李文化

職稱職務：副教授，博士生導師

學科專業：細胞生物學

研究方向：腫瘤信號轉導及藥物治療

主要學習及工作經歷

1992.9－1996.6：中南民族大學，學士；

1996.7－1999.8：湖北省農業科學院，實習研究員

1999.9－2004.7：武漢大學生命科學學院，博士；

2004.11－2008.2：哈佛大學醫學院，博士后；

2徠008.3－至今：武漢大學生命科學學院，副教授。

中國細胞生物學學會理事

湖北省細胞生物學會理事、秘書長

武漢市藥理學會理事

1.原癌基因和抑癌基因在腫瘤發生髮展中的信號轉導及調控關係；

2.針對特定靶位點的抗癌藥物分子機理研究。

承擔課程（本科生、研究生）

《微生物學實驗》，生科院、醫學院本科生；

《人類重大疾病的研究前沿》，生科院研究生。

主要研究領域

1. 原癌基因和抑癌基因在腫瘤發生髮展中的信號轉導及調控關係；

2. 針對特定靶位點的抗癌藥物分子機理研究。

研究項目：

1. 主持：國家自然科學基金面上項目 2013-2016年；

2. 主持：國家自然科學基金面上項目 2011-2013年；

3. 主持：國家自然科學基金面上項目 2009-2011年；

4. 主持：留學回國人員科研啟動基金，2010-2012年；

5. 主持：國家新葯創製重大專項(2011ZX09102-001-32), 2011-2013年；

6. 主持：國家新葯創製重大專項 子課題(2010ZX09401), 2011-2013；

7. 主持：湖北省自然科學基金(2008CDB080), 2009-2010年；

8. 主持：武漢市青年科技晨光計劃 2009-2011年；

9. 主持：中央高校基本科研專項資金 2010-2011年；

10. 參入：國家重點基礎研究發展計劃（973計劃） (2010CB529800), 2010-2015年；

11. 參入：中央高校基本科研專項資金（創新群體）， 2012-2013年。

（*通訊作者）

1. Jiali Wan, Jia-Hong Wang, Ting Liu, Zhixiong Xie, Xue-Feng Yu,Wenhua Li*. Surface chemistry but not aspect ratio mediates the biological toxicity of gold nanorods in vitro and in vivo.Scientific Reports.2015, 5:11398; doi: 10.1038/srep11398.

2. Ting Liu, Qiuxu Men, Guixian Wu, Chunrong Yu, Zan Huang, Xin Liu*,Wenhua Li*. Tetrandrine inhibits proliferation and induces autophagy mediate differentiation by activating ROS and Notch1 signaling in NB4 leukemia cells.Oncotarget.2015, 6:7992-8006.

3. Haiqing Wang, Ting Liu, Lu Li, Qin Wang, Chunrong Yu, Xin Liu*,Wenhua Li*. Tetrandrine is a potent cell autophagy agonist via activated intracellular reactive oxygen species.Cell & Bioscience. 2015 5:4.

4. Liufeng Mei, Yicheng Chen, Zhimeng Wang, Jian Wang, Jiali Wan,Chunrong Yu, Xin Liu,Wenhua Li*.Synergistic antitumor effects of tetrandrine and chloroquine combination therapy in human cancer.British Journal of Pharmacology.2015, 172:2232–45

5. Jingru Li, Yicheng Chen, Jiali Wan, Xin Liu, Chunrong Yu,Wenhua Li*. ABT-263 enhances sorafenib-inducedapoptosis associated with Akt activity and the expression of Bax and p21 in human cancer cells.British Journal of Pharmacology.2014, 171:3182-95.

6. Ke Gong, Zhenxing Zhang, Yicheng Chen,Hong-Bing Shu,Wenhua Li*. Extracellular signal-regulated kinase, receptor interacting protein, and reactive oxygen species regulate shikonin-induced autophagy in human hepatocellular carcinoma.European Journal of Pharmacolgy. 2014, 738:142-52.

7. Yu Song, Ke Gong, Hong Yan, Wei Hong, Le Wang, Yingliang Wu,Wenhua Li*, Wenxin Li*, Zhijian Cao*. Sj7170, a serine protease peptide inhibitor from the venom of the scorpionScorpiops jendeki, promotes tumorigenesis and metastasis of human glioblastoma cellsin vitroandin vivo.Journal of Biological Chemistry.2014, 289:11667-80.

8. Jiali Wan, Ting Liu, Liufeng Mei, Jingru Li, Ke Gong, Chunrong Yu,Wenhua Li*. Synergistic antitumor activity of sorafenib in combination with tetrandrine is mediated by reactive oxygen species (ROS)/Akt signaling.British Journal of Cancer. 2013,109:342-50.

9. Yan Zhang,Wenhua Li*, Liming Wang, Ping Shen, Zhixiong Xie. Adenosine monophosphate affects competence development and plasmid DNA transformation in Escherichia coli.Current Microbiology. 2013,67:550-6.

10. Hai-Ming Hu, Yicheng Chen, Lu Liu, Chuan-Geng Zhang, Wei Wang, Ke Gong, Zan Huang, Ming-Xiong Guo, Wen-Xin Li* andWenhua Li*. C1orf61 acts as a tumor activator in human hepatocellular carcinoma and is associated with tumorigenesis and metastasis.FASEB Journal. 2013, 27:163-73.

11. Ke Gong, Chao Chen, Yao Zhan, Yan Chen, Zebo Huang,Wenhua Li*. Autophagy-related gene 7 (Atg7) and reactive oxygen species (ROS)/ extracellular-signal-regulated kinase (ERK) regulate tetrandrine-induced autophagy in human hepatocellular carcinoma.Journal of Biological Chemistry.2012, 287:35576-88.

12. Yao Zhan, Ke Gong, Chao Chen, Haiqing Wang,Wenhua Li*. p38 MAP kinase functions as a switch in MS-275 induced reactive oxygen species-dependent autophagy and apoptosis in human colon cancer cells.Free Radical Biology and Medicine. 2012, 53:532-43.

13. Ke Gong, Jia Xie, Hong Yi,Wenhua Li*. CS055 (Chidamide/HBI-8000), a novel histone deacetylase inhibitor, induces G1-arrest, ROS-dependent apoptosis and differentiation in human leukemia cells.Biochemical Journal.2012, 443:735-46.

14. Guan Wang, Yao Zhan, Haiqing Wang,Wenhua Li*. ABT-263 sensitizes TRAIL-resistant hepatocarcinoma cells by down-regulating the Bcl-2 family of anti-apoptotic protein.Cancer Chemotherapy and Pharmacology. 2012, 69:799-805.

15. Ke Gong,Wenhua Li*. Shikonin, a Chinese plant derived naphthoquinone induces apoptosis in hepatocellular carcinoma cells through Reactive Oxygen Species. A potential new treatment for hepatocellular carcinoma.Free Radical Biology and Medicine. 2011, 51:2259-7.

16. Chaoyang Liu, Ke Gong, Xin Mao,Wenhua Li*. Tetrandrine induces apoptosis by activating reactive oxygen species and repressing Akt activity in human hepatocellular carcinoma.International Journal of Cancer. 2011, 129:1519-31.

17.Wenhua Li, Chin-Lee Wu, Phillip Febbo, Aria F. Olumi. Stromally expressed c-Jun regulates proliferation of prostate epithelial Cells.American Journal of Pathology. 2007, 171: 1189-1198

18.Wenhua Li, Xiaoping Zhang, Aria F. Olumi. MG-132 sensitizes TRAIL-resistant prostate cancer cells by activating c-Fos/c-Jun heterodimers and repressing c-FLIP(L).Cancer Research. 2007, 67:2247-2255.

19.Li Wenhua, Xie Haiyan, Xie Zhixiong, Lu Zhexue, Ou Jianhong, Chen Xiangdong, Shen Ping. Exploring developing mechanism of Escherichia coli competence using quantum dots as fluorescent probes.Journal of Biochemical and Biophysical Methods. 2004, 58:59-66.

20.Li Wenhua, Zhao Ruming, Xie Zhixiong, Shen Ping. Effects of La on growth, transformation and gene expression of Escherichia coli.Biological Trace Element Research. 2003, 94:167-177.

21.Li Wenhua,Liu Yi, Zhao Ruming, Xie Zhixiong, Shen Ping, Qu Songsheng. Effects of La on the competence of Escherichia coli as studied by microcalorimetry.Biological Trace Element Research. 2003, 91:267-75.