張興華

2004.09-2009.07中國科學院物理研究所 博士 軟物質物理與生物物理

2000.09-2004.07 山東大學物理學院 學士 物理學基地班

2015.11-：研究員，博士生導師 武漢大學生命科學學院，武漢大學高等研究院

2010.03-2015.10：研究科學家 新加坡-MIT聯合科研中心生物系統與微觀力學實驗室

2009.08-2010.03：專利審查員 國家知識產權局

我們主要使用單分子磁鑷技術操縱核酸或蛋白分子，在單分子水平上研究分子結構及分子間相互作用。

1.操縱單個核酸分子可以研究核酸結構的多樣性，還可以研究核酸與蛋白質的相互作用。在該方向，張興華以第一作者在《PNAS》上發表論文2篇、在《JACS》上發表論文1篇。核酸結構的多樣性、RNA病毒解旋酶將是我們未來幾年的研究重點。

2.操縱單個蛋白質分子可以研究蛋白質的摺疊和去摺疊，還可以研究蛋白質與配體之間的相互作用。膠原蛋白與其配體的特異性相互作用將是我們未來幾年的研究重點。

1)Xinghua Zhang, Yuanyuan Qu, Hu Chen, Ioulia Rouzina, Shengli Zhang, Patrick S. Doyle*, and Jie Yan*.(2014). Interconversion between three overstretched DNA structures.J. Am. Chem. Soc.136: 16073-16080.

2)Xinghua Zhang, Hu Chen, Shimin Le, Ioulia Rouzina, Patrick S. Doyle*, and Jie Yan*.(2013).Revealing the competition between peeled ssDNA, melting bubbles and S-DNA during DNA overstretching by single-molecule calorimetry.Proc. Natl. Acad. Sci. U.S.A.110:3865-3870.

3)Xinghua Zhang, Hu Chen, Hongixa Fu, Patrick S. Doyle*, and Jie Yan*.(2012). Two distinct overstretched DNA structures revealed by single-molecule thermodynamics measurements.Proc. Natl. Acad. Sci. U.S.A.109:8103-8108.

4) Shimin Le, Hu ChenXinghua Zhang, Jin Chen, Neelakanteshwar Patil, Kalappa Muniyappad, and Jie Yan*.(2014).Mechanical force antagonizes the inhibitory effects of RecX on RecA filament formation in Mycobacterium tuberculosis.Nucleic. Acids. Res.42: 11992-11999.

5) Hongxia Fu, Hu Chen,Xinghua Zhang, Yuanyuan Qu, John F. Marko and Jie Yan*.(2011) .Transition dynamics and selection of the distinct S-DNA and strand unpeeling modes of double helix overstretching.Nucleic. Acids. Res.39:3473-348 .

6) Ximiao Hou,Xinghua Zhang, Kongji Wei, Chao Ji, Shuoxing Dou, Weichi Wang, Ming Li , Pengye Wang.(2009).Cisplatin induces loop structures and condensation of single DNA molecules.Nucleic. Acids. Res.37: 1400-1410.

7)Xinghua Zhang, Bin Xiao, Ximiao Hou, Chunhua Xu, Pengye Wang and Ming Li*.(2009).Study of cisplatin-induced DNA compaction using single molecule magnetic-tweezers.Acta Physica Sinica. 58: 4301-4306.

8)Xinghua Zhang, Ximiao Hou, Chao Ji, Ming Li and Pengye Wang*.(2009).Spermidine-induced two dimensional DNA condensation on mica surface: a different pathway from the condensation in solution.Chinese Science Bulletin. 54:2425-2433

9) Bo Sun, Kongji Wei, Bo Zhang,Xinghua Zhang, ShuoXing Dou, Ming Li* and Xuguang Xi*.(2008).Impediment of E. coli UvrD by DNA-destabilizing force reveals a strained-inchworm mechanism of DNA unwinding.EMBO J.27:3279-3287.

10) Wenbo Fu, Xiaoling Wang,Xinghua Zhang, Shiyong Ran, Jie Yan and Ming Li.(2006).Compaction dynamics of single DNA molecules under tension.J. Am. Chem. Soc.128, 15040-15041.