蓋宏偉

蓋宏偉，主要從事單分子/單個納米粒子的顯微成像技術研究，已發表SCI論文二十餘篇，其中多數發表在Angewandte Chemie International Edition, Lab on a Chip, Analytical Chemistry, Chemical Communications, Trends in Analytical Chemistry, Journal of Microscopy, Electrophoresis等高影響力的科研期刊上。獲國內發明專利五項。主持國家自然科學基金兩項。已培養了碩士研究生6名。為碩士研究生主講《分子與細胞成像技術》，為本科生主講《生化儀器原理與方法》。

以顯微成像為主要手段，在細胞和單分子層面上，開展以高靈敏檢測和動態監測為特徵的疾病早期診斷和致病機理研究。其中包括生物晶元與單分子檢測技術的耦合，動態單分子間相互作用的監測，納米顯微技術，納米粒子自組裝的動態監測等。

1，國家自然科學基金，20705007，高通量單分子微陣列晶元晶元；

2，湖南省科技廳國際合作項目，2008WK3004，基於單分子微陣列晶元的肺癌骨髓微轉移研究；

3，國家自然科學基金，21075033，單分子遠場納米顯微成像

代表性論文（*為通訊作者）：

1 Xingbo Shi, Xianxin Meng, Lichun Sun, Jinhua Liu, Jing Zheng, Hongwei Gai*, Ronghua Yang, Edward S. Yeung, Observing photophysical properties of quantum dots in air at the single molecule level: advantages in microarray applications, Lab on a Chip, 2010, DOI: 10.1039/c005258b. (Outside front cover)

2 Xiaobing Bu, Huaping Chen, Hongwei Gai*, Ronghuang Yang, Edward S Yeung, Scattering imaging of single quantum dots with dark-field microscopy, Analytical Chemistry, 2009, 81, 7507-7509.

3 Huaping Chen, Hongwei Gai*, Edward S Yeung, Inhibition of Photobleaching and Blue Shift in Quantum Dots, Chemical Communications, 2009, 1676-1678.

4 Nan Li, Hui Tang, Hongwei Gai,* Xiuling Dong, Qi Wang, Edward S Yeung, Determination of protein surface excess on a liquid/solid interface by single-molecule counting, Analytical and Bioanalytical Chemistry, 2009, 394, 1879-1885.

5 Rui Han, Yewang Zhang, Xiuling Dong, Hongwei Gai*, Edward Yeung, Spectral imaging of single molecules by transmission grating-based epi-fluorescence microscopy, Analytica Chimica Acta, 2008, 619, 209-214.

6 Qun Li, Rui Han, Xianxin Meng, Hongwei Gai*, Edward Yeung, Tracking single quantum dot and its spectrum in free solution with controllable thermal diffusion suppression, Analytical Biochemistry, 2008, 377 , 176–181.

7 Hongwei Gai, Isaac Stayton, Xin Liu, Bingcheng Lin, Yinfa Ma, Visualizing chemical interactions in life sciences with wide-field fluorescence microscopy towards the single-molecule level, Trends in Analytical Chemistry, 2007, 26, 980-992.

8 Hongwei Gai, Gary A. Griess, Borries Demeler, Susan T. Weintraub, Philip Serwer, Routine fluorescence microscopy of single untethered protein molecules confined to a planar zone, Journal of Microscopy, 2007, 226, 256–262.

9 Hongwei Gai, Qi Wang, Yinfa Ma, Bingcheng Lin, Correlations between molecular numbers and molecular masses in an evanescent field and their applications in probing molecular interactions. Angewandte Chemie International Edition, 2005, 44, 5107-5110.

10 Hongwei Gai, Ying Li, Zhan-Hua Silber-Li, Yinfa Ma, Bingcheng Lin, Simultaneously measurements of bulk flow and near wall flow velocity in a microchannel at single molecule level, Lab on a Chip, 2005, 5, 443-449.

11 Hongwei Gai, Linfen Yu, Zhongpeng Dai, Yinfa Ma, Bingcheng Lin., Injection by Hydrostatic Pressure Conjunction with Electrokinetic Force on a Microfluidic Chip, Electrophoresis, 2004, 25,1888-1894.