張華山

張華山（Huashan Zhang），1946年10月31日出生，理學碩士，教授、博士生導師。

(1)化學系和藥學專業本科生的“分析化學”。

(2)分析化學專業碩士研究生“分子探針與檢測試劑”和“稀土材料與分析”。

12名博士和2名碩士研究生（已有4名博士生畢業並獲學位）。

(1)生物分子的近紅外熒游標記試劑的合成、性能及應用研究，國家自然科學基金，8萬，1996.1-1998.12

(2)檢測和造影細胞中一氧化氮的新型熒光探針研究，國家自然科學基金，10萬（2000-2002）。

(3)含二吡咯甲烷-二氟化硼基團的高靈敏生物分子熒光探針研究，教育部博士點基金，5.0萬（2000-2002）。

(4)單個細胞神經遞質釋放的微分析系統研製，武漢科委，6萬（2003-2004）。

(5)預警心血管疾病熒光探針和試劑盒的研究，湖北省科技攻關項目,2萬(2003)

(6)分離熒光檢測代謝物中干擾素的研究，合作項目，10.0萬（2002-2004）。

(1)曾雲鶚，張華山，陳震華，無機離子顯色劑（化學工業出版社，1989）；

(2)王克謙，張華山譯，Caulcutt R.（美）著，《分析工作者用統計學》，科學出版社，1989年；

(3)江祖成，蔡汝秀，張華山，《稀土元素分析化學》（第二版），科學出版社，1999年；

(4)張華山，王紅，趙媛媛，分子探針和檢測試劑，科學出版社，2002，北京。

1.Y.-Y.Zhao,H.Wang,H.-S.Zhang*,G.-Lxu,N-Hydroxysuccinimidyl-3-indolylacetate as a precolumn derivatzing reagent to separate and determine peptides and their hydrolysates by reversed-phase high performance liquid chromatography.J.Liquid Chromatogr. & Rel.Tech.,1998,21(12),1870

2.H.-S.Zhang,J-F.Zhang,H.Wang and X.-Y.Li,Synthesis and analytical application of2,6-dichloroarsenazo as a chromogenic reagent for bismuth.Anal.Chem.Acta,1999,380(1),101.

3.Hong Wang,Hua-shan Zhang*,Jie-ke Cheng, Studies on 2-(2thiazolylazo)-5-diethylaminophenol as a precolumn derivatizing reagent in the separation of platinum group metals by high performance liquid chromatography.Talanta,1999,48(1),1

4.徐國良，王紅，張華山*，N-羥基琥珀醯亞胺-a-萘乙酸酯的合成及其作為柱前衍生試劑在反相高效液相色譜中的應用。化學試劑，1999，21（1），8

5.徐國良，張華山，王紅。新熒光衍生試劑N-羥基琥珀醯亞胺吲哚乙酸酯的合成、性能及應用研究。化學試劑，1999，21(1)，1。

6.Hong Wang, Jin Li, Xin Liu, Ting-Xian Yang and Hua-Shan Zhang. N-hydroxysuccinimidyl fluorescein-O-acetate as a fluorescent derivatizing reagent for catecholamines in liquid chromatography. Anal. Biochem., 2000, 281, 15.

7.Hong Wang, Wei Yang, Shu-Cai Liang, Zhi-Min Zhang and Hua-Shan Zhang. Spectrofluorimetric determination of nitrite with 5, 6-diamino-1, 3-naphthalene disulfonic acid. Anal. Chim. Acta, 2000, 419(2), 169.

8.Hong Wang, Jin Li, Xin Liu and Hua-Shan Zhang. N-Hydroxysuccinimidyl fluorescein-O-acetate as a highly fluorescent derivatizing reagent for aliphatic amines in high performance liquid chromatography. Anal. Chim. Acta, 2000, 423(1), 77.

9.趙媛媛，王紅，張華山。柱前衍生反相高效液相色譜分離和測定氨基酸-用N-羥基琥珀醯亞胺-3-萘乙酸酯作衍生試劑。分析科學學報，2000，16(3)，188。

10.李進，王紅，曹啟花，張華山。某些變色酸偶氮類染料與蛋白質作用的分光光度研究。化學試劑，2000，22(3)，129。

11.Hong Wang, Wei-Sheng Wang and Hua-Shan Zhang. Spectrofluorimetic determination of cysteine based on the fluorescence inhibition of Cd(II)-8-hydroxyquinoline-5-sulphonic acid complex by cysteine. Talanta, 2001, 53, 1015.

12.Xin Liu, Hong Wang, Shu-Cai Liang, Hua-Shan Zhang. 4:3-b-Naphthapyrone-4-acetic acid N-hydroxysuccinimidyl ester as a fluorescent labeling reagent for amino acids and oligopeptides in high performance liquid chromatography. Chromatographia, 2001, 53(5/6), 326-330.

13.Xin Liu, Hong Wang, Shu-Cai Liang, Hua-Shan Zhang. Determination of primary and secondary aliphatic amines by N-hydroxysuccinimidyl 4, 3, 2’-naphthapyrone-4-acetate and reversed-phase high-performance liquid chromatography. Anal. Chim. Acta, 2001, 441, 45-52.

14.Shu-Cai Liang, Hong Wang, Zhi-Min Zhang, Yu-Yang Zhou, Hua-Shan Zhang. Spectrofluorimetic determination of thiols by N-[p-(2-benzoxazolyl)phenyl]maleimide. Fresenius’ J. Anal. Chem., 2001, 370, 1117-1119.

15.Hong Wang, Jin Li, Ting-Xian Yang, and Hua-Shan Zhang, N-Hydroxysuccinimidyl-fluorescein-O-acetate for precolumn fluorescence derivatization of amino acids and oligopeptides in liquid chromatography. J. Chromatogr. Sci., 2001, 39, 365-369.

16.Hong Wang, Wei-Sheng Wang and Hua-Shan Zhang. A spectrofluorimetric method for cysteine and glutathione using the fluorescence system of Zn(II)-8-hydroxyquinoline-5-sulphonic acid complex. Spectrochim. Acta, Part A, 2001, 57, 2403-2407.

17.Hong Wang, Yuan-Yuan Zhao, Hong Jin, Hua-Shan Zhang N-Hydroxysuccinimidyl-a-naphthylacetate as a derivatizing reagent for amino acids and oligopeptides in reversed-phase high performance liquid chromatography, J. Liq. Chromatogr. Rel. Technol., 2001, 24(20), 3157-3170.

18. ZHANG XIAN ,WANG HONG,ZHANG HUA SHAN, Spectrofluorimetric determination of magnesium with 7-(8- hydro xy-3,6-disulfonaphthylazo)-8-hydroxyquinoline-5-sul-fonic acid WUHAN UNIVERSITY JANURUAL OF NATURALSCIENCE,6(4),83(2001),

19.Yuan-Yuan Zhao, Zhi-Zhong Jing, Hua-Shan Zhang*,The determination of impurities in caprolactam by capillary gas chrimatography-mass spectrometry,Microchemical Journal,2001, 69, 213-217

20.Shu-Cai Liang, Hong Wang, Zhi-Min Zhang, Xian Zhang, Hua-Shan Zhang. Direct spectrifluorimetric determination of glutathione in biological samples using 5-malermidyl-2-(m-methylphenyl) benzoxazole. Anal. Chim. Acta, 2002, 451(2), 211-219.

21.Xian Zhang, Hong Wang, Shu-Cai Liang, Hua-Shan Zhang. Spectrofluorimetric determination of nitric oxide at trace levels with 5, 6-diamino-1, 3-naphthalene disulfonic acid. Talanta, 2002, 56(3), 499-504.

22. 黃文勝，王紅，張軍鋒，張華山，三氯偶氮氯膦測定鉍的顯色反應研究, 分析科學學報,18（3），199（2002）

23.黃文勝，王紅，張軍鋒，張華山，新顯色劑2，6－二碘－4－硝基偶氮氯磷光度法測定鉍的研究，分析化學，30(4),470(2002)

24.黃文勝，王紅，張軍鋒，張華山，新顯色劑間氟偶氮氯磷分光光度法測定鉍的研究，冶金分析，22(12),6(2002)

25.黃文勝，王紅，張軍鋒，張華山，三氯偶氮氯膦測定鉍的顯色反應研究，分析科學學報,18（3），（2002）

26.Shu-Cai Liang, Hong Wang ,Zhi-min Zhang,Xian Zhang,Hua-shan Zhang,Spectrofluorimetric determination of cysteine by 5-maleimidyl-2-(m-methylphenyl)benzoxazole, Spectrochimica Acta A,58,2605(2002)

27.Y.-Y.Zhao,Zhi-zhong Jing Hong.Wang,Hua-shan Zhang*, N-Hydroxysuccinimidylphenylacetate as a novel derivatizing reagent for aliphatic amines in gas chromatography,Anal. Chim. Acta, 468,255(2002).

28.Zhen Shen,Hong Wang, Shu-cai Liang,Zhi-Min zhang,and Hua-Shan Zhang,Spectrofluorimetric Determination of Reduced Glutathione in Human Blood Using N-[p-(2-benzothi azoyl)-phenyl]maleimide,Analytical Letters,35,(14),2269(2002)

29.Hua LI, Hong WANG, Jin-he CHEN, Li-hua WANG, Hua-shan ZHANG*, Yun FAN, “Determination of amino acid neurotransmitters in cerebral cortex of rats administered with baicalin prior to cerebral ischemia by capillary electrophoresisllaser-induced fluorescence detection, J. Chromatogr. B, 788,93(2003)

30.J-F Zhang,H.Wang,A-X Hou,C-F Wang,H-S Zhang,Speciacion of thulium porphyrin complexes by reversed-phase HPLC,26(6),911(2003)

31.Xian Zhang,Hong Wang,Jin–shu Li, Hua-shan Zhang,Development of a fluorescent probe for nitric oxide detection based on difluoroboradiaza-s-indacene fluorophore,Anal.Chim.Acta,481,101(2003)

32.Xian Zhang,Hong Wang,Ni-na Fu,Hua-shan Zhang,A fluorescence quenching method for the determination of nitrite with rhodamine 110,Spectrochimica Acta A,59,1667(2003)

33.Li-wei Cao,Xin Liu,Hong Wang,Hua-Shan Zhang.Spectrofluorimetric determination of aliphatic amines using a new fluorigenic reagent:2,6-dimehtylquinoline-4-(N-succinimidyl)-formate,Talanta,56,973(2003).

(1)陳震華，張華山，黎心懿，曾雲鶚，國家發明三等獎（1987）；

(2)程介克，劉錦春，張華山，劉良斌國家教委科技進步獎三等獎（1993年）；

(3)張華山，王紅，黎心懿，程介克，教育部科技進步獎三等獎（1999年）。