王宏偉

1992-1996 清華大學生物科學與技術系，學士

1996-2001 清華大學生物科學與技術系，博士

2001-2006 美國勞倫斯伯克利國家實驗室生命科學部，博士后

2006-2008 美國勞倫斯伯克利國家實驗室生命科學部，研究科學家

2009-2011 美國耶魯大學分子生物物理與生物化學系，Tenure-Track助理教授

2011.07-現在 北大-清華生命科學聯合中心 研究員（Principle Investigator, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China）

2014.01-2018.12 美國耶魯大學分子生物物理與生物化學系兼職教授（Adjunct Associate Professor, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA.）

2010.12 清華大學生命科學學院，教授、博導

2016年4月 清華大學生命科學學院院長。

利用冷凍電子顯微學研究生物大分子複合體的結構與分子機理。

主要研究方向如下：

1. 更高效、更高解析度的冷凍電鏡方法學開發；

2. 細胞骨架和生物膜系統的協調機制；

3. 核酸質量控制的分子機制和調控。

長期致力於使用冷凍電鏡技術研究生物大分子的結構以及其組裝機制，研究領域集中在細胞骨架動態機理、RNA降解、RNA干擾以及冷凍電鏡方法學。目前已獲得微管和Exosome等的高分辨電鏡結構。此外，還與其他課題組合作，解析了一批具有重要生物學意義的生物大分子的結構。

論文

1. Rao Q, Liu M, Tian Y, Wu Z, Hao Y, Song L, Qin Z, Ding C, Wang HW*, Wang J*, Xu Y*. (2018) Cryo-EM structure of human ATR-ATRIP complex. Cell Research, 28(2):143-156

2. Liu Z, Wang J, Cheng H, Ke X, Sun L, Zhang Q, Wang HW*. (2018) Cryo-EM Structure of Human Dicer and Its Complexes with a Pre-miRNA Substrate. Cell, 173(5):1191-1203

3. Mei K, Li Y, Wang S, Shao G, Wang J, Ding Y, Luo G, Yue P, Liu JJ, Wang X, Dong MQ, Wang HW*, Guo W*. (2018) Cryo-EM structure of the exocyst complex. Nat. Struct. Mol. Biol., 25(2):139-146

4. Fan X, Zhao L, Liu C, Zhang JC, Fan K, Yan X, Peng HL, Lei J*, Wang HW*. (2017) Near-Atomic Resolution Structure Determination in Over-Focus with Volta Phase Plate by Cs-Corrected Cryo-EM. Structure, 25:1623-1630.

5. Ma M, Liu JJ, Li Y, Huang Y, Ta N, Chen Y, Fu H, Ye MD, Ding Y, Huang W, Wang J, Dong MQ, Yu L*, Wang HW*. (2017) Cryo-EM structure and biochemical analysis reveal the basis of the functional difference between human PI3KC3-C1 and -C2. Cell Research, 27(8):989-1001

6. Zheng JX, Li Y, Ding YH, Liu JJ, Zhang MJ, Dong MQ, Wang HW*, Yu L*. (2017) Architecture of the ATG2B-WDR45 complex and an aromatic Y/HF motif crucial for complex formation. Autophagy, 13(11):1870-1883

7. Xu J, Zhao L, Xu Y, Zhao W, Sung P*, and Wang HW*. (2017) Cryo-EM structures of human RAD51 recombinase filaments during catalysis of DNA-strand exchange. Nat. Struct. Mol. Biol., 24:40-46

8. Wang HW*, and Wang JW. (2017) How cryo-electron microscopy and X-ray crystallography complement each other. Protein Sci., 26:32-39 (Review)

9. Wang HW*, Lei J*, and Shi Y*. (2017) Biological cryo-electron microscopy in China. Protein Sci., 26:16-31 (Review)

10. Yang H, Wang J, Liu M, Chen X, Huang M, Tan D, Dong MQ, Wong CC, Wang J*, Xu Y*, and Wang HW*. (2016) 4.4 A Resolution Cryo-EM structure of human mTOR Complex 1. Protein Cell, 7:878-887

11. Agrawal RK*, Wang HW*, and Belfort M*. (2016) Forks in the tracks: Group II introns, spliceosomes, telomeres and beyond. RNA Biol., 13:1218-1222

12. Liu JJ, Niu CY, Wu Y, Tan D, Wang Y, Ye MD, Liu Y, Zhao W, Zhou K, Liu QS, Dai J, Yang X, Dong MQ, Huang N, and Wang HW*. (2016) CryoEM structure of yeast cytoplasmic exosome complex. Cell Research, 26:822-837

13. Qu G, Kaushal PS, Wang J, Shigematsu H, Piazza CL, Agrawal RK*, Belfort M*, and Wang HW*. (2016) Structure of a group II intron in complex with its reverse transcriptase. Nat. Struct. Mol. Biol., 23:549-557

14. Qin S, Yin H, Yang C, Dou Y, Liu Z, Zhang P, Yu H, Huang Y, Feng J, Hao J, Hao J, Deng L, Yan X, Dong X, Zhao Z, Jiang T, Wang HW, Luo SJ, and Xie C*. (2016) A magnetic protein biocompass. Nat Mater., 15:217-226

15. Jia N, Liu N, Cheng W, Jiang YL, Sun H, Chen LL, Peng J, Zhang Y, Ding YH, Zhang ZH, Wang X, Cai G, Wang J, Dong MQ, Zhang Z, Wu H, Wang HW*, Chen Y*, and Zhou CZ*. (2016) Structural basis for receptor recognition and pore formation of a zebrafish aerolysin-like protein. EMBO Reports, 17:235-248

16. Wang J, Wang W, Song W, Han Z, Zhang H, Chai J*, Wang HW*, and Wang J*. (2015) An improved method for phasing crystal structures with low non-crystallographic symmetry using cryo-electron microscopy data. Protein Cell, 6:919-923 (Letter)

17. Wang HW*. (2015) Opening new doors for understanding eukaryotic RNA splicing.Sci China Life Sci., 58:1171-1172 (Editorial Material)

18. Hu Z, Zhou Q, Zhang C, Fan S, Cheng W, Zhao Y, Shao F, Wang HW, Sui SF*, and Chai J*. (2015) Structural and biochemical basis for induced self-propagation of NLRC4. Science, 350:399-404

19. Tomko RJ Jr*, Taylor DW, Chen ZA, Wang HW, Rappsilber J, and Hochstrasser M*. (2015) A single α helix drives extensive remodeling of the proteasome lid and completion of regulatory particle assembly. Cell, 163:432-444

20. Song W, Wang J, Han Z, Zhang Y, Zhang H, Wang W, Chang J, Xia B, Fan S, Zhang D, Wang J*, Wang HW*, and Chai J*. (2015) Structural basis for specific recognition of single-stranded RNA by Toll-like receptor 13. Nat. Struct. Mol. Biol., 22, 782-787.

21. Zhou Q, Huang X, Sun S, Li X, Wang HW*, and Sui SF*. (2015) Cryo-EM structure of SNAP-SNARE assembly in 20S particle. Cell Research, 25:551-560.

22. Chang S, Sun D, Liang H, Wang J, Li J, Guo L, Wang X, Guan C, Boruah BM, Yuan L, Feng F, Yang M, Wang L, Wang Y, Wojdyla J, Li L, Wang J, Wang M, Cheng G, Wang HW*, and Liu Y*. (2015) Cryo-EM Structure of Influenza Virus RNA Polymerase Complex at 4.3 A Resolution. Mol. Cell, 57:925-935.

23. Liu JJ, Bratkowski MA, Liu X, Niu CY, Ke A*, and Wang HW*. (2014) Visualization of distinct substrate-recruitment pathways in the yeast exosome by EM. Nat. Struct. Mol. Biol., 21:95-102.

24. Li Y, Hsin J, Zhao L, Cheng Y, Shang W, Huang KC, Wang HW, and Ye S*. (2013) FtsZ protofilaments use a hinge-opening mechanism for constrictive force generation. Science, 341:392-295.

25. Taylor DW, Ma E, Shigematsu H, Cianfrocco MA, Noland CL, Nagayama K, Nogales E, Doudna JA*, and Wang HW*. (2013) Substrate-specific structural rearrangements of human Dicer. Nat. Struct. Mol. Biol., 20:662-670.

26. Chen X, Taylor DW, Fowler CC, Galan JE, Wang HW, and Wolin SL*. (2013) An RNA degradation machine sculpted by Ro autoantigen and noncoding RNA. Cell, 153:166-177.

27. Shen QT, Hsiue PP, Sindelar CV, Welch MD, Campellone KG*, and Wang HW*. (2012) Structural insight into WHAMM-mediated cytoskeletal coordination during membrane remodeling. J. Cell Biol., 199:111-124.

28. Zhou K, Kanai R, Lee P, Wang HW*, and Modis Y*. (2012) Toll-like receptor 5 forms asymmetric dimers in the absence of flagellin. J. Struct. Biol., 177:402-409.

29. Liu XQ, and Wang HW*. (2011) Single particle electron microscopy reconstruction of the exosome complex using the random conical tilt method. J. Vis. Exp., doi: 10.3791/2574.

30. Wu X, Shen QT, Stokes N, Lu C, Zheng Q, Polak L, Wang HW, and Fuchs E*. (2011) Skin stem cells orchestrate directional migration by regulating microtubule-ACF7 connections through GSK3β. Cell, 144:341-352.

31. Wang HW*, Noland C, Siridechadilok B, Taylor DW, Ma E, Felderer K, Doudna JA*, and Nogales E*. (2009) Structural insights into RNA processing by the human RISC-loading complex. Nat. Struct. Mol. Biol., 16:1148-1153.

32. Wang HW, Long S, Ciferri C, Westermann S, Drubin DG, Barnes G, and Nogales E*. (2008) Architecture and flexibility of the yeast Ndc80 kinetochore complex. J. Mol. Biol., 383:894-903.

33. Wang HW*, Wang J, Ding F, Callahan K, Bratkowski MA, Bulter JS, Nogales E, and Ke A*. (2007) Architecture of the yeast Rrp44-exosome complex suggests routes of RNA recruitment for 3’-end processing. Proc. Natl. Acad. Sci. USA, 104:16844-16849.

34. Wang HW, Ramey VH, Westermann S, Leschziner A, Welburn JPI, Nakajima Y, Drubin DG, Barnes G, and Nogales E*. (2007) Architecture of the Dam1 kinetochore ring complex and implications for microtubule-driven assembly and force-coupling mechanisms. Nat. Struct. Mol. Biol., 14:721-726.

35. Westermann W, Wang HW, Avila-Sakar A, Drubin DG, Nogales E, and Barnes G*. (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature, 440:565-569.

36. Wang HW, and Nogales E*. (2005) The nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly. Nature, 435:911-915.