李大偉

1985.7--1988.8，河南農業大學植保系助教。

1995.12--1997.11，中國科學院遺傳所博士后。

1997.12--2002.12，中國農業大學農業生物技術國家重點實驗室副教授。

1999.11--2002.8，美國University of Colorado Health Sciences Center生化與分子遺傳學系博士后。

2002.12--今，中國農業大學生物學院微生物學與免疫學系及農業生物技術國家重點實驗室教授、博士生導師。

2007.3--2007.9，美國Oregon State University的James Carrington教授實驗室高級研究學者。

2006.5-今，中國農業生物技術學會常務理事。

2005.4-今，中國農業生物技術學會微生物技術分會副會長。

2004.4-2009.12，Chinese Journal of Agricultural Biotechnology(英國CABI集團出版)特邀編輯。

研究方向

主要從事分子植物病毒學的基礎理論及應用基礎研究，研究內容包括：

（1）大麥條紋花葉病毒（BSMV）對不同寄主植物致病性差異的分子機制以及抗BSMV新基因的功能分析；建立並完善BSMV誘導的基因沉默（BSMV-based VIGS）載體系統，為小麥、大麥、玉米、高粱和穀子等禾本科作物的基因功能分析提供高效、便捷的工具。

（2）以甜菜黑色焦枯病毒（BBSV）和煙草壞死病毒A（TNV-A）等病毒為研究對象，分析病毒和寄主植物的相互作用以及互作過程中的一些重要蛋白質的晶體結構解析。

（3）抗病毒轉基因作物新品種(系)的培育。

論文

Gerda Szakonyi, Joel M Guthridge,Dawei Li, Young Kendra, Michael V Holers, Xiaojiang S Chen, 2001, Structure of complement receptor 2 in complex with its C3d ligand,Science, 292: 1725-1728.

Dawei Li, Rui Zhao, Wayne Lilyestrom, Dahai Gai, Rongguang Zhang, DeCaprio James, Ellen Fanning, Gerda Szakonyi, Xiaojiang S Chen, 2003, Structure of the replicative helicase of the oncoprotein SV40 large tumour antigen,Nature, 423: 512-518.

Dahai Gai,Dawei Li, Carla V Finkielstein, Robert D Ott, Poonam Taneja, Ellen Fanning, Xiaojiang S Chen, 2004, Insights into the oligomeric states, conformational changes and helicase activities of SV40 large tumor antigen,Journal of Biological Chemistry, 279: 38952-38959.

Dahai Gai, Rui Zhao,Dawei Li, Carla V Finkielstein, Xiaojiang S Chen, 2004, Mechanisms of conformational change for a replicative hexameric helicase of SV40 large T antigen,Cell, 119: 47-60.

Lihua Guo, Yunhe Cao,Dawei Li,Shengniao Niu, Zhunan Cai, Chenggui Han, Yafeng Zhai, Jialin Yu, 2005, Analysis of nucleotide sequences and multimeric forms of a novel satellite RNA associated with Beet black scorch virus,Journal of Virology, 79: 3664-3674 .

Yunhe Cao, Xuefeng Yuan, Xiaoxing Wang, Lihua Guo, Zhunan Cai, Chenggui Han,Dawei Li*, Jialin Yu, 2006, Effect on viral pathogenicity of Beet black scorch virus coat protein,Progress in Biochemistry and Biophysics, 33: 127-134.

Zhaohui Wang, Shuoguo Fang, Zhiyan Zhang, Chenggui Han,Dawei Li*, Jialin Yu, 2006, Development of an ID-ELISA for the detection of Rice black-streaked dwarf virus in plants,Journal of Virological Methods, 134: 61-65.

Xuefeng Yuan, Yunhe Cao, Dehui Xi, Lihua Guo, Chenggui Han,Dawei Li*, Yafeng Zhai, Jialin Yu, 2006, Analysis of the subgenomic RNAs and the small ORFs of Beet black scorch virus by in vitro transcripts,Journal of General Virology, 78: 3077-3086.

Dehui Xi, Jiang Li, Chenggui Han,Dawei Li*, Jialin Yu, Xueping Zhou, 2008, Complete nucleotide sequence of a new strain of Tobacco necrosis virus A infecting soybean in China and infectivity of its full-length cDNA clone,Virus Genes, 36: 259-266.

Montgomery T A, Howell M, Cuperus J T,Dawei Li, Alexander A, Chapman E J, Fahlgren N, Allen E, and James C Carrington, 2008, Specificity of ARGONAUTE7-miR390 Interaction and Dual Functionality of ARGONAUTE7-miR390 Complexes inTAS3 Trans-Acting siRNA Formation,Cell, 133: 128-141.

Yongliang Zhang, Jiang Li, Heng Pu, Jin Jin, Xiaofeng Zhang, Mingkuan Chen, Bin Wang, Chenggui Han, Jialin Yu,Dawei Li*,2010, Development of Tobacco necrosis virus A as a vector for efficient and stable expression of FMDV VP1 peptides,Plant Biotechnology Journal, 8(4): 506-523.

Bo Zhou, Yongliang Zhang, Xianbing Wang, Jiangli Dong, Bin Wang, Chenggui Han, Jialin Yu,Dawei Li*, 2010, Oral administration of plant-based rotavirus VP6 induces antigen-specific IgAs, IgGs and passive protection in mice,Vaccine, 28(37): 6021-6027.

Yanhong Han, Haiying Xiang, Qian Wang, Yuanyuan Li, Wenqi Wu, Chenggui Han,Dawei Li, Jialin Yu, 2010, Ring structure amino acids affect the suppressor activity ofMelon aphid-borne yellows virusP0 protein.Virology, 406(1): 21-27.

Yanjing Zhang, Xiaofeng Zhang, Shaofang Niu, Chenggui Han, Jialin Yu,Dawei Li*, 2011, Nuclear localization ofBeet black scorch viruscapsid protein and its interaction with importin α,Virus Research, 155(1): 307-315.

Cheng Yuan, Cui Li, Lijie Yan, Andrew O Jackson, Zhiyong Liu, Chenggui Han, Jialin Yu,Dawei Li, 2011, A high throughputBarley stripe mosaic virusvector for virus induced gene silencing in monocots and dicots,PLoS ONE, 6: e26468.

Yu Cui, Mi Yeon Lee, Naxin Huo, Jennifer Bragg, Lijie Yan, Cheng Yuan, Cui Li, Sara J. Holditch, Jingzhong Xie, Ming-Cheng Luo,Dawei Li, Jialin Yu, Joel Martin, Wendy Schackwitz, Yong-Qiang Gu, John P. Vogel, Andrew O. Jackson, Zhiyong Liu1, and David F. Garvin, 2012, Fine mapping of theBarley stripe mosaic virusresistance geneBsr1in the model grassBrachypodium distachyon,PLoS ONE, 7(6): e38333.

Xiuchun Zhang, Xiaofeng Zhang, Jasleen Singh,Dawei Li, and Feng Qu, 2012, Temperature-dependent survival ofTurnip crinkle virus-infected Arabidopsis plants relies on an RNA silencing-based defense that requires DCL2, AGO2, and HEN1,Journal of Virology, 86: 6847-6854.

X徠iaohui Wang, Yanjing Zhang, Jin Xu, Lindan Shi, Huiyan Fan,Daiwei Li, Chenggui Han, Jialin Yu, 2012, The R-rich motif of Beet black scorch virus P7a movement protein is important for the nuclear localization, nucleolar targeting and viral infectivity,Virus Research, 167(2): 207-218.

Deshui Liu, Lindan Shi, Chenggui Han, Jialin Yu, Dawei Li, Yongliang Zhang 2012, Validation of reference genes for gene expression studies in virus-infectedNicotiana benthamianausing quantitative Real-Time PCR.PLoS ONE, 7: e46451.

Jin Xu, Xianbing Wang, Lindan Shi, Yuan Zhou,Dawei Li, Chenggui Han, Ziding Zhang, Jialin Yu, 2012, Two distinct sites are essential for virulent infection and support of variant satellite RNA replication in spontaneousBeet black scorch virusvariants.Journal of General Virology, 93(12): 2718-2728.

Mi Yeon Lee, Lijie Yan, Florien A. Gorter, Brian Y.T. Kim, Yu Cui, Yue Hu, Cheng Yuan, Jessica Grindheim, Uma Ganesan, Zhiyong Liu, Chenggui Han, Jialin Yu,Dawei Li*and Andrew O. Jackson*, 2012,Brachypodium distachyonBd3-1 resistance is elicited by theBarley stripe mosaic virusTriple Gene Block 1 movement protein.Journal of General Virology, 93(12): 2729-2739. (Cover story)

Thi-Sau Dinh, Cuiji Zhou, Xiuling Cao, Chenggui Han, Jialin Yu,Dawei Li, Yongliang Zhang,2012. The complete genome sequence ofSouthern rice black-streaked dwarf virusisolated from Vietnam.The Plant Pathology Journal, 28(4):428-432.

Xiuling Cao, Yingui Lu, Dianping Di, Zhiyan Zhang, He Liu, Lanzhi Tian, Aihong Zhang,Yanjing Zhang, Lindan Shi, Bihong Guo, Jin Xu, Xifei Duan, Xianbing Wang, Chenggui Han, Hongqin Miao*, Jialin Yu*,Dawei Li*,2013, Enhanced virus resistance in transgenic maize expressing a dsRNA-specific endoribonuclease gene fromE. coli.PLoS ONE, 8(4): e60829.

Deshui Liu, Lindan Shi, Chenggui Han, Jialin Yu, Dawei Li, Yongliang Zhang 2012, Validation of reference genes for gene expression studies in virus-infectedNicotiana benthamianausing quantitative Real-Time PCR.PLoS ONE, 7: e46451.

專利情況

授權專利：“甜菜黑色焦枯病毒作為外源基因的表達載體”（ZL02155378.5）