楊麗

2003.09-2004.10愛爾蘭University College Cork,博士后。

2005.4-2006.7美國路易斯安那州立大學，博士后。

2006.7-2007.12美國馬里蘭大學，Calibrant Biosystem公司，博士后。

2008.2-2014.2東北師範大學，化學學院，副教授，博士生導師

現任東北師範大學化學學院副教授。

主要研究方向為毛細管電泳/電色譜技術在生化分析、多維分離技術聯用以及納米材料科學中的基礎和應用研究。

科研項目

吉林省科技廳重點研發項目，“新型全自動在線藥物溶出分析檢測技術及其儀器研發”、2020.01-2022.12、30萬 元、在研、主持。國家自然科學基金面上項目，21775017、“口服固體藥物製劑體外溶出度的高時間分辨在線過程分析”、2018.01-2021.12、65萬元、在研、主持。國家自然科學基金面上項目，21475019、“基於毛細管電泳技術的微生物胞內目標代謝物在線分析”、2015/01-2018/12、80萬元、已結題、主持。國家自然科學基金面上項目，21175018、“以毛細管電泳為基礎的酶切多肽反應全程的高時間分辨檢測技術及應用研究”、2012/01-2015/12、56萬元、已結題、主持。

吉林省科技廳重點項目，20120431、“基於液液微萃取-高效毛細管電泳技術聯用的生物檢材中痕量濫用藥物的快速靈敏檢測”、2012/01-2014/12、15萬元、已結題、主持。東北師範大學“東師學者”青年學術骨幹培養計劃，2012/01-2014/12、10萬元、已結題、主持。國家自然科學基金青年項目、20805005、“光快門漂白進樣-電泳媒介微分析法連用的毛細管電泳在線酶反應研究平台”、2009/01-2011/12、20萬元、已結題、主持。教育部新教師基金、200802001014、“開管毛細管固定化酶反應器與雙酶底物循環法聯用的毛細管電泳谷氨酸測定平台”、2009/01-2011/12、3.6萬元、已結題、主持。教育部留學回國基金、“利用開管毛細管固定化酶反應器的毛細管電泳法測定痕量谷氨酸”、2009/01-2011/12、3.0萬元、已結題、主持。吉林省科技廳青年基金、20080118、“開管毛細管電色譜測定痕量谷氨酸”、2008/10-2010/10、6萬元、已結題、主持。國家“863”計劃課題、2013AA032204、“面向公共安全用納米材料及診斷技術的研發”、2013/12-2016/12、1234萬元、在研、課題骨幹。國家科技支撐計劃項目子課題、2012BAK02B04、“物證檢驗與溯源關鍵技術與裝備研發”、2012/2-2014/12、525萬元、在研、課題骨幹。

主要研究論文

2020年Immunoassays using optical-fiber sensor with all-directional chemiluminescent collection. Anal. Chem. 2020,92,6257−6262 A portable pencil-like immunosensor for point-of-care testing of inflammatory biomarkers. Anal. Bioanal. Chem. 2020,412,3231-3239 Ultrasensitive determination of organotin compounds in plastic food packaging and edible oils by sheathless capillary electrophoresis-electrospray ionization-mass spectrometry. Analyst 2020,145,2286 Capillary electrophoresis-integrated immobilized enzyme microreactor with graphene oxide as support: Immobilization of negatively charged L-lactate dehydrogenase via hydrophobic interactions. Electrophoresis 2020,41,175-182 模塊化自組裝高效毛細管電泳教學儀的研製及實驗教學應用，化學教育，2020,41,36-40 Capillary electrophoresis-immobilized enzyme microreactors for acetylcholinesterase assay with surface modification by highly-homogeneous microporous layer. J. Chromatogr. A 2020,1609,460454

2019年Ultra-sensitive capillary immunosensor combining porous-layer surface modification and biotin streptavidin nano-complex signal amplification: Application for sensing of procalcitonin in serum. Talanta 2019,205,120089 Enzyme-like catalysis of polyoxometalates for chemiluminescence: Application in ultrasensitive detection of H2O2 and blood glucose. Talanta 2019,205,120139 Automatic dissolution testing with high-temporal resolution for both immediate-release and fixed-combination drug tablets. Sci.Rep. 2019,205,120139 Sensitive detection of benzophenone-type ultraviolet filters in plastic food packaging materials by sheathless capillary electrophoresis-electrospray ionization-tandem mass spectrometry. J. Chromatogr. A 2019,1604,460469 Optical fiber-mediated immunosensor with a tunable detection range for multiplexed analysis of veterinary drug residues. ACS Sensors 2019,4,1864-1872 A highly sensitive capillary-based immunosensor by combining with peroxidase nano complex-mediated signal amplification for detection of procalcitonin in human serum. ACS Omega 2019,4,6210-6217 Converting solution viscosity to distance-readout on paper substrates based on enzyme-mediated alginate hydrogelation: Quantitative determination of organophosphorus pesticides. Anal. Chim. Acta 2019,1071,1-7 Surface modification with highly-homogeneous porous silica layer for enzyme immobilization in capillary enzyme microreactors. Talanta 2019,197,539-547

2018年Capillary electrophoresis-integrated immobilized enzyme microreactor utilizing single-step in-situ penicillinase-mediated alginate hydrogelation: Application for enzyme assays of penicillinase. Talanta 2018,189,377-382 Multiplex immunoassays using surface modification-mediated porous layer open tubular capillary. Anal. Chim. Acta 2018,1043,1-10 Enzyme assay for d-amino acid oxidase using optically gated capillary electrophoresis-laser induced fluorescence detection. J. Chromatogr. A 2018,1548,83-91 Single-step in situ acetylcholinesterase-mediated alginate hydrogelation for enzyme encapsulation in CE. Anal. Chem. 2018,90,4071-4078 Rapid and sensitive tapered-capillary microextraction combined to on-line sample stacking-capillary electrophoresis for extraction and quantification of two beta-blockers in human urine. Talanta 2018,180,90-97 Highly uniform porous silica layer open-tubular capillary columns produced via in-situ biphasic sol-gel processing for open-tubular capillary electrochromatography. J. Chromatogr. A 2018,1538,86-93

2017年Photonic crystal fiber-based immunosensor for high-performance detection of alpha fetoprotein. Biosens. Bioelectron. 2017,91,431-435 Screening platform based on robolid microplate for immobilized enzyme-based assays. ACS Omega 2017,2,5079-5086 Core-shell silica microsphere-based trypsin nanoreactor for low molecular-weight proteome analysis. Anal. Chim. Acta 2017,985,194-201

2016年 Facile synthesis of electrospinning Mn2O3-Fe2O3 loaded carbon fibers for electrocatalysis of hydrogen peroxide reduction and hydrazine oxidation. Electrochim. Acta. 2016,211,255-264 Capillary zone electrophoresis as a tool to monitor the stability and hydrolytic equilibria of strandberg-type polyoxometalates in aqueous solution. Eur. J Inorg. Chem. 2016,4179-4184 Enzyme and inhibition assay of urease by continuous monitoring of the ammonium formation based on capillary electrophoresis. Electrophoresis 2016,37,2692-2698 Sequential on-line C-terminal sequencing of peptides based on carboxypeptidase Y digestion and optically gated capillary electrophoresis with laser-induced fluorescence detection. J. Chromatogr. A 2016,1459,152-159 Capillary electrophoresis-integrated immobilized enzyme reactors. Rev. Anal. Chem. 2016,35,115-131 Determination of the inhibitory effect of green tea extract on glucose-6-phosphate dehydrogenase based on multilayer capillary enzyme microreactor. Biomed. Chromatogr. 2016,30,1210-1215

2015年Sequential capillary electrophoresis analysis using optically gated sample injection and UV/vis detection. Electrophoresis 2015,36,2380-2385 Enhancing separation in short-capillary electrophoresis via pressure-driven backflow. Electrophoresis 2015,36,1549-1554 On-plate enzyme and inhibition assay of glucose-6-phosphate dehydrogenase using thin-layer chromatography. J. Sep. Sci. 2015,38,2753-2930 Application of Thin-Layer Chromatography in Enzyme Activity and Inhibitors Studies of Glucose-6-Phosphate Dehydrogenase. JPC-J. Planar Chromat. 2015,28,333-336 Application of capillary enzyme micro-reactor in enzyme activity and inhibitors studies of glucose-6-phosphate dehydrogenase. J. Chromatogr. B 2015,990,174-180 Theoretical and experimental studies on sequential two-diffusional sample injection for capillary electrophoresis. J. Chromatogr. A 2015,138,247-252

2014年Inhibition study of alanine aminotransferase enzyme using sequential online capillary electrophoresis analysis. Anal. Biochem. 2014,467,28-30 Capillary electrophoresis-based immobilized enzyme reactor using particle-packing technique. J. Chromatogr. A 2014,1352,80-86 A capillary electrophoresis-based immobilized enzyme reactor using graphene oxide as a support via layer by layer electrostatic assembly. Analyst 2014,139,1973 Efficient capillary electrophoresis separation and determination of free amino acids in beer samples. Electrophoresis 2014,35,577-584 Sequential micellar electrokinetic chromatography analysis of racemization reaction of alanine enantiomers. J. Chromatogr. A 2014,1331,123-12

2013年Ionic liquid-based dispersive liquid-liquid microextraction coupled with capillary electrophoresis to determine drugs of aAbuse in urine. Chinese J. Anal. Chem. 2013,41,1919-1922 Graphene oxide and reduced graphene oxide as novel stationary phases via electrostatic assembly for open-tubular capillary electrochromatography. Electrophoresis 2013,34,1869-1876 Application of cyclodextrin-modified gold nanoparticles in enantioselective monolith capillary electrochromatography. Talanta 2013,109,1-6 Application of graphene as the stationary phase for open-tubular capillary electrochromatography.J. Chromatogr. A 2013,1277,93-97

2012年Electrochemistry and simultaneous detection of metabolites of purine nucleotide based on large mesoporous carbon modified electrode. Electroanal. 2012,24,1401-1408 Online enzyme discrimination and determination of substrate enantiomers based on electrophoretically mediated microanalysis. Anal. Chem. 2012,84,6701-6706 Method for the sequential online analysis of enzyme reactions based on capillary electrophoresis. Anal. Chem. 2012,84,2961-2967 A replaceable dual-enzyme capillary microreactor using magnetic beads and its application for simultaneous detection of acetaldehyde and pyruvate. Electrophoresis 2012,33,2145-2151

2011年Enantioselective open-tubular capillary electrochromatography using cyclodextrin-modified gold nanoparticles as stationary phase. J. Chromatogr. A 2011,1218,3725-3729 爆炸殘留物的毛細管電泳檢測。分析化學 2011,39,1293-1294

2010年Use of cyclodextrin-modified gold nanoparticles for enantioseparations of drugs and amino acids based on pseudostationary phase-capillary electrochromatography. Electrophoresis 2010,31,1-9 High temporal resolution monitoring of enzyme reaction and inhibition using optically gated vacancy capillary electrophoresis and immobilized enzyme. Anal. Chim. Acta 2010,683,136-142

2009年Dual-enzyme, co-immobilized capillary microreactor combined with substrate recycling for high-sensitive glutamate determination based on CE. Electrophoresis 2009,30,3527-3533

高等分析化學，分析化學，化合物成分分析實驗，Chromatography（留學生研究生課程）。