共找到2條詞條名為楊明英的結果 展開

楊明英

浙江大學碩士生導師

楊明英,工學博士,浙江大學動物科學學院特種經濟動物科學系副研究員,碩士生導師。

人物經歷


1993年9月~1998年7月:浙江農業大學蠶學系農學學士。
1998年9月~2001年7月:浙江大學動物科學院 農學碩士。
2001年10月~2002年3月:東京農工大學工學部生命工學科進修生。
2002年4月~2005年3月:東京農工大學工學部生命工學科工學博士。
2005年4月~2007年8月:東京農工大學工學部生命工學科博士后研究員。
2007年9月~至今:浙江大學動物科學院生物資源應用研究所副研究員。

研究方向


1. 利用核磁共振手段(NMR)分析家蠶絲﹑野蠶絲以及蜘蛛絲蛋白的精細結構。
2. 在結構分析的基礎上,利用基因重組技術設計並表達功能性重組絲蛋白。
3. 利用成型加工技術,把絲蛋白以及重組絲蛋白加工成生物支架材料(三維支架,膜狀、海綿狀、納米纖維等)。
4. 通過細胞活性測試以及動物試驗,提供人體器官和組織的前體物。

主要貢獻


完成課題
1. 水溶性重組絲蛋白的設計、結構特徵及其對納米纖維形成關係的研究。
2. 水溶性重組絲蛋白的分子設計、表達及其結構特徵。
3.重組絲蛋白納米支架的研究(2010年浙江大學科研創新專項,主持)。
4.可控降解管型絲素蛋白尿道組織工程支架的研究(2008年國家自然科學基金,第二完成人)。
代表性論文
1. Yang, M., Y Shuai., W He., et al., Preparation of Porous Scaffolds from Silk Fibroin Extracted from the Silk Gland of Bombyx mori (B. mori). Int J Mol Sci, 2012. 13(6): p. 7762-75.
2. Shuai, YaJun., Pan Hui, Wen He, Si Jia Min, Liang Jun Zhu, Ming Ying Yang., Extraction of Silk Protein from Middle Silk Gland of B.mori for Preparation of 3-D Scaffold. Advanced Materials Research, 2012. 550-553: p. 1729-1736.
3. Min, S., X Gao., C Han., Y Chen., M Yang., et al., Preparation of a silk fibroin spongy wound dressing and its therapeutic efficiency in skin defects. Journal of Biomaterials Science, Polymer Edition, 2012. 23(1-4): p. 97-110.
4. Yang, M.Y., LJ Zhu., SJ Min., et al., Synthesis and Characterization of Novel Silk-Like Proteins Using Genetic Engineering Methods. Advanced Materials Research, 2011. 175: p. 258-265.
5. Zhang, H., L Deng., M Yang., et al., Enhancing Effect of Glycerol on the Tensile Properties of Bombyx mori Cocoon Sericin Films. Int J Mol Sci, 2011. 12(5): p. 3170-3181.
6. Zhang, Y., Y Ma., M Yang., et al., Expression, purification, and refolding of a recombinant human bone morphogenetic protein 2 in vitro. Protein Expr Purif, 2011. 75(2): p. 155-160.
7.M.Yang., J Kawamura, Z Zhu, K Yamauchi and T Asakura.Development of silk-like materials based on Bombyx mori and Nephila clavipes dragline silk fibroins.Polymer 2009, 50(1), 117-124.
8.M.Yang., C.Tanaka., K.Yamauchi., K.Ohgo., T.Asakura and M.Kurokawa.Silk-like materials constructed from sequences of Bombyx mori silk fibroin, fibronectin and elastin.Journal of Biomedical Materials research: Part A [J].2008, 84(2), 53-363.
9.M.Yang., T.Muto., D.Knight., A.M.Collins and T.Asakura.Synthesis and Characterization of Silklike Materials Containing the Calcium-Binding Sequence from Calbindin D9k or the Shell Nacreous Matrix Protein MSI60 [J].Biomacromolecules 2008,9(1), 416 – 420.
10.H.Zhang., M.Yang., S.Min., Q.Feng., X .Gao., L.Zhu.Preparation and characterization of a novel spongy hydrogel from aqueous Bombyx mori sericin[J].E-Polymers, 2008, 066.
11.M.Yang., K.Yamauchi., M.Kurokawa and T.Asakura.Design of silk-like biomaterials inspired by mussel adhesive protein [J].Tissue Engineering 2007,13(12), 2941-7.
12.T.Asakura., J.Yao., M.Yang and H., Hirose.Structure of the Spinning Apparatus and Molecular Dynamics Calculation on the Conformation of Silk Fibroin in the Silkworm Samia cynthia ricini [J].Polymer 2007,48 (7), 2064-2070.
13.T.Asakura., H.Sato., F.Moro., M.Yang., AM.Collins and D.Knight.Solid-state NMR Analysis of (GA)3S(AG)3D(GA)3S(AG)3D(GA)3S(AG)3, a peptide with a lamellar structure and a Ca binding site, and production of TS[(AG)3D(GA)3S]16 in E .coli.Macromolecules 2007,40 (25), 8983-8990.
14.M.Yang., Y.Nakazawa, David Knight and Tetsuo Asakura.Structure of the Model Peptides with the Sequence characteristic to Nephila clavipes dragline silk (MaSp1) Studied using Conformation-dependent 13C Chemical Shift in the Solid State [J].Biomacromolecules 2005,6, 3220-3226.
15.Yang and T.Asakura.Design, expression and solid-state NMR characterization of silk-like proteins constructed from sequences from spider silk, Samia cynthia ricini and Bombyx mori silk fibroins [J].Journal of Biochemistry 2005,137, 721-729.
16.T.Asakura, M.Yang., T.Kawase and Y.Nakazawa.13C Solid state NMR study of structural heterogeneity in peptides containing both polyalanine and repeated GGA sequences as a local structural model of Nephila clavipes dragline silk (MaSp1) [J].Macromolecules 2005,38, 3356-3363 .
17.M.Yang, J.Yao., M.Sonoyama and T.Asakura.Spectroscopic characterization of heterogeneous structure of Samia cynthia ricini silk fibroin induced by stretching and molecular dynamics simulation [J].Macromolecules 2004,37, 3497-3504.
18.T.Asakura, M.Yang and T.Kawase.Structure of characteristic sequences in Nephila clavipes dragline silk (MaSp1) studied with 13C solid state NMR [J].Polymer J.2004,36,999-1003.
19.T.Asakura., C.Tanaka., M.Yang., J.Yao and M.Kurokawa.Production and characterization of a silk-like hybrid protein, based on the polyalanine region of Samia cynthia ricini silk fibroin and a cell adhesive region derived from fibronectin [J].Biomaterials 2004,25, 617-624.
20.T.Asakura., K.Nitta., M.Yang., J.Yao., Y.Nakazawa and David L.Kaplan.Synthesis and characterization of chimeric silkworm.Biomacromolecules 2003,4, 815-820.