楊明英

1993年9月～1998年7月：浙江農業大學蠶學系農學學士。

1998年9月～2001年7月：浙江大學動物科學院 農學碩士。

2001年10月～2002年3月：東京農工大學工學部生命工學科進修生。

2002年4月～2005年3月：東京農工大學工學部生命工學科工學博士。

2005年4月～2007年8月：東京農工大學工學部生命工學科博士后研究員。

2007年9月～至今：浙江大學動物科學院生物資源應用研究所副研究員。

1. 利用核磁共振手段(NMR)分析家蠶絲﹑野蠶絲以及蜘蛛絲蛋白的精細結構。

2. 在結構分析的基礎上，利用基因重組技術設計並表達功能性重組絲蛋白。

3. 利用成型加工技術，把絲蛋白以及重組絲蛋白加工成生物支架材料（三維支架，膜狀、海綿狀、納米纖維等）。

4. 通過細胞活性測試以及動物試驗，提供人體器官和組織的前體物。

完成課題

1. 水溶性重組絲蛋白的設計、結構特徵及其對納米纖維形成關係的研究。

2. 水溶性重組絲蛋白的分子設計、表達及其結構特徵。

3.重組絲蛋白納米支架的研究（2010年浙江大學科研創新專項，主持）。

4.可控降解管型絲素蛋白尿道組織工程支架的研究（2008年國家自然科學基金，第二完成人）。

代表性論文

1. Yang, M., Y Shuai., W He., et al., Preparation of Porous Scaffolds from Silk Fibroin Extracted from the Silk Gland of Bombyx mori (B. mori). Int J Mol Sci, 2012. 13(6): p. 7762-75.

2. Shuai, YaJun., Pan Hui, Wen He, Si Jia Min, Liang Jun Zhu, Ming Ying Yang., Extraction of Silk Protein from Middle Silk Gland of B.mori for Preparation of 3-D Scaffold. Advanced Materials Research, 2012. 550-553: p. 1729-1736.

3. Min, S., X Gao., C Han., Y Chen., M Yang., et al., Preparation of a silk fibroin spongy wound dressing and its therapeutic efficiency in skin defects. Journal of Biomaterials Science, Polymer Edition, 2012. 23(1-4): p. 97-110.

4. Yang, M.Y., LJ Zhu., SJ Min., et al., Synthesis and Characterization of Novel Silk-Like Proteins Using Genetic Engineering Methods. Advanced Materials Research, 2011. 175: p. 258-265.

5. Zhang, H., L Deng., M Yang., et al., Enhancing Effect of Glycerol on the Tensile Properties of Bombyx mori Cocoon Sericin Films. Int J Mol Sci, 2011. 12(5): p. 3170-3181.

6. Zhang, Y., Y Ma., M Yang., et al., Expression, purification, and refolding of a recombinant human bone morphogenetic protein 2 in vitro. Protein Expr Purif, 2011. 75(2): p. 155-160.

7．M．Yang., J Kawamura, Z Zhu, K Yamauchi and T Asakura．Development of silk-like materials based on Bombyx mori and Nephila clavipes dragline silk fibroins．Polymer 2009, 50(1), 117-124.

8．M．Yang., C．Tanaka., K．Yamauchi., K.Ohgo., T．Asakura and M．Kurokawa．Silk-like materials constructed from sequences of Bombyx mori silk fibroin, fibronectin and elastin．Journal of Biomedical Materials research: Part A [J]．2008, 84(2), 53-363.

9．M．Yang., T．Muto., D．Knight., A.M．Collins and T．Asakura．Synthesis and Characterization of Silklike Materials Containing the Calcium-Binding Sequence from Calbindin D9k or the Shell Nacreous Matrix Protein MSI60 [J]．Biomacromolecules 2008，9(1), 416 – 420.

10．H．Zhang., M．Yang., S.Min., Q．Feng., X .Gao., L.Zhu．Preparation and characterization of a novel spongy hydrogel from aqueous Bombyx mori sericin[J]．E-Polymers, 2008, 066.

11.M．Yang., K．Yamauchi., M．Kurokawa and T．Asakura．Design of silk-like biomaterials inspired by mussel adhesive protein [J]．Tissue Engineering 2007，13(12), 2941-7.

12．T．Asakura.,　J．Yao., M．Yang and H., Hirose．Structure of the Spinning Apparatus and Molecular Dynamics Calculation on the Conformation of Silk Fibroin in the Silkworm Samia cynthia ricini [J]．Polymer 2007，48 (7), 2064-2070．

13.T．Asakura., H．Sato., F．Moro., M．Yang., AM．Collins and D．Knight．Solid-state NMR Analysis of (GA)3S(AG)3D(GA)3S(AG)3D(GA)3S(AG)3, a peptide with a lamellar structure and a Ca binding site, and production of TS[(AG)3D(GA)3S]16 in E .coli．Macromolecules 2007，40 (25), 8983-8990.

14.M.Yang., Y．Nakazawa, David Knight and Tetsuo Asakura．Structure of the Model Peptides with the Sequence characteristic to Nephila clavipes dragline silk (MaSp1) Studied using Conformation-dependent 13C Chemical Shift in the Solid State [J]．Biomacromolecules 2005，6, 3220-3226.

15.Yang and T．Asakura．Design, expression and solid-state NMR characterization of silk-like proteins constructed from sequences from spider silk, Samia cynthia ricini and Bombyx mori silk fibroins [J]．Journal of Biochemistry 2005，137, 721-729．

16.T．Asakura, M．Yang., T．Kawase and Y．Nakazawa．13C Solid state NMR study of structural heterogeneity in peptides containing both polyalanine and repeated GGA sequences as a local structural model of Nephila clavipes dragline silk (MaSp1) [J]．Macromolecules 2005，38, 3356-3363 .

17．M．Yang, J．Yao., M．Sonoyama and T．Asakura．Spectroscopic characterization of heterogeneous structure of Samia cynthia ricini silk fibroin induced by stretching and molecular dynamics simulation [J]．Macromolecules 2004，37, 3497-3504.

18．T．Asakura, M．Yang and T．Kawase．Structure of characteristic sequences in Nephila clavipes dragline silk (MaSp1) studied with 13C solid state NMR [J]．Polymer J．2004，36,999-1003．

19．T．Asakura., C．Tanaka., M．Yang., J．Yao and M．Kurokawa．Production and characterization of a silk-like hybrid protein, based on the polyalanine region of Samia cynthia ricini silk fibroin and a cell adhesive region derived from fibronectin [J]．Biomaterials 2004，25, 617-624.

20．T．Asakura., K．Nitta., M．Yang., J．Yao., Y．Nakazawa and David L．Kaplan．Synthesis and characterization of chimeric silkworm.Biomacromolecules 2003，4, 815-820.