劉俊

1、植物抗病基因的克隆和功能分析

2、病菌入侵和植物蛋白質組學

植物病原菌的特徵決定了植物對其響應(先天免疫)主要發生在兩個層面上。一個是對病菌表面保守的分子特徵(Pathogen Associated Molecular Patterns，PAMPs)的識別，如細菌的鞭毛蛋白Flagella和伸長因子EF-Tu。這個層面的識別可以啟動基於細胞膜上的受體或受體似蛋白的信號傳遞，從而激活抗性基因的表達（PAMPs triggered immunity, PTI）。另一個層面上是對病菌內的致病因子（effector）的識別。這個層面的識別會激活更強烈的反應，典型的反應是細胞編程性死亡（或稱“超敏反應”）。這一類的反應主要是抗性基因(R基因)的啟動，稱之為ETI （Effector triggered immunity, ETI）。

在眾多植物先天免疫模型中，植物膜蛋白RIN4和病菌的分子互作機制被研究最為透徹。在近期的研究中，我們發現了植物受體似激酶家族VII的一個蛋白RIPK可以特異性地磷酸化RIN4。磷酸化的RIN4可以被抗性基因RPM1識別從而啟動ETI。植物受體似激酶VII是一個大家族，由於其參與了植物先天免疫，我們將用酵母雙雜交和免疫共沉澱的辦法尋找這些植物中同源基因作用的蛋白，分析其在植物先天免疫中的作用。

由於病菌和植物互作主要發生在細胞質膜上，對病菌入侵信號識別的受體和受體似蛋白大多分佈在細胞質膜上，因此對膜蛋白及其結合蛋白的研究具有重要的意義。我們現有的技術可以獲得非常純的細胞質膜，結合先進的質譜技術，使得我們能夠鑒定和分析病菌侵染后膜蛋白組學的變化。通過對膜蛋白質組學的研究，揭示膜蛋白及其作用蛋白在植物抗病中的意義和機理。

Ø 1995年9月-1999 年7月，南京農業大學園藝學院，藥用 植物專業，本科；

Ø 1999年9月-2004年7月，南京農業大學生命科學院，植物學專業，博士；

2004年8月-2006年12月：中科院遺傳發育所博士后

2007年1月-2007年6月：加州大學舊金山分校博士后

2007年6月-2010年2月：加州大學戴維斯分校博士后

2010年2月-2012年3月：加州大學戴維斯分校項目科學家

2012年2月-至今：中科院微生物所研究員，“百人”計劃

國際植物和微生物分子互作學會（MPMI）會員， Plant Physiology, PloS One和Plant Science等雜誌審稿人

Jidong Cao, Chao Yang, Lingjuan Li, Lan Jiang, Chuanwan Wu, Qingyun bu, Guixian Xia, Xiaoyun Liu, Yuanming Luo* and Jun Liu*. Rice plasma membrane proteomics revealsMagnaporthe oryzaepromotes susceptibility by sequential activation of host hormone signaling pathways.Molecular Plant Microbe Interaction. 2016 (Accepted and in press)

Yang C, Li W, Cao J, Meng F, Yu Y, Huang J, Jiang L, Liu M, Zhang Z, Chen X, Miyamoto K, Yamane H, Zhang J, Chen S, Liu J*. Activation of ethylene signaling pathways enhances disease resistance by regulating ROS and phytoalexin production in rice.The Plant Journal,2016 Oct 4. doi: 10.1111/tpj.13388.

Henry E, Fung N, Liu J, Drakakaki G, Coaker G. Beyond glycolysis: GAPDHs are multi-functional enzymes involved in regulation of ROS, autophagy, and plant immune responses.PLoS Genet,2015 Apr 28;11(4):e1005199

Hurley B, Lee D, Mott A, Wilton M, Liu J, Liu YC, Angers S, Coaker G, Guttman DS, Desveaux D. The Pseudomonas syringae type III effector HopF2 suppresses Arabidopsis stomatal immunity.PLoS One, 2014 Dec 11;9(12):e114921

James Elmore, Jun Liu, Barrett Smith, Brett Phinney and Gitta Coaker. Quantitative proteomics reveals dynamic changes in the plasma membrane proteome during Arabidopsis immune signaling.Molecular & Cellular Proteomics.2012, M111.014555

Jun Liu, James Elmore, Daniel Lin and Gitta Coaker. A receptor like cytoplasmic protein kinase phosphorylates RIN4, triggerring plant innate immunity.Cell Host &Microbe, 2011,9, 137–146

Jun Liu#, James Elmore# and Gitta Coaker. Investigating the functions of the RIN4 protein complex during plant innate immune responses.Plant Signaling & Behavior4:12, 1-4.

Tao Chen#, Jun Liu#, Gang Lei, Yun-Feng Liu, Zhi-Gang Li, Jian-Jun Tao, Yu-Jun Hao, Yang-Rong Cao, Qing Lin, Wan-Ke Zhang, Biao Ma, Shou-Yi Chen and Jin-Song Zhang. Effects of Tobacco Ethylene Receptor Mutations on Receptor Kinase Activity, Plant Growth and Stress Responses.Plant and CellPhysiology.2009 50(9):1636-1650 (*: equal contribution)

Jun Liu, James Elmore, Anja Fuglsang, Michael Palmgren. Brian Staskawicz and Gitta Coaker. RIN4 Functions with Plasma Membrane H+-ATPases to Regulate Stomatal Apertures during Pathogen Attack.PLoS Biology. 2009, 7(6): e1000139.

1.

Jidong Cao, Chao Yang, Lingjuan Li, Lan Jiang, Chuanwan Wu, Qingyun bu, Guixian Xia, Xiaoyun Liu, Yuanming Luo* and Jun Liu*. Rice plasma membrane proteomics revealsMagnaporthe oryzaepromotes susceptibility by sequential activation of host hormone signaling pathways.Molecular Plant Microbe Interaction. 2016 (Accepted and in press)

2.

Yang C, Li W, Cao J, Meng F, Yu Y, Huang J, Jiang L, Liu M, Zhang Z, Chen X, Miyamoto K, Yamane H, Zhang J, Chen S, Liu J*. Activation of ethylene signaling pathways enhances disease resistance by regulating ROS and phytoalexin production in rice.The Plant Journal,2016 Oct 4. doi: 10.1111/tpj.13388.

3.

Henry E, Fung N, Liu J, Drakakaki G, Coaker G. Beyond glycolysis: GAPDHs are multi-functional enzymes involved in regulation of ROS, autophagy, and plant immune responses.PLoS Genet,2015 Apr 28;11(4):e1005199

4.

Hurley B, Lee D, Mott A, Wilton M, Liu J, Liu YC, Angers S, Coaker G, Guttman DS, Desveaux D. The Pseudomonas syringae type III effector HopF2 suppresses Arabidopsis stomatal immunity.PLoS One, 2014 Dec 11;9(12):e114921

5.

James Elmore, Jun Liu, Barrett Smith, Brett Phinney and Gitta Coaker. Quantitative proteomics reveals dynamic changes in the plasma membrane proteome during Arabidopsis immune signaling.Molecular & Cellular Proteomics.2012, M111.014555

6.

Jun Liu, James Elmore, Daniel Lin and Gitta Coaker. A receptor like cytoplasmic protein kinase phosphorylates RIN4, triggerring plant innate immunity.Cell Host &Microbe, 2011,9, 137–146

7.

Jun Liu#, James Elmore# and Gitta Coaker. Investigating the functions of the RIN4 protein complex during plant innate immune responses.Plant Signaling & Behavior4:12, 1-4.

8.

Tao Chen#, Jun Liu#, Gang Lei, Yun-Feng Liu, Zhi-Gang Li, Jian-Jun Tao, Yu-Jun Hao, Yang-Rong Cao, Qing Lin, Wan-Ke Zhang, Biao Ma, Shou-Yi Chen and Jin-Song Zhang. Effects of Tobacco Ethylene Receptor Mutations on Receptor Kinase Activity, Plant Growth and Stress Responses.Plant and CellPhysiology.2009 50(9):1636-1650 (*: equal contribution)

9.

Jun Liu, James Elmore, Anja Fuglsang, Michael Palmgren. Brian Staskawicz and Gitta Coaker. RIN4 Functions with Plasma Membrane H+-ATPases to Regulate Stomatal Apertures during Pathogen Attack.PLoS Biology. 2009, 7(6): e1000139.