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趙華

四川農業大學動物營養研究所研究員

趙 華 博士,四川農業大學動物營養研究所研究員,博士生導師,四川省科學與技術帶頭人後備人選。美國普渡大學訪問學者。從事動物營養與分子生物學相關研究工作。先後主持或參研科研項目10餘項,其中包括主持國家自然科學基金面上項目4項,主持和主研國家“863”項目子課題各一項。已在J Nutr, PLOS One,Enzyme and microbiolgy technology及動物營養學報等國內外知名刊物上發表科研論文90餘篇,其中SCI論文50餘篇。申報國家發明專利4項,獲授權專利2項,專利號:ZL200510057408.3和ZL ZL201410124638.8.。2012年獲四川省科技進步二等獎1項(排名4)。

基本介紹


研究方向:飼料資源開發與利用、分子營養
一、學歷和工作簡歷:
教育經歷
2002/9 - 2006/12,重慶大學,生物醫學工程,博士
1999/9 - 2002/7,四川農業大學,動物營養與飼料科學,碩士
1993/9 - 1997/7,西南農業大學,動物營養與飼料加工,學士
工作經歷
2016/10-至今,四川農業大學,動物營養研究所,研究員
2009/12 - 2016/10,四川農業大學,動物營養研究所,副研究員
2013/09 - 2014/09,美國普渡大學,動物科學系,訪問學者
2007/4 - 2010/1,四川農業大學,動物營養研究所,助理研究員
2005/11 - 2007/4,四川農業大學,動物營養研究所,助教
二、教學課程:碩士研究生《飼料資源開發與利用》課程,本科生《飼料學》課程
三、指導研究生:畢業碩士研究生6人,在讀6人
四、當前主要研究工作:
1、國家自然科學基金面上項目“生長豬骨骼肌熱應激損傷中硒蛋白表達調控及其抗應激機制分析”(No:31772643),2018-2021,主持;
2、國家自然科學基金面上項目“日糧硒缺乏致雛雞胰腺萎縮差異基因表達分析及分子網路構建”,2013-2016,主持;
3、國家自然科學基金面上項目“應用siRNA技術結合基因置換和超表達探討豬selX基因生物學功能的研究”,2011-2011,主持;
4、國家自然科學基金面上項目“微型豬近交系肥、瘦表型腺體組織硒蛋白基因差異表達分析”,2009-2011,主持;
5、四川省教育廳重點項目“熱應激對腸道IPEC-J2細胞硒蛋白基因表達的影響”,2017-2019,主持;
6、國家863計劃子課題“豬胰腺消化酶基因表達活性分析及轉基因發酵生產”,2008-2010,主持;
7、國家863計劃子課題“抗菌肽基因表達活性分析及轉基因發酵生產”,2008-2010,第一主研;
8、四川農業大學雙支計劃;
9、企業合作項目。
五、代表性科研成果
Cao L, Tang JY, He AH, Jia G, Liu GM, Chen XL,Tian G, Cai JY, Shang HY,Zhao H*. SeleniumsupplementationalleviateHS-inducedIPEC-J2cellsinjuryvia regulation expression of severalselenoproteingenes. Under submission
Tang JY, He AH,Yan H, Jia G, Liu GM, Chen XL, Tian G,Cai JY, Shang HY,Zhao H*.2017.Damage to the myogenic differentiation of C2C12 Cells by heat stress is associated with up-regulation of several selenoproteins.submitted
Tang JY, He AH, Jia G, Liu GM, Chen XL, Cai JY, Shang HY, Liao JQ,Zhao H*.2017. Protective effect of selenoprotein X against oxidative stress-Induced cell apoptosis in human hepatocyte (LO2) cells via the p38 pathway. Bio Trac Elem Res.DOI: 10.1007/s12011-017-1025-z
Xu JY, Wang LQ, Tang JY, Liu GM, Chen XL, Cai JY, Shang HY,Zhao H*.2017. Pancreatic atrophy caused by dietary selenium deficiency induces hypoinsulinemic hyperglycemia via global down-regulation of selenoprotein encoding genes in broilers. PLoS ONE 12(8): e0182079.DOI: 10.1371/journal.pone.0182079
Tang JY, Huang XF, Li Q, Xu JY, Jia G, Liu GM, Chen XL, Shang HY, Cai JY,Zhao H*. 2017. Supranutritional dietary selenium depressed expression of selenoprotein genes in three immune organs of broiler. Animal Science Journal., 88:331-338. (SCI,IF=1.08)
Huang XF, Tang JY, Xu JY, Jia G, Liu GM, Chen XL, Shang HY, Cai JY,Zhao H*.2016. Supranutritional Dietary Selenium Induced Hyperinsulinmia and Dyslipidemia via Affected Expression of Selenoprotein Genes and Insulin Signal-related Genes in Broiler.Rsc Advances.6:84990-84998. (SCI,IF=3.840)
Tang JY, Cao L , Li Q, Xu JY, Jia G, Liu GM, Chen XL, Shang HY, Cai JY,Zhao H*.2016.Selenoprotein X gene knockdown aggravated H2O2-Induced apoptosis in liver LO2 cells. Bio Trac Elem Res.173:71-78.(SCI,IF=1.748)
Cao L, Tang JY, Li Q, Xu JY, Jia G, Liu GM, Chen XL, Shang HY, Cai JY,Zhao H*.2016.Expression of selenoprotein genes is affected by Heat stress in IPEC-J2 Cells. Bio Trac Elem Res. 172:354-360.(SCI,IF=1.748)
Zhao H*, Li K, Tang JY,Zhou JC, Wang KN, Xia XJ,Lei XG.2015 Expressions of selenoprotein genes is affected by obesity pigs fed a high-fat diet.J Nutr.145(7):1394-401. (SCI,IF=4.227)
Zhao H徠*, Tang JY,Cao L, Jia G, Long DB, Liu GM, Chen XL,Cai JY,Shang HY.2015.Characterization of bioactive recombinant antimicrobial peptide Parasin I fused with human lysozyme expressed in the yeast pichia pastoris system.Enzyme and microbiolgy technology.77:61-67. (SCI,IF=2.966)
Zhao H*, Tang JY, Xu JY, Cao L, Jia G, Long DB, Liu GM, Chen XL, Wang KN.2015.Selenoprotein Genes exhibited differential Expression pattern between hepatoma HepG2 cell and normal hepatocytes LO2 cell line. Bio Trac Elem Res. 167:236–241 (SCI,FI=1.608)
Zhao H*, Chen D, Tang JY, Jia G, Long DB, Liu GM, Chen XL, Shang HY. 2014. Partial optimization of the 5-terminal codon increased a recombination porcine pancreatic lipase (opPPL) expression in Pichia pastoris. Plos ONE. 9(12):e114385. (SCI,IF=3.533)
Sun LH, Li JG,Zhao H, Shi J, Huang JQ, Wang KN, Xia XJ, Li L and Lei XG.2013.Porcine Serum Can Be Biofortified with Selenium to Inhibit Proliferation of Three Types of Human Cancer Cells. J Nutr,143:1115-1122. (SCI,IF=4.196)
Liu Y,Zhao H*, Zhang QS, Tang JY, Li K, Xia XJ, Wang KN, Li K, and Lei XG.2012. Prolonged dietary Se deficiency or excess does not globally affect selenoprotein gene expression and/or protein production in various tissues of pigs.J.Nutr,142: 1410-1416. (SCI,IF=4.196)
Zeng MS, Li X, Liu Y,Zhao H, Zhou JC, Li K, Huang JQ, Sun LH, Tang JY, Xia XJ, Wang KN, Lei XG.2012. A high-selenium diet induced insulin resistance in gestating rats and their offspring. Free Radical Biology & Medicine, 52:1335-1342. (SCI,IF=5.223)
Huang JQ, Li DL,Zhao H, Sun LH, Xia XJ, Wang KN, Luo X, Lei XG.. 2011.The selenium deficiency disease exudative diathesis in chicks is associated with downregulation of seven common selenoprotein genes in liver and muscle. J Nutr,141:1605-10. (SCI,IF=3.96)
Li K,Zhao H,Zhou JC,Tang JY,Lei XG,Wang KN.2011.Differentially expressed genes in subcutaneous tissues in an obese pig models induced by a high-fat diets.Journal of animal and veterinary advances.10(14):1804-1810. (SCI)
Li JG, Zhou JC,Zhao H, Xia, XJ , Lei XG and Wang KN. 2011. Enhanced water-holding capacity of meat was associated with increased Sepw1 gene expression in pigs fed selenium-enriched yeast.Meat Science ,87:95-100. (SCI,IF=2.3)
Zhou JC,Zhao H,Li JG, Xia XJ, Wang KN, Zhang YJ, Liu Y, Zhao Y, and Lei XG. 2009.Selenoprotein gene expression in thyroid and pituitary of young pigs is not affected by dietary selenium deficiency or excess. J. Nutr, 139(6):1061-1066.(SCI,IF=3.96)
湯加勇,趙華*,賈剛,劉光芒,陳小玲,蔡景義,吳彩梅. 2016. Selx基因沉默及H2O2應激對LO2細胞中硒蛋白基因表達的影響。動物營養學報.28(8):2431-2438.
湯加勇,趙華*,何愛華,周繼昌,賈剛,劉光芒,陳小玲,蔡景義.2016.豬硒蛋白P基因克隆、鑒定及組織mRNA相對表達量分析. 動物營養學報, 28(3): 858-863.
趙華*,湯小朋,湯加勇,曹蕾,王雪濤,賈剛,劉光芒,陳小玲,王康寧.2016.複合生態菌固態發酵木薯渣工藝參數優化及混菌發酵對木薯渣營養品質的影響。中國畜牧雜誌.52(5):55-59
趙華*,王雪濤,湯加勇,湯小朋,賈 剛,劉光芒,陳小玲,龍定彪, 王康寧.2015.複合益生菌固態發酵改善甘薯渣營養價值的研究。動物營養學報, 27(4):1191-1198.
趙華*,王雪濤,湯加勇,湯小朋,賈 剛,劉光芒,陳小玲,龍定彪, 王康寧.2015.黑麴黴固態發酵甘薯渣條件優化及發酵對甘薯渣營養品質的影響。四川農業大學學報.33(1):51-56.
趙華*,湯加勇,曹蕾,周繼昌,賈剛,劉光芒,陳小玲,王康寧.2015.硒蛋白selX基因克隆、定點突變、原核表達及多克隆抗體製備。動物營養學報.27(5):1485-1491.
湯小朋,趙華*,湯加勇,王雪濤,賈 剛,劉光芒,陳小玲,龍定彪.2014. 黑麴黴固態發酵改善木薯渣品質的研究. 動物營養學報, 26(7):2026-2034.
趙華*,張艷艷,湯加勇,李珂,周繼昌,王康寧.2012.重組鰱魚抗菌肽parasinI原核表達、純化與抗菌活性. 動物營養學報, 24(9): 1731-1736.
專利:
1.申請獲得國家發明專利“一種酸性海藻糖酶及其製備技術”,專利號:ZL200510057408.3,專利公布號:CN1793348.第二申請人。
2.申請獲得國家發明專利“一種利用混菌發酵技術改善木薯渣品質的方法”,專利號:ZL201410124638.8.第一申請人。
3.申請國家發明專利“一種利用畢赤酵母高效分泌表達重組豬胰腺脂肪酶PPL的方法”,專利申請號:201410124242.1.第一申請人。
4.申請國家發明專利“一種利用畢赤酵母表達人溶菌酶-抗菌肽ParasinI融合蛋白的方法”,專利申請號:201510098860.8.第一申請人。
獲獎:
2012獲得四川省科技進步獎二等獎,排名第4,獲獎項目“豬雞飼料凈能評定及需要量研究與產業化應用”
六、獎勵和榮譽:
四川省科學與技術帶頭人後備人選