沈亞歐

玉米重要性狀基因功能、玉米基因工程

1. “玉米抗紋枯病相關miRNA的克隆及功能分析”，國家自然科學基金青年基金，2010.01-2012.12，負責人；

2.“調控玉米胚性愈傷組織發生的miRNA功能鑒定”，四川省教育廳重點項目，2011.09-2013.09，負責人；

3. “玉米幼胚胚性愈傷組織誘導過程中重要miRNA及靶轉錄因子的功能研究”，國家自然科學基金面上項目，2013.01-2016.12，負責人；

4. 基於IBM群體的玉米產量性狀全基因組遺傳解析，國家973計劃項目子課題，2014.01-2018.12，負責人；

5.“玉米耐密、耐陰濕、大粒等相關基因的遺傳轉化和材料篩選”，國家重大轉基因專項子課題，2013.01-2017.12，負責人；

6.“玉米幼胚胚性愈傷組織誘導能力全基因組遺傳解析”國家自然科學基金面上項目，2015.01-2018.12，負責人；

7. 玉米愈傷組織再生能力全基因組遺傳解析，四川省傑出青年基金，2016.01-2018.12，負責人；

8. 玉米胚乳蛋白質組平衡和優質蛋白玉米胚乳修飾機理研究，國家自然科學基金重大研究計劃子項目，2014,01-2016,12，負責人

近三年科研經費300萬元，年均100萬元。

1. 沈亞歐，潘光堂，江舟，楊珊，林海建，彭煥偉，張志明，羅旭，馬浪浪，李婉蓉，張兵.“以單倍體玉米莖尖為受體的轉基因育種方法”發明專利，專利號：ZL201210448146.3

2. 張志明，沈亞歐，暢乃迪，潘光堂，林海健，趙茂俊，馬永毅，丁海萍.“農桿菌介導的高效玉米遺傳轉化方法”，發明專利，專利號：ZL200810147825.0

3. 沈亞歐，彭煥偉，楊珊，繆林，潘光堂，張志明，林海建.“一種數顯示溫塗布棒”，實用新型專利，專利號：ZL201220505737.5

4. 沈亞歐, 楊珊，林海建，劉紅軍，潘光堂，繆林,“一種新型的轉基因專用莖尖分生組織侵染皿”，實用新型專利，專利號：ZL201320295177.X

5. 沈亞歐，劉敏，吳光秀，周倫理，潘光堂，林海建. “多用途植物組織培養刀鑷”，實用新型專利，專利號：ZL201420134162.X

6. 沈亞歐，葛飛，吳花拉，彭煥偉，趙雄偉.“定量微量移液器吸頭”，實用新型專利，專利號：ZL201420047742.5

7. 沈亞歐, 劉敏, 張曉玲, 彭煥偉, 高世斌, 林海建. “多用途植物幼胚挑胚組件”, 實用新型專利，專利號：ZL201520224947.0

8. 沈亞歐, 劉敏, 韓善冬, 彭煥偉, 林海建. “愈傷組織洗菌瓶”，實用新型專利，專利號：ZL201420711144.3

9. 沈亞歐 劉敏 李婉蓉 楊勇 彭煥偉 林海建.“水槽式光照培養架”, 實用新型專利，專利號：ZL 201420296109.X

10. 沈亞歐，張嚴玲，吳花拉，彭煥偉，葛飛. “幼苗水培箱及具有該水培箱的生化培養箱”,實用新型專利，專利號：ZL201520425766.4

1. Fei Ge, Hongmei Hu, Xing Huang, Yanling Zhang, Yanli Wang, Zhaoling Li, Chaoying Zou, Huanwei Peng, Lujiang Li, Shibin Gao, Guangtang Pan, Yaou Shen*. Metabolomic and proteomic analysis of maize embryonic callus induced from immature embryo. Scientific Reports (IF=5.28), 2017, 7: 1004

2. Hongjun Liu, Langlang Ma, Xuerong Yang, Lin Zhang, Xing Zeng, Shupeng Xie, Huanwei Peng, Shibin Gao, Haijian Lin, Guangtang Pan, Yongrui Wu, Yaou Shen*. Integrative analysis of DNAs during maize embryo dedifferentiation. BMC Plant Biology, 2017, 17:105

3. Yanling Zhang, Fei Ge, Fengxia Hou, Wenting Sun, Qi Zheng, Xiaoxiang Zhang, Langlang Ma, Jun Fu, Xiujing He, Huanwei Peng, Guangtang Pan, Yaou Shen*. Transcription factors responding to Pb stess in maize. Genes, 2017, 8, 231

4. Fei Ge, Xing Huang, Hongmei Hu, Yanling Zhang, Yanli Wang, Zhaoling Li, Chaoying Zou, Huanwei Peng, Lujiang Li, Shibin Gao, Guangtang Pan, Yaou Shen*. Endogenous small interfering RNAs associated with maize embryonic callus formation. PLoS One, 2017, e0180567

5. Fei Ge, Xu Luo, Xing Huang, Yanling Zhang, Xiujing He, Min Liu, Haijian Lin, Huanwei Peng, Lujiang Li, Zhiming Zhang, Guangtang Pan, Yaou Shen*. Genome-wide analysis oftranscription factors involved in maize embryonic callus formation. Physiologia Plantarum, 2016, doi: 10.1111/ppl.12470

6. Yaou Shen*, Guangtang Pan, Thomas Lubberstedt*. Haploid strategies for functional validation of plant genes. Trends in Biotechnology (IF=11.96), 2015, 33(10):611-620.

7. Cheng Qin, Changshui Yu, Yaou Shen（Co-first author）, Xiaodong Fang, Lang Chen, Jiumeng Min, Ye Yin, Jiping Yu, Kailin Hu, and Zhiming Zhang* et al. Whole-genome sequencing of cultivated and wild peppers provides insights into Capsicum domestication and specialization. PNAS (IF=9.74),2014,111(14):5135-5140

8. Yaou Shen, Zhou Jiang, Xiadong Yao, Zhiming Zhang, Haijian Lin, Maojun Zhao, Hailan Liu, Huanwei Peng, Shujun Li, Guangtang Pan*. Genome expression profile analysis of the immature maize embryo during dedifferentiation, Plos one (IF=4.41), 2012, DOI:10.1371/journal.pone.0032237

9. Yaou Shen, Zhiming Zhang, Haijian Lin, Hailan Liu, Jie Chen, Hua Peng, Moju Cao, Tingzhao Rong, Guangtang Pan*. Cytoplasmic male sterility-regulated novel microRNAs from maize. Funct Integr Genomics (IF=3.82), 2011, 11(1):179-191

10. Yaou Shen, Yongzhong Zhang, Jie Chen, Haijian Lin, Maojun Zhao, Huanwei Peng, Li Liu, Guangsheng Yuan, Zhiming Zhang, Suzhi Zhang, Guangtang Pan*. Genome expression profile analysis reveals important transcripts in maize roots responding to the stress of heavy metal Pb. Phyisologia Plantarum (IF=3.11), 2013,147（3）：270-28211. Yaou Shen, Zhou Jiang, Sifen Lu, Haijian Lin, Shibin Gao, Huanwei Peng, Guangsheng Yuan, Li Liu, Zhiming Zhan, Maojun Zhao, Tingzhao Rong, Guangtang Pan*. Combined small RNA and degradome sequencing reveals microRNA regulation during immature maize embryo dedifferentiation. BBRC (IF=2.46), 2013，441：425-430

12. Yaou Shen，Shujun Li，Zhou Jiang, Langlang Ma，Haijian Lin, Huanwei Peng，Li Liu，Guangsheng Yuan，Maojun Zhao，Shibin Gao，Zhiming Zhang，Guangtang Pan*. Overexpression of an Incw2 gene in endosperm improved yield-related traits in maize. Maydica (IF=0.4), 2012, 57: 147-153

13. Yaou Shen, Hongning Wang, Guangtang Pan*. Improving Inorganic Phosphorus Content in Maize Seeds by Introduction of Phytase Gene. Biotechnology, 2008, 7(2): 323-327

14. Yaou Shen, Yongzhong Zhang, Haijian Lin, Shibin Gao, Guangtang Pan*. Effect of low phosphorus stress on endogenous hormone levels of different maize genotypes in seedling stage. Journal of Biological Sciences, 2012,12(5):308-314

15. 沈亞歐，李淑君，林海建，張志明，江舟，何晶，潘光堂*.通過轉基因手段改善作物產量性狀. 農業生物技術學報，2011,19（4）：753-726

16. 沈亞歐，林海建，張志明，高世斌，潘光堂*. 植物逆境miRNA研究進展. 遺傳，2009，31(3): 227-235

17. 沈亞歐，彭煥偉，潘光堂*. 轉基因植物表達植酸酶的研究進展. 中國生物工程雜誌，2005，25（1）：25-32

18. Fei Ge, Maojun Zhao, Haijian Lin, Guangtang Pan and Yaou Shen*. Study on the Growth Inhibition Ratio of Rhizoctonia Solani Controlled by Nano-Al2O3/TiO2, Journal of Food Agriculture & Environment 2014(12)2: 1168-1171.(SCI, IF=0.5)

19. Junqiu Luo, Huanwei Peng, Yaou Shen*. Interorgan metabolism of nitrogen in nonruminants. Journal of Food, Agriculture & Environment, 2012, 10 (2): 598-602. (SCI, IF=0.5)

20. 馬浪浪，江舟，黃小波，沈亞歐*，潘光堂. 植物DNA甲基化調控研究進展. 中國生物工程雜誌，2013，33（9）：101-110

21. 吳花拉，張嚴玲，羅旭，葛飛，潘光堂，沈亞歐* . 位點特異性重組系統及其在植物轉基因研究中的應用. 中國生物工程雜誌，2014,34（11）：107-118

22. 楊珊, 王靜, 李清超, 劉麗, 張志明, 潘光堂, 沈亞歐*. 玉米自交系K10白色葉鞘遺傳機理研究. 植物遺傳資源學報，2014， 15（6）： 1167-1172

23. Hailan Liu, Yueai Lin, Guobo Chen, Yaou Shen, Jian Liu and Suzhi Zhang*. Genome-scale identification of resistance gene analogs and the development of their intron length polymorphism markers in maize. Molecular breeding, 2011, DOI: 10.1007/s11032-011-9560-3.(IF=2.0)

24. Fei Zheng, Guiqin Mu, Zhiming Zhang, Yaou Shen, Maojun Zhao, Guangtang Pan*. Nitrogen-doped hollow macroprous carbon spheres with high electrocatalytic activity for oxygen reduction. Materials Letters, 2011, doi:10.1016/j.matlet.2011.11.035 Key: citeulike:10047427.(IF=2.2)

25. Meng Xu, Yunsong Zhang, Zhiming Zhang, Yaou Shen, Maojun Zhao, Guangtang Pan*. Study on the adsorption of Ca2+. Cd2+ and Pb2+ by magnetic Fe3O4 yeast treated withEDTA dianhydride. Chemical Engineering Journal, 2011, 168:737-745. (IF=3.01).

26. Hongjun Liu, Xuerong Yang，et al., Yaou Shen, Haijian Lin, Thomas Lübberstedt, Zhiming Zhang, Guangtang Pan* .Genome-wide comparative analysis of digital gene expression tag profiles during maize ear development. Genomics, 2015, 106(1): 52-60

27. Fuqiang Yin, Cheng Qin, Jian Gao, Ming Liu, Xirong Luo, Wenyou Zhang, Hongjun Liu, Xinhui Liao, Yaou Shen, Likai Mao, Zhiming Zhang, Haijian Lin, Thomas Lübberstedt, and Guangtang Pan*. Genome-Wide Identification and Analysis of Drought-Responsive Genes and MicroRNAs in Tobacco. Int. J. Mol. Sci. 2015, 16, 5714-5740

28. Jian Gao, Yongzhong Zhang, Chaolong Lu, Hua Peng, Mao Luo, Gaoke Li, Yaou Shen, Haiping Ding, Zhiming Zhang, Guangtang Pan*, Haijian Lin*. The development dynamics of the maize root transcriptome responsive to heavy metal Pb pollution. Biochemical and Biophysical Research Communications, 2015, 458(2): 287-293

29. Haiping Ding, Jian Gao, Cheng Qin, Haixia Ma, Hong Huang, Pan Song, Xirong Luo, Haijian Lin, Yaou Shen, Guangtang Pan* and Zhiming Zhang*. The Dynamics of DNA Methylation in Maize Roots under Pb Stress. Int. J. Mol. Sci. 2014, 15, 23537-23554

30. Hongjun Liu, Cheng Qin, Zhe Chen, Tao Zuo, Xuerong Yang, Huangkai Zhou, Meng Xu, Shiliang Cao, Yaou Shen, Haijian Lin, Xiujing He, Yinchao Zhang, Lujiang Li, Haiping Ding, Thomas Lübberstedt, Zhiming Zhang*, Guangtang Pan*. Identification of miRNAs and their target genes in developing maize ears by combined small RNA and degradome sequencing. BMC Genomics, 2014, 15:25

31. Jian Gao, Zhe Chen, Mao Luo, Hua Peng, Haijian Lin, Cheng Qin, Guangsheng Yuan, Yaou Shen, Haiping Ding, Maojun Zhao, Guangtang Pan*, Zhiming Zhang*. Genome expression profile analysis of the maize sheath in response to inoculation to R. solani. Mol Biol Rep, 2014, 41:2471–2483

32. Haiping Ding, Cheng Qin, Xirong Luo, Lujiang Li, Zhe Chen, Hongjun Liu, Jian Gao, Haijian Lin, Yaou Shen, Maojun Zhao, Thomas Lübberstedt, Zhiming Zhang*, and Guangtang Pan*. Heterosis in early maize ear inflorescence development: A genome-wide transcription analysis for two maize inbred lines and their hybrid. Int J Mol Sci, 2014, 15, 13892-13915

33. Su Shunzong, Wu Ling, Liu Dan, Lu Yanli, Lin Haijian, Zhang Shuzhi, Shen Yaou, Liu Hailan, Zhang Zhiming, Rong Tingzhao, Zhang Xiao, Tian Yuehui, Nie Zhi, Gao Shibin*. Genome-wide expression profile of maize root response to phosphorus deficiency revealed by deep sequencing. Journal of Integrative Agriculture, 2014, 13(6), 1216-1229

34. Haiping Ding, Jian Gao, Mao Luo, Hua Peng, Haijian Lin, Guangsheng Yuan, Yaou Shen, Maojun Zhao, Guangtang Pan*, Zhiming Zhang*. Identification and functional analysis of miRNAs in developing kernels of a viviparous mutant in maize. The crop journal, 2013, 1(2): 115-126

35. Guangsheng Yuan, Zhiming Zhang, Kui Xiang, Yaou Shen, Juan Du, Haijian Lin & Li Liu, Maojun Zhao, Guangtang Pan*. Different Gene Expressions of Resistant and Susceptible Maize Inbreds in Response to Fusarium verticillioides Infection. Plant Mol Biol Rep, 2013, 31: 925–935

36. Guang-Sheng Yuan, Kui Xiang, Zhi-Ming Zhang, Ya-Ou Shen, Juan Du, Hai-Jian Lin, Li Liu and Guang-Tang Pan*. Analysis on the relationship between Fusarium verticillioides infection-induced genes and ear rot resistance in maize. Journal: Food, Agriculture and Environment, 2013, 11(2): 363-366

37. Zhiming Zhang, Jin Feng, Cui Wang, Jun Luo, Haijian Lin, Kui Xiang, Li Liu, Maojun Zhao, Yunsong Zhang, Haiping Ding, Shufeng Zhou, Yaou Shen and Guangtang Pan*. Difference between Pb and Cd Accumulation in 19 Elite Maize Inbred Lines and Application Prospects. Journal of Biomedicine and Biotechnology, 2012, 2012: 271485

38. Zhiming Zhang, Haijian Lin, Yaou Shen, Jian Gao, Kui Xiang, Li Liu, Haiping Ding, Guangsheng Yuan, Hai Lan, Shufeng Zhou, Maojun Zhao, Shibin Gao, Tingzhao Rong, Guangtang Pan*. Cloning and characterization of miRNAs from maize seedling roots under low phosphorus stress. Molecular biology report, 2012, 39: 8137-8146

39. Yuan Guangsheng, Zhang Zhiming†, Xiang Kui, Zhao Maojun, Shen Yaou, Pan Guangtang*. Large-scale identification of differentially expressed genes in maize inbreds susceptible and resistant to Fusarium ear rot. Plant omics journal, 2012, 9(5): 471-475

40. Hongjun Liu ,Huangkai Zhou ,Yongsheng Wu ,Xiao Li, Jing Zhao, Tao Zuo, Xuan Zhang, Yongzhong Zhang, Sisi Liu, Yaou Shen, Haijian Lin, Zhiming Zhang, Kaijian Huang,Thomas Lübberstedt,Guangtang Pan*. The Impact of Genetic Relationship and Linkage Disequilibrium on Genomic Selection. PLoS One, 10(7): e0132379