王欣

1981.9-1985.6：山東大學生物系獲學士學位；1987.9-1990.6：北京醫科大學獲醫學遺傳學碩士學位；1992.9-1998.6：美國State University of New York at Buffalo獲分子藥理學Ph.D.；1998-2006：先後在美國Oregon Health & Science University和University of Minnesota stem Cell Institute and Department of Laboratory Medicine and Pathology做博士后及擔任助理教授(tenure track)。

肝臟幹細胞

細胞治療是指通過移植相應組織的細胞以修複發生了疾病、衰老或損傷的組織器官，從而更新其生理功能的一種新的治療方法。幹細胞尤其是胚胎幹細胞具有可以在體外大量增殖的特性，同時在一定的誘導條件下又可以分化為多種類型的細胞，因此非常有希望成為細胞治療中的細胞來源。

本人基於以上的出發點，對幹細胞向功能性肝臟細胞的分化進行了探索研究。

(1)對血液幹細胞逆分化為肝臟實質細胞的功能鑒定。

(2)發現胰腺幹細胞具有多能性，能夠分化為肝實質細胞。

(3)肝臟原位卵圓形幹細胞的分離純化和移植鑒定。

(4)血液幹細胞與肝實質細胞在體內融合，能夠使肝實質細胞重編程。

今後，我們將對胚胎幹細胞向肝臟細胞的誘導分化做進一步的研究。

1. Wangensteen K, Wilber AC, Keng VW, Chen Y, Matise I, Wangensteen L, Steer CL, Zhuo L, Frandsen JL, Bell J, Chen Z, McIvor RS and Wang X*, Ekker SC* (2007) “A facile method for somatic, lifelong manipulation of multiple genes in the mouse liver” Hepatology (In press)

2. Jin CX, L WL, Xu F, Geng ZH, He ZY, Su J, Ding XY, Wang X* and Hu YP* (2007) “Conversion of Immortal Liver Progenitor Cells into Pancreatic Endocrine Progenitor Cells by Persistent Expression of Pdx-1” Journal of Cellular Biochemistry (in press)

3. Li WL, You P, Wei Q, Li Y, Fu XP, Ding XY, Wang X*, Hu YP*. (2007) “Hepatic Differentiation and Transcriptional Profiling of the Mouse Liver Epithelial Progenitor Cells (LEPCs) under the Induction of Sodium Butyrate” Frontiers in Bioscience. 12:1691-1698.

4. Wilber AC, Wangensteen K, Chen Y, Zhuo L, Frandsen JL, Bell J, Chen Z, Ekker SC, McIvor RS and Wang X* (2007) “Tracking Transposon-Mediated Correction of Hereditary Tyrosinemia Type I as Determined by In vivo Co-expression of a Bioluminescent Reporter when Messenger RNA is Used as a Source of Transposase” Molecular Therapy 15:1280-1287.

5. Balciunas D, Wangensteen KJ, Wilber A, Bell J, Geurts A, Sivasubbu S, Wang X, Hackett PB, Largaespada DA, McIvor RS, Ekker SC. (2006) “Free in PMC Harnessing a high cargo-capacity transposon for genetic applications in vertebrates” PLoS Genet. 2 (11): 1715-1724.

6. Andrew C. Wilber, Joel L. Frandsen, Kirk Wangensteen, Stephen C. Ekker, Xin Wang, and R. Scott McIvor (2005) “Dynamic Gene Expression Following Systemic Delivery of Plasmid DNA as Determined by In vivo Bioluminescence Imaging” Human Gene Therapy. 16 (11): 1325-32.

7. Wang, X., Foster, M., Al-Dhalimy, M., Lagasse, E., Finegold, M., Grompe, M (2003) “The origin and liver repopulating capacity of murine oval cells”PNAS. 100 S1:11881-8.

8. Wang, X, Willenbring, H, Akkari, Y, Torimaru, Y, Foster, M, Al-Dhalimy, M, Lagasse, E, Finegold, F, Olson, S, and Markus Grompe. (2003)“Cell fusion is the principal source of bone-marrow-derived hepatocytes” Nature. 422: 897-901.

9. Wang, X., Montini, E., M. Al-Dhalimy, M. Lagasse, E., Finegold and M. Grompe (2002) “Kinetics of liver repopulation by bone marrow transplantation” The American Journal of Pathology. 161 (2): 565-574.

10. Wang, X., Al-Dhalimy, M., Lagasse, E., Finegold, M., Grompe, M. (2001) “Liver repopulation and correction of metabolic liver disease by transplanted adult mouse pancreatic cells” The American Journal of Pathology. 158 (2): 571- 579.

11. Lagasse, E., Connors, H., Al-Dhalimy, M., Reitsma, M., Dohse, M., Osborne, L., Wang, X., Finegold, M., Weissman, I.L. and Grompe, M. (2000) “Purified Hematopoietic Stem Cells Can Differentiate into Hepatocytes In Vivo” Nature Medicine. 6 (11): 1229-1234.

Invited book Chapters/Review articles:

Hu Y, Chen JZ, Ding XY, Wang X* (2007) Chapter of Liver stem cells in regenerative medicine in “Stem Cells and Regenerative Medicine”, World Scientific Publishing Co. (In press)