李國梁

2003.09－2007.07青島農業大學，獲得學士學位。

2007.09－2010.07中國科學院西北高原生物研究所，獲得碩士學位。

2010.09－2012.07中國科學院西北高原生物研究所，獲得博士學位。

2012.07－ 今 在曲阜師範大學山東省綠色天然產物及醫藥中間體開發重點實驗室、山東省生命有機分析重點實驗室從事教學與科研工作.，主要講授本科生課程《天然藥物化學》，《天然產物與新葯開發》及研究生課程《生物化學》。

食品分析方向：設計併合成高靈敏度熒光探針分子，結合改性劑用於高效液相色譜中低含量化合物的熒光識別，並結合HPLC-MS 或CE 實現了對食品中毒性成分及功能成分的高靈敏、高選擇性檢測。

天然產物中活性成分研究：主要從事葯食同源食品中活性成分分離、純化及鑒定，並利用建立方法進行活性成分進行精確定量，對得到的活性單體進行體外活性研究。

目前已發表研究論文40餘篇，其中SCI收錄36 篇，申請國家發明專利2項。主持國家級、省級、校級科研項目4項，參與國家自然基金項目5項，省部級項目3項。

目前已發表研究論文40餘篇，其中SCI收錄38 篇，申請國家發明專利3項。擔任國際學術雜誌《Current Nutrition & Food Science》, 《Journal of Food, Nutrition and Dietetics》及《Journal of Experimental Food Chemistry》編委。

同時擔任《Biosensors and Bioelectronics》；《Journal of Agricultural and Food Chemistry》；《Food Research International》；《Food Chemistry》；《Food & Function》；《Journal of Separation Science》；《Talanta》；《Biomedical Chromatography》；《Analytical method》；《RSC Advances》等SCI雜誌審稿專家。

科研項目

1，石榴皮中三萜酸成分及其降糖活性研究；2014年1月-2016年12月，國家自然基金委青年基金項目，主持，進行中（NO 31301595）

2，藏葯獐牙菜中三萜酸的準確分析及其指紋圖譜建立；2010年1月-2012年12月：中國科學院科技創新類項目，主持，結題，（NO36）

3，石榴皮中三萜酸成分及其降血糖活性研究；2014年1月-2016年12月，曲阜師範大學科技計劃項目，主持，進行中（編號xkj201302）,

4，基於柱前熒游標記—分散液液微萃取技術的水產品中生物胺快速、高靈敏HPLC檢測方法研究及其應用，2014年,6月-2016年12月；廣東省水產品加工與安全重點實驗室資助，主持，進行中（NO：GDPKLAPPS1401）

5,基於宏基因組文庫的新殼聚糖酶篩選及計算機輔助合理化設計；2014年1月-2016年12月，國家自然基金委項目, 排名第二, 進行中（NO 31301438）

近年發表論文情況：(*為通訊作者)

[1] Guoliang Li*,Weiheng Kong,Meizhao, et al, A fluorescence resonance energy transfer (FRET) based “Turn-On” nanofluorescence sensor using a nitrogen-doped carbon dot-hexagonal cobalt oxyhydroxidenanosheet architecture and application to α-glucosidase inhibitor screening， Biosensors and Bioelectronics ,2016. doi:10.1016/j.bios.2015.12.094 (IF=6.48)

[2] Guoliang Li*, Huili Fu, Xuejie Chen, et al, Facile and Sensitive Fluorescence Sensing of Alkaline Phosphatase in Vitro and Living Cells with Highly Photoluminescent Carbon Dots Based on Inner Filter Effect, Analytical Chemistry, 2016, DOI: 10.1021/acs.analchem.5b04193 (IF=5.636)

[3] Guoliang Li,Jinmao You, Cuihua Song, YouruiSuo, Development of a New HPLC Method with Precolumn Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits, Journal of Agricultural and Food Chemistry, 2011,59 (7), 2972–2979. (SCI 收錄)

[4] Guoliang Li,Jinmao You, YouruiSuo, Cuihua Song, Zhiwei Sun, A developed pre-column derivatization method for the determination of free fatty acids in edible oils by reversed-phase HPLC with fluorescence detection and its application to Lyciumbarbarum seed oil, Food Chemistry, 2011，125(4) 1365-1372. (SCI 收錄)

[5] Guoliang Li,Yanyan Cui, Jinmao You, Yourui Suo, Determination of trace amino acids by a selective and sensitive pre-column derivatization method using HPLC-FLD-MS/MS, Amino acids,2011,40(4), 1185-1193. (SCI 收錄)

[6] Guoliang Li,Xiaolong Zhang, Jinmao You, Zhiwei Sun, YouruiSuo, Highly sensitive and selective pre-column derivatization high-performance liquid chromatography approach for rapid determination of triterpenes acids and application to Swertia species, AnalyticaChimicaActa, 2011, 688(2)208-218. (SCI 收錄)

[7] Guoliang Li,Junyou Shi,Zhiwei Sun, You Jinmao,Yongjun Liu, Supercritical CO cell breaking extraction of Lyciumbarbarum seed oil and determination of its chemical composition by HPLC/APCI/MS and antioxidant activity. LWT-Food Science and Technology,2011,44(4), 1172-1178. (SCI 收錄)

[8] Li GL， Dong, L., Wang, A., Wang, W., Hu, N., & You, J.. Simultaneous determination of biogenic amines and estrogens in foodstuff by an improved HPLC method combining with fluorescence labeling. LWT-Food Science and Technology, 2014,55(1), 355-361. (SCI 收錄)

[9] Guoliang Li, Zhiwei Sun, Jinmao You, Supercritical CO oil extraction from Chinese star anise seed and simultaneous compositional analysis using HPLC by fluorescence detection and online atmospheric CI-MS identification. Journal of the Science of Food and Agriculture,2010, 90(11):1905-1913. (SCI 收錄)

[10] Guoliang Li, Sun, Zhiwei, Xia, Lian,Jinmao You, Optimization of red pepper seed oil extraction using supercritical CO and analysis of the composition by reversed-phase HPLC-FLD-MS/MS. International Journal of Food Science & Technology, 2011, 46(1):44-51. (SCI 收錄)

[11] Guoliang Li,Guang Chen, Liu, Jinmao You. A sensitive and selective hplc-fld method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum [J]. Journal of Chromatography B, 2012(859)191-195 (SCI 收錄)

[12] Guoliang Li, Zhiwei Sun, Cuihua Song, Jinmao You. A sensitive fluorescence reagent for amino acids determination in Saussurea involucrate and Artemisia capillarisThunb using high‐performance liquid chromatography with fluorescence detection and identification with mass spectroscopy/electrospray ionization source Biomed. Chromatogr. 2011:6(689–696). (SCI 收錄)

[13] Guoliang Li,Cuihua Song, Jinmao You, Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD[J], Journal of the American Oil Chemists' Society, 2012,89(4) 585-595 (SCI 收錄)

[14] Hongliang Wu, Guoliang Li* , Shucheng Liu, ZhongyinJia, Qiulong Zhang and Jinmao You*; Simultaneous determination of seven biogenic amines in foodstuff samples using one-step fluorescence labeling and dispersive liquid-liquid microextraction followed by HPLC-FLD and method optimization using response surface methodology[J], Food Analytical Methods, DOI: 10.1007/s12161-014-9943-2 (SCI 收錄)

[15] Wenli Wang, Guoliang Li*,ZhongyinJi, Na Hu, Jinmao You*,A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification[J], Food Analytical Methods, 2014, 7(7): 1546-1556 (SCI 收錄)

[16] Guoliang Li*, Shucheng Liu, Zhiwei Sun, Lian Xia, Guang Chen, Jinmao You*, A simple and sensitive HPLC method based on precolumn fluorescence labeling for multiple classes of plant growth regulator determination in food samples[J], Food Chemistry,. 2015, 170: 123-130. (SCI 收錄)

[17] Aihong Wang, Guoliang Li*, Jinmao You*, A new fluorescent derivatization reagent and its application to free fatty acid analysis in pomegranate samples using HPLC with fluorescence detection [J], Journal of Separation Science, 2013,36(24):3853-9 (SCI 收錄)

[18] Hongliang Wu, Guoliang Li*, Shucheng Liu and Jinmao You* Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection[J], Journal of Pharmaceutical and Biomedical Analysis, doi:10.1016/j.jpba.2014.10.031 (SCI 收錄)

[19] Li G*, Lu S, Wu H, Chen G, You J*. Determination of multiple phytohormones in fruits by high‐performance liquid chromatography with fluorescence detection using dispersive liquid–liquid microextraction followed by pre‐column fluorescent labeling[J]. Journal of separation science, DOI: 10.1002/jssc.201401131 (SCI 收錄)

[20] Guoliang Li*, Chenhong Tang, Jinmao You*,et al, A rapid and sensitive method for semicarbazide screening in foodstuffs by HPLC with fluorescence detection[J], Food Analytical Methods,DOI: 10.1007/s12161-014-0063-9 (SCI 收錄)

[21] Hongliang Wu, Guoliang Li*,Monitoring the contents of six steroidal and phenolic endocrine disrupting chemicals in chicken, fish and aquaculture pond water samples using pre-column derivatization and dispersive liquid–liquid microextraction with the aid of experimental design methodology, Food Chemistry,doi:10.1016/j.foodchem.2015.06.059(SCI 收錄)

[22] Guoliang Li*, Chenhong Tang, Jinmao You，Rapid and sensitive screening of some acidic micronutrients in infant foods by HPLC with fluorescent detector， Journal of the Science of Food and Agriculture，DOI: 10.1002/jsfa.7457(SCI 收錄)

[23] Cuihua Song, Caiqing Zhang, Guoliang Li， Highly selective and sensitive determination of free and total amino acids in apocynumvenetum l.(luobuma tea) by a developed hplc-fld method coupled with pre-column fluorescent labelling [J]. International Journal of Food Sciences and Nutrition,2012,63:170-177 (SCI 收錄)

[24] Junyou Shi, Guoliang Li, Rui Zhang, JieZheng, YouruiSuo, A VALIDATED HPLC-DAD METHOD FOR IDENTIFYING AND DETERMINING THE BIOACTIVE COMPONENTS OF TWO KINDS OF LUOBUMA, 2011, 34(7), 537-547.(SCI 收錄)

[25] YinfengXie, Guoliang Li, Jinmao You, A Novel Labeling Reagent of 2-(12-Benzo[b]acridin-5-(12H)-yl)- acetohydrazide for Determination of Saturated and Unsaturated Fatty Acids in Traditional Chinese Herbs by HPLC-APCI-MS, Chromatograpia, DOI: 10.1007/s10337-012-2226-4 (SCI 收錄)

[26] Junyou Shi, Guoliang Li, Honglun Wang, JieZheng, YouruiSuo, One‐step Separation of Three Flavonoids from Poacynumhendersonii by High‐speed Counte current Chromatography, Phytochemical analysis, 2011,22(5),450-454. (SCI 收錄)

[27] Jing Nianhua., Shi Junyou, Li Guoliang, Jinmao You. Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry [J]. Food Research International, 2012,48(1) 155–163( SCI 收錄)

[28] Chen, G., Li, J., Sun, Z., Zhang, S., Li, G.L, Song, C., Suo, Y., & You, J. (2014). Rapid and sensitive ultrasonic-assisted derivatisationmicroextraction (UDME) technique for bitter taste-free amino acids (FAA) study by HPLC–FLD. Food Chemistry, 143, 97-105 (SCI 收錄)

[29] Chen, G., Li, J., Zhang, S., Song, C., Li, G.L, Sun, Z., Suo, Y., & You, J. (2012). A sensitive and efficient method to systematically detect two biophenols in medicinal herb, herbal products and rat plasma based on thorough study of derivatization and its convenient application to pharmacokinetics with semi-automated device. Journal Of Chromatography A, 1249, 190-200. (SCI 收錄)

[30] Nianhua Jing， Guoliang Li, Jinmao You, QUANTITATIVE ANALYSIS OF FATTY ACIDS FROM SNOWLOTUS (SAUSSUREA) SPECIES USING HPLC WITH FLUORESCENCE DETECTION AND ATMOSPHERIC CHEMICAL IONIZATION-MASS SPECTROMETRY, Journal of Liquid Chromatography & Related Technologies, 2012, (13)1882-1894 (SCI 收錄)

[31] Lian Xia, Jinmao You, Guoliang Li,ZhiweiSun,YouruiSuo, Compositional and Antioxidant Activity Analysis of Zanthoxylumbungeanum Seed Oil Obtained by Supercritical CO2 Fluid Extraction, Journal of the American Oil Chemists' Society, 2011 (88)23–32. (SCI 收錄)

[32] Lian Xia, Zhi Wei Sun, Guo Liang Li, You Rui Suo, A sensitive analytical method for the component monosaccharides of the polysaccharides from a Tibetan herb Potentilla anserine L. by capillary zone electrophoresis with UV detector, European Food Research and Technology, 2010,230, (5), 715-722 (SCI 收錄)