李爽

2006.8-2007.6 廣葯集團廣州拜迪生物醫藥有限公司，擔任科研開發部部長。

2007-8-2010.8華南理工大學生物科學與工程學院講師。

現任華南理工大學生物科學與工程學院教授。

科研項目

關於改善甘油脫水酶機理性失活的研究，國家自然科學基金青年科學基金項目。

科研成果

對酶的設計、表達和改性有深入認識，在定向進化技術的應用具有豐富的經驗。所設計合成的可紫外/熱誘導的E. coli自動裂解體系能夠實現大腸桿菌胞內酶的表達和高通量篩選，且成本低廉、操作簡單。且已成功應用於酶的定向進化工作，現已授權給瑞士的 DualSystems Biotech公司進行世界範圍銷售(貨號：P08001)。該項工作還獲得了第七屆亞太生物化學工程會議的“Outstanding Poster Presentation”獎勵。此外利用DNA重組技術，建立了一套有效的蛋白結構功能單元的快速篩選/選擇方法。該方法構思巧妙，不具有基因（或蛋白）特異性，不需要了解蛋白結構—功能關係，操作簡單，是尋找蛋白可摺疊單元的通用方法。該項工作已經受邀發表在Methods in Molecular Biology（Humana Press, a part of Springer Science）一書中。

（1）利用合成生物學的工具，指導改造或合成新型的或改良的生物學體系（細胞工廠），以生產生物能源燃料及生物醫藥化學品。

（2）通過代謝途徑(網路)不斷的改造和優化，系統提高目標產物產量，或降低毒害中間產物的產生與積累。

（3）利用蛋白/酶改造技術，有針對性的改造細胞工廠內關鍵蛋白/酶，提升反應效率。

專利

林章凜，李爽，蔡真, 病毒表面蛋白基因分解和表面蛋白的大規模製備，中國專利。

林章凜，李爽，陳勇，一種基於篩選方法獲得具有獨立結構/功能的蛋白片段的方法。

林章凜，李爽，徐麗華，依賴紫外誘導裂解基因的胞內酶高通量篩選體系。

林章凜，陳勇，李爽，一種基於選擇方法獲得具有獨立結構/功能的蛋白片段的方法。中國專利

林章凜，徐麗華，李爽，任川，依賴熱誘導裂解基因的胞內酶高通量篩選體系。

論文

1. Zhu M, Zhang L, Yang F, Cha Y, Li S*, Zhuo M, Huang S, Li J. A recombinant β-mannanase from Thermoanaerobacterium aotearoense SCUT27: Biochemical characterization and its thermostability improvement. J Agr Food Chem 2020, 68: 818-825.

2. Chen H, Zhu C, Zhu M, Xiong J, Ma H, Zhuo M, and Li S*. High production of valencene in Saccharomyces cerevisiae through metabolic engineering. Microb Cell Fact. 2019, 18:195.

3. Yuan P, Dong J, Zhao W, Zhuo M, Li S*, Huang S, and Li J. Qualitative and quantitative assessment of genotoxins using SRRz lysis reporter under the control of a newly designed SOS responsive promoter in Escherichia coli. RSC Advances. 2019, 9:35662-35670.

4. Huang S, Huang X, Li S, Zhu M, Zhuo M. MHC class I allele diversity in cynomolgus macaques of Vietnamese origin. PeerJ. 2019, 7:e7941.

5. Ye F, Liu S, Yang Y, Zhao T, Li S, Zhou T, et al. Identification of the major metabolites of (R)-salbutamol in human urine, plasma and feces using ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. J Sep Sci. 2019, 42:3200-8.

6. Ouyang, X,Cha Y, Li W, Zhu C, Zhu M, Li S*, Zhuo M, Huang S and Li J. Stepwise engineering of Saccharomyces cerevisiae to produce (+)-valencene and its related sesquiterpenes. RSC Advances, 2019, 9:30171-30181.

7. Xu T, Huang X, Li Z, Ki Lin C S, Li S*, Enhanced purification efficiency and thermal tolerance of Thermoanaerobacterium aotearoense β-Xylosidase through aggregation triggered by short peptides. J Agr Food Chem 2018, 66: 4182-4188.

8. Xu H, Tong N, Huang S, Hayat W, Fazal S, Li J, Li S, Yan J, Zhang Y, Simultaneous autotrophic removal of sulphate and nitrate at different voltages in a bioelectrochemical reactor (BER): Evaluation of degradation efficiency and characterization of microbial communities. Bioresour Technol 2018, 265:340-348.

9. Xu H, Tong N, Huang S, Zhou S, Li S, Li J, Zhang Y, Degradation of 2,4,6-trichlorophenol and determination of bacterial community structure by micro-electrical stimulation with or without external organic carbon source. Bioresour Technol 2018, 263:266-272.

10. Du C, Abdullah JJ, Greetham D, Fu D, Yu M, Ren L, Li S, Lu D. Valorization of food waste into biofertiliser and its field application. J Clean Prod 2018, 187:273-284.

11. Zhu M, Fan W, Cha Y, Yang X, Lai Z, Li S*, Wang, X. Dynamic cell responses in Thermoanaerobacterium sp. under hyperosmotic stress. Sci Rep, 2017, 7:10088.

12. Yang X, Zhu M, Huang X, Lin CSK, Wang J, Li S*, Valorisation of mixed bakery waste in non-sterilized fermentation for l-lactic acid production by an evolved Thermoanaerobacterium sp. strain. Bioresource Technol, 2015, 198:47-54.

13. Zhu M, Lu Y, Wang J, Li S*, Wang X, Carbon catabolite repression and the related genes of ccpA, ptsH and hprK in Thermoanaerobacterium aotearoense. PLoS ONE, 2015, 10:e0142121. (JCR Q1, IF2.776)

14. Huang X, Li Z, Du C, Wang J, Li S*, Improved expression and characterization of a multi-domain xylanase from Thermoanaerobacterium aotearoense SCUT27 in Bacillus subtilis. J Arg Food Chem, 2015, 63:6430-6439.

15. Yang F, Yang X, Li Z, Du C, Wang J, Li S*, Overexpression and characterization of a glucose-tolerant β-glucosidase from T. aotearoense with high specific activity for cellobiose. Appl Microbiol Biotechnol, 2015, 99:8903-8915.

16. Yang X, Huang A, Peng J, Wang J, Wang X, Lin Z*, Li S*, Self-assembly amphipathic peptides induce active enzyme aggregation that dramatically increases the operational stability of nitrilase, RSC Advances, 2014, 4: 60675-60684.

17. Lai Z, Zhu M, Yang X, Wang J, Li S* Optimization of key factors affecting hydrogen production from sugarcane bagasse by a thermophilic anaerobic pure culture. Biotechnol Biofuels 2014, 7:119. (JCR Q1, IF3.6.22)

18. Ai H, Zhang J, Yang M, Yu P, Li S, Zhu M, Dong H, Wang S, Wang J: Draft Genome Sequence of an Anaerobic, Thermophilic Bacterium, Thermoanaerobacterium aotearoense SCUT27, Isolated from a Hot Spring in China. Genome Announc 2014, 2.

19. Yang X, Lai Z, Lai C, Zhu M, Li S*, Wang J*, Wang X: Efficient production of l-lactic acid by an engineered Thermoanaerobacterium aotearoense with broad substrate specificity. Biotechnol Biofuels 2013, 6:124.

20. Xu W, Chooi YH, Choi JW, Li S, Vederas JC, Da Silva NA, Tang Y: LovG: the thioesterase required for dihydromonacolin L release and lovastatin nonaketide synthase turnover in lovastatin biosynthesis. Angew Chem Int Ed Engl 2013, 52:6472-6475.

21. Li L, Ai H, Zhang S, Li S, Liang Z, Wu ZQ, Yang ST, Wang JF: Enhanced butanol production by coculture of Clostridium beijerinckii and Clostridium tyrobutyricum. Bioresour Technology 2013, 143:397-404.

22. Liang ZX, Li L, Li S, Cai YH, Yang ST, Wang JF: Enhanced propionic acid production from Jerusalem artichoke hydrolysate by immobilized Propionibacterium acidipropionici in a fibrous-bed bioreactor. Bioprocess Biosyst Eng 2012, 35:915-921. IF=1.82

23. Li S, Yang X, Yang S, Zhu M, Wang X: Technology prospecting on enzymes: Application, marketing and engineering. Comput Struct Biotechnol J 2012, 2:e201209017.

24. Cai YH, Liang ZX, Li S, Zhu MJ, Wu ZQ, Yang ST, Wang JF: Bioethanol from fermentation of cassava pulp in a fibrous-bed bioreactor using immobilized Δldh, a genetically engineered Thermoanaerobacterium aotearoense. Biotechnol Bioproc Eng 2012, 17:1270-1277.

25. Cai Y, Lai C, Li S, Liang Z, Zhu M, Liang S, Wang J: Disruption of lactate dehydrogenase through homologous recombination to improve bioethanol production in Thermoanaerobacterium aotearoense. Enzyme Microb Tech 2011, 48:155-161.

26.Li S, Lai C, Cai Y, Yang X, Yang S, Zhu M, Wang J, Wang X: High efficiency hydrogen production from glucose/xylose by the ldh-deleted Thermoanaerobacterium strain. Bioresour Technol 2010, 101:8718-8724.

本科課程：《遺傳學》《基因診斷與基因治療》《細胞生物學實驗》《生物化學實驗》。

研究生課程：《科學實驗設計與實驗技能培訓》。

2012.9-2013.9美國加州大學洛杉磯分校(UCLA)化學與分子工程系訪問學者。