吳世華

1994.9-1998.7昆明理工大學環境工程與化學工程系精細化工專業，工學學士；

1998.9-2001.3 昆明理工大學生物與化學工程學院應用化學專業，工學碩士；

2001.3-2004.3浙江大學理學院化學系有機化學專業,，理學博士。

2004.4-2006.12浙江大學生命科學學院，講師；

2006.12-現在 浙江大學生命科學學院，副教授。

天然產物是新葯研究的重要來源。迄今為止，幾乎一半以上的臨床抗癌藥物和抗感染藥物都是來自天然產物。近年來，隨著基因組學、蛋白組學和代謝組學以及系統生物學的快速發展，天然產物的研究迎來了新的歷史性機遇，發現和發展新的天然產物及其分離製備方法，揭示其新的作用機制和應用價值成為當前研究的一大熱點。工作主要集中於：抗腫瘤天然產物（生物毒素）的提取分離、結構鑒定、作用機制及其應用研究；天然產物在癌症預防中的作用及機制；中藥配伍機制在天然抗腫瘤先導化合物優化中的應用（即組合的研究策略用於癌症）；特殊生物資源的高效利用和開發。我們試圖通過新的高效逆流色譜及相關色譜技術，分離和鑒定一些結構新穎的具有抗腫瘤活性的天然產物（生物毒素）；通過代謝組學和蛋白質組學等方法發現一些具有潛在藥物價值的天然產物；通過上游和下游的細胞生物學激活或抑制，發現一些天然產物的新的作用通路，為天然產物的抗腫瘤應用奠定基礎。

近年來，主持承擔國家自然科學基金、浙江省自然基金及企業橫向項目等多項課題的研究，在國內外發表相關論文二十餘篇，申請和授權各類專利10餘項。

1. Wu DF, Jiand XH, Wu SH*, Direct purification oftanshinonesfromSalvia miltiorrhizaBungeby high-speed counter-current chromatography without presaturation of the two-phase solvent mixture,Journal of Separation Science, 2009,accepted.

2. Yang L, Wu DF, Luo KW, Wu SH*, Wu P. Andrographolide enhances 5-fluorouracil-induced apoptosis via caspase-8-dependent mitochondrial pathway involving p53 participation in hepatocellular carcinoma (SMMC-7721) cells. Cancer Letters 2009;276(2):180-8.

3. F.Y. Liu, K.W. Luo, Z.M. Yu, N.N. Co, S.H. Wu,P. Wu, K.P. Fung, T.T. Kwok, Suillin from the mushroom Suillus placidus as potent apoptosis inducer in human hepatoma HepG2 cells. Chemico-Biological Interactions 181 (2009) 168-174.

4. Wu SH*,Yang L, Gao YA, Liu XY, Liu FY. Multi-channel counter-current chromatography for high-throughput fractionation of natural products for drug discovery. Journal of Chromatography A 2008;1180(1-2):99-107.

5. Wang TT, Jiang XH, Yang L, Wu SH*. pH-gradient counter-current chromatography isolation of natural antioxidant chlorogenic acid from Lonicera japonica Thumb. using an upright coil planet centrifuge with three multi-layer coils connected in series. Journal of Chromatography A 2008;1180(1-2):53-8.

6. Yang L, Wu SH*,Zhang QH, Liu F, Wu P. 23,24-Dihydrocucurbitacin B induces G(2)/M cell-cycle arrest and mitochondria-dependent apoptosis in human breast cancer cells (Bcap37). Cancer Letters 2007;256:267-78.

7. Ren G, Yu ZM, Chen YL, Wu SH, Fu CX.Sesquiterpene lactones from Saussurea alata. Natural Product Research 2007;21(3):221-6.

8. Qi Xiaopeng，Wu Zhongchang, Li Jinhui, Mo Xiaorong, Wu Shihua, Chu Jun, Wu Ping. AtCYT-INV1, a neutral invertase, is involved in osmotic stress-induced inhibition on lateral root growth in Arabidopsis. Plant Molecular Biology 2007;64(5):575-87.

9. Zhou H, Zheng ZG, Wu徠 SH, Tai YP, Cao XJ, Pan YJ. Separation and characterization of clindamycin and related impurities in bulk drug by high-performance liquid chromatography-electrospray tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 2006;41(4):1116-23.

10. Wu SH, Tai YP, Pan YJ, Sun CR. Effect of gravitational force on type-J counter-current chromatography by mathematical analysis. Journal of Chromatography A 2006;1103(2):243-7.

11. Liu FY, Wu SH, Chen YL, Yang L, Wu P. Verticillin chloroform solvate. Acta Crystallographica Section E-Structure Reports Online 2006;62:O974-O6.

12. Wu SH, Sun CR, Pan YJ. Preparative counter-current chromatography purification of valrubicin (AD-32) from crude synthetic drug using upright coil planet centrifuge with four multilayer coils connected in series. Journal of Chromatography A 2005;1076(1-2):207-11.

13. Wu SH, Sun CR, Wang KW, Pan YJ. Preparative isolation and purification of celastrol from Celastrus orbiculatus Thunb. by a new counter-current chromatography method with an upright coil planet centrifuge. Journal of Chromatography A 2004;1028(1):171-4.

14. Wu SH, Sun CR, Pei SF, Lu YB, Pan YJ. Preparative isolation and purification of amides from the fruits of Piper longum L. by upright counter-current chromatography and reversed-phase liquid chromatography. Journal of Chromatography A 2004;1040(2):193-204.

15. Wu SH,Sun CR, Cao XJ, Zhou H, Zhang H, Pan YJ. Preparative counter-current chromatography isolation of liensinine and its analogues from embryo of the seed of Nel umbo nucifera GAERTN. using upright coil planet centrifuge with four multilayer coils connected in series. Journal of Chromatography A 2004;1041(1-2):153-62.