王金

王金：男,中國科學院上海生命科學研究院副研究員.

微生物生理與合成生物學

1，放線菌氮代謝調控的分子機制；

2，合成生物學使能技術的開發。

1, Jin Wang and Guoping Zhao*. (2009) GlnR positively regulates nasA transcription in Streptomyces coelicolor. Biochemical and Biophysical Research Communications 386 (1): 77-81.

2, Wei Zhao#, Yi Zhong#, Hua Yuan#, Jin Wang#, et al. (2010) Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterranei U32 revealed its genetic characteristics in phylogeny and metabolism. Cell Research 20(10):1096-1108.

3, Ying Wang, XuFeng Cen, GuoPing Zhao* and Jin Wang* (2012) Characterization of a New GlnR Binding Box in the Promoter of amtB in Streptomyces coelicolor Inferred a PhoP/GlnR Competitive Binding Mechanism for Transcriptional Regulation of amtB. Journal of Bacteriology 194 (19): 5237-5244.

4, WeiHua Chen, ZhongJun Qin, Jin Wang* and GuoPing Zhao* (2013) The MASTER (Methylation-Assisted Tailorable Ends Rational) Ligation method for seamless DNA assembly. Nucleic Acids Research DOI: 10.1093/nar/gkt122.

5, Ying Wang, JingZhi Wang, ZhiHui Shao, Hua Yuan, YinHua Lu, WeiHong Jiang, GuoPing Zhao*, Jin Wang* (2013) Three of Four GlnR Binding Sites Are Essential for GlnR-Mediated Activation of Transcription of the Amycolatopsis mediterranei nas Operon. Journal of Bacteriology 195(11): 2595-2602.

6, Ying Wang#, Chen Li#, Na Duan#, Bing Li, XiaoMing Ding, YuFeng Yao, Jun Hu*, GuoPing Zhao*, Jin Wang*. (2014) GlnR Negatively Regulates the Transcription of the Alanine Dehydrogenase Encoding Gene ald in Amycolatopsis mediterranei U32 under Nitrogen Limited Conditions via Specific Binding to Its Major Transcription Initiation Site. PLoS One 9(8): e104811.

7, JiaKun Liu, WeiHua Chen, ShuangXi Ren, GuoPing Zhao*, Jin Wang*. (2014) iBrick: a new standard for iterative assembly of biological parts with homing endonucleases. PLoS One 9(10): e11.

8, Jin Wang*, Ying Wang, GuoPing Zhao (2015) Precise characterization of GlnR Box in actinomycetes. Biochemical and Biophysical Research Communications 458(3): 605-607.

9, ZhuiHui Shao, ShuangXi Ren, XinQiang Liu, Jian Xu, Han Yan, GuoPing Zhao, Jin Wang* (2015) A preliminary study of the mechanism of nitrate-stimulated remarkable increase of rifamycin production in Amycolatopsis mediterranei U32 by RNA-seq. Microbial Cell Factories 14: 75.

10, ZhiHui Shao, WanXin Deng, ShiYuan Li, JuanMei He, ShuangXi Ren, WeiRen Huang, YinHua Lu, GuoPing Zhao, ZhiMing Cai*, Jin Wang* (2015) GlnR-Mediated Regulation of ectABCD Transcription Expands the Role of the GlnR Regulon to Osmotic Stress Management. Journal of Bacteriology 197(19): 3041–3047.

11, XuFeng Cen, JingZhi Wang, GuoPing Zhao, Ying Wang*, Jin Wang* (2016) Molecular evidence for the coordination of nitrogen and carbon metabolisms, revealed by a study on the transcriptional regulation of the agl3EFG operon that encodes a putative carbohydrate transporter in Streptomyces coelicolor. Biochemical and Biophysical Research Communications 471(4):510-514.

12, ShiYuan Li, GuoPing Zhao*, Jin Wang* (2016) C-Brick: A New Standard for Assembly of Biological Parts Using Cpf1. ACS Synthetic Biology DOI: 10.1021/acssynbio.6b00114.

13, Chao Lei#, ShiYuan Li#, JiaKun Liu#, Xuan Zheng, GuoPing Zhao, Jin Wang* (2017) The CCTL (Cpf1-assisted Cutting and Taq DNA ligase-assisted Ligation) method for efficient editing of large DNA constructs in vitro. Nucleic Acids Research DOI: 10.1093/nar/gkx018.

14, Chen Li#, XinQiang Liu#, Chao Lei, Han Yan, ZhiHui Shao, Ying Wang, GuoPing Zhao, Jin Wang*, XiaoMing Ding* (2017) RifZ (AMED_0655) is a pathway-specific regulator for rifamycin biosynthesis in Amycolatopsis mediterranei. Applied Environmental Microbiology. DOI: 10.1128/AEM.03201-16.

15, Xuan Zheng#, ShiYuan Li#, GuoPing Zhao, Jin Wang* (2017) An efficient system for deletion of large DNA fragments in Escherichia coli via introduction of both Cas9 and the non-homologous end joining system from Mycobacterium smegmatis. Biochemical and Biophysical Research Communications. DOI: 10.1016/j.bbrc.2017.02.129

16, ShiYuan Li, GuoPing Zhao, Jin Wang* (2017) Protocols for C-Brick DNA standard assembly using Cpf1. JoVE. doi:10.3791/55775

17, Hong Zhang, Qiuxiang Cheng, Aimin Liu*, Guoping Zhao, Jin Wang* (2017) A novel and efficient method for bacteria genome editing employing both CRISPR/Cas9 and an antibiotic resistance cassette. Frontiers in Microbiology. doi:10.3389/fmicb.2017.00812

18, Xiaochun Zhang#, Jingman Wang#, Qiuxiang Cheng#, Xuan Zheng, Guoping Zhao, Jin Wang* (2017) Multiplex gene regulation by CRISPR–ddCpf1. Cell Discovery 3: 17018; doi: 10.1038/celldisc.2017.18