li A, Xu Z, Zhou X, Deng Z. DNA phosphorothioation in Streptomyces lividans:mutational analysis of the dnd locus. BMC Microbiology, 2009, 9:41.

11.Chen S, Mao X, Shen Y, Zhou Y, Li J, Wang L, Tao X, Yang L, Wang Y, Zhou X, Deng Z, Wei D. Tailoring the P450 monooxygenase gene for FR-008/candicidin biosynthesis. Applied and Environmental Microbiology, 2009,75(6):1778-81.