何正國

1988—1992 華中農業大學 微生物學 本科

1992 —1995 華中農業大學 微生物學碩士

1998 —2001 中國科學院和丹麥哥本哈根大學 博士

2001 -2004 美國哈佛大學醫學院作博士后研究

2005 至今 華中農業大學生命科學技術學院生物科學系任教授；博士生導師。

2010年當選為華中農業大學生命科學技術學院副院長

2010 獲國家傑出青年科學基金 資助

從事病原微生物的新功能基因和蛋白質組學研究以及創新藥物和疫苗的開發。

從事極端微生物和病原微生物的分子生物學研究，在環境微生物生物技術及微生物的生物化學與分子生物學領域取得了創新成果。

華中農業大學蛋白質組學研究中心，生命科學技術學院生物科學系，農業微生物學國家重點實驗室

①在美國哈佛大學醫學院學習期間，師從美國科學院院士Charles C. Richardson教授開展T7噬菌體病毒DNA複製的研究，發現了病毒的單鏈DNA結合蛋白C-末端小肽對其DNA聚合酶、DNA解旋酶以及引發酶等多個複製關鍵酶的功能有重要調控作用，在國際著名雜誌JBC上發表研究論文2篇，有關研究結果最近被Nature等雜誌引用；

②系統開展了中國西北某地質油藏的極端微生物與石油的相互作用研究，發現了具有重要功能的微生物類群，整合外源優勢微生物研製了能夠提高石油採收率的生物技術，在中國油田首期應用獲得了較大經濟效益並有深遠的應用前景，成果在美國的油氣雜誌作為焦點（Focus）論文發表；

③通過開展分離自雲南的具有我國資源特色的極端嗜酸熱古菌研究，成功從高溫微生物中克隆了硫氧化還原酶基因並首次在大腸桿菌中高效表達了熱穩定性的酶，結果在歐美核心期刊發表研究論文3篇，獲得國家授權發明專利2項，有關研究最近被Science等雜誌引用。

1. Manman Guo, Hui Feng, Jun Zhang, Wenqin Wang, Yi Wang, Yuqing Li, Chunhui Gao, Huanchun Chen, Ying Feng and Zheng-Guo He（通訊作者）.　Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Reseearch. 2009. 19: 1301-1308 . IF= 11.342

2. Lu Zhang, Lei Zhang, Yi Liu, Shifan Yang, Chunhui Gao, Hongchao Gong, Ying Feng, and Zheng-Guo He (通訊作者).Archaeal eukaryote-like Orc1/Cdc6 initiators physically interact with DNA polymerase B1 and regulate its functions. PNAS, 2009 vol. 106 no. 19 7792-7797 .IF= 9.432

3. Feng Huang and Zheng-Guo He* (correspond author).Characterization of an interplay between a Mycobacterium tuberculosis MazF homolog, Rv1495 and its sole DNA topoisomerase I . Nucl. Acids Res. (2010) doi: 10.1093/nar/gkq737. IF = 7.479

4.Bin Wang,Shifan Yang,Lei Zhang,and Zheng-Guo He(correspond author).　Archaeal Eukaryote-Like Serine/Threonine Protein Kinase Interacts with and Phosphorylates a Forkhead-Associated-Domain-Containing Protein .Journal of Bacteriology, 2010, Vol. 192,p. 1956-1964. IF= 3.94

5. Lei Zhang and Zheng-Guo He* (correspond author) , Radiation-sensitive gene A (RadA) targets DisA, DNA integrity scanning protein A, to negatively affect its cyclic-di-AMP synthesis activity in Mycobacterium smegmatis . J. Biol. Chem. 2013

6. Lei Zhang, Weihui Li, and Zheng-Guo He* (correspond author) . DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis . J. Biol. Chem. 2013,288: 3085-3096.

7 . Muding Rao, Huicong Liu, Min Yang, Chunchao Zhao, and Zheng-Guo He* (correspond author). A Copper-responsive Global Repressor Regulates Expression of Diverse Membrane-associated Transporters and Bacterial Drug Resistance in Mycobacteria. J. Biol. Chem. 2012, 287: 39721-39731.

8. Yi Wang, Yuanxia Huang, Chaolun Xue, Yang He, and Zheng-Guo He* (correspond author). ClpR Protein-like Regulator Specifically Recognizes RecA Protein-independent Promoter Motif and Broadly Regulates Expression of DNA Damage-inducible Genes in Mycobacteria. J. Biol. Chem. 2011 286: 31159-31167.

9. Weihui Li and Zheng-Guo He* (correspond author). LtmA, a novel cyclic di-GMP-responsive activator, broadly regulates the expression of lipid transport and metabolism genes in Mycobacterium smegmatis. Nucl. Acids Res. (2012) 40 (22): 11292-11307

10. Min Yang, Chunhui Gao, Tao Cui, Jingning An, and Zheng-Guo He* (correspond author) . A TetR-like regulator broadly affects the expressions of diverse genes in Mycobacterium smegmatis. Nucl. Acids Res. (2012) 40 (3): 1009-1020

11. Yuqing Li, Jumei Zeng, Jianfang Shi, Mingchao Wang, Muding Rao, Chaolun Xue, Yanli Du and Zheng-Guo He* (correspond author) .A Proteome-Scale Identification of Novel Antigenic Proteins in Mycobacterium tuberculosistoward Diagnostic and Vaccine Development . J. Proteome Res., 2010

12. Yi Wang, Tao Cui, Cong Zhang, Min Yang, Yuanxia Huang, Weihui Li, Lei Zhang, Chunhui Gao, Yang He, Yuqing Li, Feng Huang, Jumei Zeng, Cheng Huang, Qiong Yang, Yuxi Tian, Chunchao Zhao, Huanchun Chen, Hua Zhang, and Zheng-Guo He* (correspond author). Global Protein−Protein Interaction Network in the Human Pathogen Mycobacterium tuberculosisH37Rv. J. Proteome Res. , 2010

13. Jumei Zeng, Tao Cui, and Zheng-Guo He* (correspond author).A Genome-Wide Regulator–DNA Interaction Network in the Human Pathogen Mycobacterium tuberculosisH37Rv. J. Proteome Res. , 2012

14.Zheng-Guo He, Charles Richardson (2004) Effect of single-stranded DNA-binding proteins on the helicase and primase activities of bacteriophage T7 gene 4 protein. Journal of Biological Chemistry. 279(21):22190-22197. IF:5.854

15.Zheng-Guo He, Lisa Rezende, S Willocox, Jack Griffith, Charles Richardson (2003) The carboxyl-terminal domain of bacteriophage T7 single-stranded DNA-binding protein modulates DNA binding and interaction with T7 DNA polymerase. Journal of Biological Chemistry. 278(32): 29538-29545. IF:5.854

16. Zheng-Guo He, Huifang Zhong, Yaqin Li. (2004) Acidianus tengchongenesis. sp.nov., a new species of acidothermophilic archaeon from hot spring. Current Microbiology. 48(2): 159-163. IF:1.059 8 . Zhengguo He, Li Y, Zhou P, Liu S-J. (2000). Cloning and heterologeous expression of sulfur oxygenase/reductase gene of a thermoacidophilic archae Acidianus sp. S5 in Escherichia coli. FEMS Microbiological Letter 193(2):217-221. IF:2.507

近年來共發表SCI論文三十多篇，EI收錄論文3篇，獲得國家授權發明專利2項。以主要身份參與了美國國家衛生研究院（NIH）項目、歐共體項目、國家自然科學基金重點項目，科技部重大專項，以及國際合作項目等課題的研究。目前主持國家自然科學基金，湖北省青年傑出人才基金，教育部留學回國基金，華中農業大學高層次引進人才啟動基金等項目的研究。

現主持6項國家及省部級科研課題，2006年入選教育部新世紀優秀人才資助計劃。2010年 獲國家傑出青年科學基金 資助。