安利佳
中國遺傳學會理事
安利佳,男,現任中國遺傳學會理事,中國化工學會生物工程分會理事,教育部科委生命科學學部委員,遼寧省遺傳學會及生物技術學會理事。1998-2002年任大連理工大學生物工程系主任主要從事植物基因工程和藥物生物技術研究,先後主持國家科技攻關項目1項、省、市級重大研究課題4項。
1982年2月於遼寧師範學院獲理學學士學位
1989年6月於東北師範大學植物學專業獲理學博士學位。
1984年10月至1998年3月在遼寧師範大學任教,1996年晉陞教授。
1995年獲遼寧省優秀青年科技工作者稱號。
1996年獲遼寧省首屆青年科研人才基金。
1999年獲大連市優秀人才獎。
2001年獲大連市優秀專家稱號。
2001年被評為遼寧省第二批百千萬人才工程百人層次人選。
2004年擔任遼寧省生物技術協會專家委員會專家
1. 植物轉基因工程與育種技術平台研究。編號:JY03-B-18,國家科技部植物轉基因重大專項,100萬元,2003-2004
2. 基因工程關鍵技術。項目編號:2001101001,遼寧省科技廳,210萬元,2001-2004
4. 蛇島蝮蛇類凝血酶基因的分離和表達。遼寧省科技攻關,10萬元,1999.01-2001.12
5. 蛇島蝮蛇類凝血酶基因的分離和表達。大連市科委,15萬元,1998-2001
6. 胸腺素α1基因工程藥物開發。通化金馬製藥股份公司,390萬元,1998.01~2001.12
8. 長春花鹼單體的分離研究。大連理工大學領先集團有限公司,20萬元,2002.7-2003.7
9. 真福康保健功效的驗證與鑒定。大連理工大學領先集團有限公司,10萬元,2002.7-2003.7
指導碩、博士生研究方向:植物基因工程、生物製藥
五年來共發表文章120餘篇,SCI收錄42篇,主要文章包括:
1. Properties of A. ficuum AS3.324 phytase expressed in tobacco. Process Biochemistry,2005,40:213–216 SCI
2. Purification,characterization and gene cloning of a novel phospholipase A2 from the venom of Agkistrodon blomhoffii ussurensis. The International Journal of Biochemical & Cell Biology. 2005,37:558-565 SCI
3. Neuroprotective properties of catalpol in transient global cerebral ischemia in gerbils: dose-response,therapeutic time-window and long-term efficacy. Brain Research,2004,1029:179-185 SCI
4. Plasmodium falciparum histone acetyltransferase,a yeast GCN5 homologue involved in chromatin remodeling. Eukaryotic Cell,2004,3(2):264-276
5. Catalpol inhibits apoptosis in hydrogen peroxide-induced PC12 cells by preventing cytochrome c release and inactivating of caspase casecade. Toxicon,2004,43:53-59
6. PfADA2,a Plasmodium falciparum homologue of the transcriptional coactivator ADA2 and its in vivo association with the histone acetyltransferase PfGCN5. Gene,2004,336:251-261
7. Construction of Targeting Vector and Expression of Green Fluorescent Protein in the Silkworm,Antheraea pernyi. DNA and Cell Biology. 2003,22:441-446
8. The Expression of GFP Under the Control of Fibroin Promotor in Primary ovarian cells of Antheraea pernyi. Journal of Biosciences,2003,28(6):691-695
10. Codonopsis pilosula (Franch) Nannf total alkaloids potentiate neurite outgrowth induced by nerve growth factor in PC12 cells. Acta Pharmacologica Sinica,2003,24(9):913-917
11. Effect of Cuscuta chinensis glycoside on the neuronal differentiation of rat pheochromocytoma PC12 cells. International Journal of Developmental Neuroscience,2003,21:277-281
12. Hydrogen peroxide-induced apoptosis in PC12 cells and the protective effect of puerarin. Cell Biology International,2003,27:1025-1031
13. Neuroprotective effect of Alpinia oxyphylla Miq. fruits against glutamate-induced apoptosis in cortical neurons. Toxicology Letters,2003,144(2):205–212
14. Neuroprotective effect of Alpinia oxyphylla extract against glutamate excitotoxicity in cultured mouse cortical neurons. Neurosci Res Commun,2003,33(2):105–113
15. Investigation of the neuronal death mode induced by glutamate treatment in serum-,antioxidant-free primary cultured cortical neurons. Developmental Brain Research,2003,145(2):263-268
16. A new way for chemical degradation of plastic by natural volatile constituents of Ledum palustre. Chinese Science Bulletin,2003,48(16):1718-1721
17. Enhancing ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae by Mg2+ via reduction in plasma membrane permeability. Biotechnology Letters,2003,25:1191-1194
18. Dextromethorphan protects dopaminergic neurons against inflammation-mediated degeneration through inhibition of microglial activation. The Journal Of Pharmacology and Experimental Therapeutics,2003,305(1):212-218
19. 酸性水溶液中硫氰酸鉬(V)配合物的研究。光譜學與光譜分析,2003,23(2)337-339
20. Purification and characterization of ulva pertusa kjellm alkaline phosphatase. Preparative Biochemistry & Biotechnology,2003,33(2):113-123
21. Cloning of cDNA Encoding Choline Monooxygenase from Suaeda liaotungensis and Salt Tolerance of Transgenic Tobacco. Acta Botanica Sinica,2003,45(2):242-247
22. Molecular cloning and characterization of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis and its use in improved tolerance to salinity in transgenic tobacco. Biotechnol Lett. 2003,25(17):1431-1436
23. Characterization of Sporothric schenckii by random amplication of polymorphic DNA assay. Chinese Medicine Journal,2003,116(2):239-242
24. Expression of gloshedobin,a thrombin-like enzyme from the venom of Gloydius shedaoensis,in Escherichia coli. Biotechnology Letters,2003,25(2):101–104
25. High-level expression of a soluble snake venom enzyme,gloshedobin,in E. coli in the presence of metal ions. Biotechnology Letters,2003,25(8):607–610
26. Purification and characterization of ginsenoside--arabinofuranase hydrolyzing ginsenoside Rc into Rd from the fresh root of Panax ginseng. Process Biochemistry,2002,37:793-798
27. Cloning and Expression of defibrase cDNA from the venom of Gloydius shedaoensis. Biotechnology Letters,2002,24:135-138
28. Cloning Expression and Purification of Gussrobin A Thrombin-like Enzyme from the Snake Venom of Gloydius ussuriensis. Prog Biochem Biophys. 2002,34(1):6-10
29. Expression,Purification and Partial Characterization of Recombinant Defibrase,a Thrombin-Like Enzyme from the Venom of Glyodius shedaoensis. Prog Biochem Biophys. 2002,29(3)390-393
30. Production of a Functional Single-chain Fv Protein in Transgenic Tobacco Root Exudates. Biotechnology letters. 2002,24(18):1531-1534
31. An expeditious method for constructing T-vectors using Eam 1105 Ⅰ cassettes. Plant Molecular Biology Reporter,2002,20:189a-189e
32. Application of DNA sequencing in detection of telomerase activity. Biochemical Engineering Journal. 2002,11(2-3):79-85
33. Production of high affinity human single-chain antibody against preS1 of Hepatitis B Virus: comparison of large naïve and in vitro immune phage displayed antibody library. Progressing in Biochemistry and Biophysics. 2002,29(4):572-575
34. Inhibition by naloxone stereoisomers of beta-amyloid peptide (1-42)-induced superoxide production in microglia and degeneration of cortical and mesencephalic neurons. Journal of Pharmacology and Experimental Therapeutics,2002,302(3):1212-1219
35. A Serum- and Antioxidant-free Primary Culture Model of Mouse Cortical Neurons for Pharmacological Screen and Studies of Neurotrophic and Neuroprotective Agents. Cellular and Molecular Neurobiology. 2002,22(2):197-206
36. Expression of endoplasmic reticulum molecular chaperon GRP94 in human lung cancer tissues and its clinical significance. Chinese Medical Journal,2002,115(11):1615-1619
37. Embryonic stem cell as nuclear donor could promote in vitro development of the heterogeneous reconstructed embryo. Chinese Science Bulletin,2002,47(21):1811-1815
38. 銅鋅超氧化物歧化酶與色氨酸鈷(Ⅱ)的相互作用。光譜學與光譜分析,2000,3:305-307
40. Kinetics of Inactivation of Ulva pertusa Kjellm Alkaline Phosphatase by Ethylenediaminetetraacetic Acid Disodium. Journal of Enzyme Inhibition,2001,16:313-319
41. Purification and Characterization of ginsenoside-?-Glucosidase from Ginseng. Chem. Pharm. Bull,2001,49(7):795-798
42. 分光光度法研究EDTA對小牛腸鹼性磷酸酶的不可逆抑制動力。光譜學與光譜分析,2001,21(5):701-703
43. 鹼性磷酸梅與Cu (Ⅱ)相互作用的譜學研究。光譜學與光譜分析,2001,21(4):432-434
44. 氨基醯化酶與Cu(Ⅱ)相互作用的譜學研究。光譜學與光譜分析,2001,21(6):804-806
47. 新的重組蛋白表達系統——植物根分泌和葉分泌。生物化學與生物物理進展,2003,30(1):128
49. Variation and characterization analysis of partial fragment of fibroin gene from silkworm,Antheraea pernyi. High Technology Letters,2003,9(3):29-32
50. 喜樹鹼生物合成途徑及其代謝調控的研究進展。高技術通訊,2004,14(5):106-110
51. 菟絲子提取物對PC12細胞中GAP-43表達影響。大連理工大學學報,2004,44(3):378-382
52. Rapid Amplification of 5′cDNA End of Suaeda liaotungensis Choline Monooxygenase Using Inverse PCR RACE. High Technol Lett 2002,8(1):5-7
53. Separation and purification of betaine aldehyde dehydrogenase from wild Suaeda liaotungensis. High Technology Letters,2002,8(2):5-17
54. Cloning a Full-length cDNA Encoding UDP-glucose Pyrophosphorylase from Amorpha Fruticosa by PCR-Based Methods. High Technology Letters,2002,8(3):5-10
55. An Lijia. A co-expression system based on phage and phagemid to select cognate antibody-antigen pairs in vivo. High Technology Letters,2002,8(2):5-10
56. Construction of Large Human Single-chain Antibody Phage Display Library. High Technology Letters,2002,8(3):1-4
57. 精子幹細胞轉染法製備轉基因兔的研究。高技術通訊,2001,11(130):17-21
58. 反向嵌套PCR高效擴增遼寧鹼蓬甜菜鹼醛脫氫酶cDNA 5’末端序列。高技術通訊,2001,1(11):17-19
59. Research on telomerase detection of activity by combining DNA sequence analysis with TRAP. High Technology Letters,2001,7(3):8-10
61. Separation and Purification of Thrombin-like Enzyme by Affinity Adsorbents. High Technology Letters,2001,7(4):13-15
62. 卵黃免疫球蛋白配基親和純化鹼性磷酸酶。中國農業科學,2004,37(6):908-911
英漢生物技術辭彙
作者:安利佳等 ISBN:750253978
出版社:化學工業出版社 出版日期:2003-01-01
共承擔國家、省、市課題10項,項目經費總計900萬元;
申報國內外專利13項,其中國際專利1項;
由安利佳教授為負責人完成的“一種植物分子育種新技術的建立與應用”項目鑒定會,於2004年12月19日通過了遼寧省科技廳科技成果鑒定,達到了國際領先水平。
所主持的遼寧省科技廳項目“基因工程關鍵技術”和大連市科技局項目“無載體、無標記基因植物轉基因技術”於2004年4月6日通過了遼寧省科技廳科技成果驗收和大連市科技局科技成果鑒定,達到了國際先進水平。
2004年安利佳教授率領的課題組被學校評為大連理工大學科研創新團隊。