朱冬青
武漢大學藥學院副教授
2008.9―2011.8布朗大學化學系 Post doctoral research associate
2011.9― 武漢大學藥學院副教授
對具有重要基礎和應用價值的活性天然產物的生物合成機制及其分子改造進行了多年的探索和積累,在國外權威學術期刊,如“Journal of the American Chemical Society”、“Biochemistry”、 “Journal of Bacteriology”發表研究論文多篇。
1. Qiu J, Zhuo Y, Zhu D, Zhou X, Zhang L, Bai L, Deng Z. Overexpression of the ABC transporter AvtAB increases avermectin production inStreptomyces avermitilis. Appl Microbiol Biotechnol. 2011 Oct;92(2):337-45.
2. Zhu D*, Seo MJ*, Ikeda H, Cane DE. Genome mining in streptomyces. Discovery of an unprecedented P450-catalyzed oxidative rearrangement that is the final step in the biosynthesis of pentalenolactone.(*, co-first author) J Am Chem Soc. 2011 Feb 23;133(7):2128-31.
3. Seo MJ*, Zhu D*, Endo S, Ikeda H, Cane DE. Genome mining in Streptomyces. Elucidation of the role of Baeyer-Villiger monooxygenases and non-heme iron-dependent dehydrogenase/oxygenases in the final steps of the biosynthesis of pentalenolactone and neopentalenolactone.(*, co-first author) Biochemistry. 2011 Mar 15;50(10):1739-54.
4. Zhao C, Coughlin JM, Ju J, Zhu D, Wendt-Pienkowski E, Zhou X, Wang Z, Shen B, Deng Z. Oxazolomycin biosynthesis inStreptomyces albusJA3453 featuring an "acyltransferase-less" type I polyketide synthase that incorporates two distinct extender units. J Biol Chem. 2010 Jun 25;285(26):20097-108.
5. He Y, Zhu D, Bai L, Deng Z. Cloning of Antibiotic Biosynthetic Gene clusters from Genomic library by Narrow-down PCR. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY. 2009; 43(1):5-8. Chinese
6. Zhu D, He Y, Bai L, Deng Z. Positive Regulation of Avermectin Biosynthesis by AveR inStreptomyces avermitili. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY. 2008; 42(9):1448-1452. Chinese
7. Zhu D, He X, Zhou X, Deng Z. Expression of themelCoperon in severalStreptomycesstrains is positively regulated by AdpA, an AraC family transcriptional regulator involved in morphological development inStreptomyces coelicolor. J Bacteriol. 2005 May;187(9):3180-7.
8. Zhang X, Zhu D, Wang D, Lin J, Qu Y, Yu S. [Study on xylose fermentation by Neurospora crassa].Wei Sheng Wu Xue Bao. 2003 Aug;43(4):466-72. Chinese.
9. Zhang X, Zhu D, Wang D, Lin J, Qu Y, Yu S. [Production and Some Properties of Xylanase from Neurospora crassa]. JOURNAL OF CELLULOSE SCIENCE AND TECHNOLOGY . 2002; 10(04). Chinese
從微生物中尋找具有重要基礎研究和實際應用價值的活性天然產物,調取參與天然產物生物合成的基因簇,通過體內遺傳學研究和體外生物化學研究,探索活性天然產物的生物合成途徑以及酶催化機制。希望在分子水平上解決一些基礎科學研究的問題,為酶催化產物改造提供理論依據,同時也為天然產物的人工改造,發現和篩選新葯提供更有效的,更有針對性的途徑和方法。
微生物同樣存在複雜而有序的調節網路,對環境、群體、以及細胞內的各種變化做出反應,調節細胞內的主代謝、次生代謝、和形態分化。通過對調節基因的研究,一方面可以完善微生物細胞內的調控網,了解各種代謝途徑是如何互相影響形成有機的整體。另一方面,對調節基因改造是提高抗生素產量的最直接最有效的途徑之一,有非常重要的應用價值。
布朗大學化學系教授, Vernon K. Krieble 化學教授和生化教授David E. Cane