孔令讓

1988－1990，助教，山東農業大學農學系。

1990－1993，講師，山東農業大學農學系。

1996－1997，副教授，山東農業大學農學系。

1997－1998，博士后，德國墨尼黑技術大學植物育種系。

1998－1999，副教授，山東農業大學農學系“植物遺傳工程”省重點實驗室。

1999－2000，教授，山東農業大學農學系“植物遺傳工程”省重點實驗室主任，教務處副處長。

2000.5－2000.9，博士后，以色列國海法大學進化研究所。

2000－2003，博士后，美國普度大學農學系。

2003－2008，研究遺傳學家，美國普度大學農學系。

2008－，山東農業大學遺傳育種學科特聘教授，“作物生物學”國家重點實驗室常務副主任。

現任山東省小麥分子育種學崗位“泰山學者”特聘教授，中國遺傳學會理事，山東遺傳學會副理事長兼秘書長，《作物學報》常務編委，《The Crop Journal》編委。

研究項目

1990－1995，小麥－偃麥草二體異附加系的選育，山東省科委。

1992－1997，利用小麥－偃麥草異附加系選育優質、多抗冬小麥新品系的研究，山東省科委。

1997－2000，小麥和粗山羊草正反交育性的分子機理研究，山東省自然科學基金。

1999－2001，小麥抗白粉病基因的標記及克隆，國家教育部留學回國人員基金。

1999－2002，利用粗山羊草豐富小麥D染色體組遺傳多樣性的研究，國家自然科學基金（NSFC）。

2000－2005，將基因組學引入小麥育種的研究，美國農業部。

2001－2008，利用分子標記輔助選擇培育軟粒、抗赤霉病冬小麥的研究，美國農業部。

2005－2008，偃麥草抗赤霉病基因的鑒定及其遺傳轉移，美國農業部。

2006－2008，利用基因沉默鑒定小麥抗赤霉病基因的研究，美國農業部。

2006－2009，小麥抗病基因QTL標記及分子育種研究，美國農業部。

2020年，山東農業大學農學院教授、山東省現代農業產業技術體系小麥創新團隊首席專家孔令讓及其團隊從小麥近緣植物長穗偃麥草中首次克隆出抗赤霉病主效基因Fhb7，且成功將其轉移至小麥品種中，首次明確並驗證了其在小麥抗病育種中不僅具有穩定的赤霉病抗性，而且具有廣譜的解毒功能。研究成果4月10日在線發表於《科學》。

近來主要研究領域

Wheat genetics and breeding with an emphasis on wheat breeding and germplasm enhancement using chromosome engineering and molecular marker-assisted selection; marker-assisted backcrossing; genetics of plant disease and pest resistances; gene mapping (including QTLs); genetics of wheat soft grain texture; and genetics of plant-pathogen interactions.

1) Applying genomic approaches for wheat improvement

The combined use of conventional breeding with MAS, genetics, and bioinformatics accelerated the development of new wheat cultivars with enhanced yield, grain quality and disease/insect resistances (FHB, BYDV, Sr, Lr, Yr, Hessian fly, and so on). Selection efficiency had been significantly increased by using markers-trait associations.

2) Understanding the mechanisms of wheat–pathogens interactions:

Induction of wheat defense and stress-related genes in response to Fusarium graminearum; Identification of differentially expressed genes corresponding to FHB resistance using SSH, GeneCalling and a cDNA library; Selection of candidate genes for specific plant defense; Verification of the integrated network for resistance to pathogens in the plant-pathogen interactions; Analysis of FHB resistance in wheat using the Virus-Induced Gene Silencing (VIGS) system.

3) Genetic study on plant resistance gene

Amplified Fragment Length Polymorphism (AFLP) and Restriction Fragment Length Polymorphism (RFLP) mapping for powdery mildew resistance gene (Pm29) and microsatellite (SSR) mapping for yellow rust resistance gene (Yr15); Identification of QTLs controlling low FHB incidence in wheat and determination of the location of Striga gesnerioides resistance in cowpea (Vigna unguiculata).

4) Applying genomic approaches for wheat germplasm enhancement

Marker-assisted selection in wheat breeding and germplasm enhancement; Improvement of Triticum aestivum D genome diversity exploiting Aegilops tauschii D genome; Improvement of soft winter wheat for resistance to Fusarium head blight; Bring genomics into wheat fields.

5) Introgressing alien interest genes into adapted wheat lines

Gene introgressions for rust resistance, powdery mildew resistances and quality traits; Developing and releasing an excellent cultivar ‘Shannong 408698’ with leaf rust resistance and quality traits; Identification of FHB resistance genes from Thinopyron elongatum (E, 2n=14) and Leymus multicaulis (XN, 2n=28); Pyramiding barley yellow dwarf virus (BYDV) resistance genes Bdv2 and Bdv3 into one genotype using ph mutant and null-tetrasomic lines and marker-assisted selection.

6) Expanding the current range of softer wheat textures by introgressing additional active genes from T. monococcum

Selection of translocation lines with shortened T. monococcum fragment carrying Pina and Pinb using Pina-Am1, Pinb-Am1 and Gsp-Am1 allele-specific DNA makers and ph mutant; Construction of a series of backcrossing segregation populations (BC1F1) of Ham using crosses between Chinese Spring-T. monococcum translocation (CS - 5Am) and wheat cultivars differing in grain textures; Effect of additional T. monococcum active softness genes on wheat grain textures using the molecular markers; Development of wheat varieties with much softer grain textures than soft wheat.

7) Crown rust and yellow dwarf virus resistance gene mapping in oats

Genetics of crown rust and YDV resistance genes in oats (Avena sativa); Introgression of YDV resistances from wild diploid into cultivated oats by embryo rescue.

著作

1．副主編，冬小麥“矮孟牛”種質的創製，山東農業科技出版社，2000

2．參編，生物技術在農業上的應用，中國教育出版社，1999

發表或待發表論文

1) L. Kong, J. M. Anderson, and H. W. Ohm. 2007. A network integration of NBS-LRR resistance genes and pathogen-response (PR) genes involved in the plant-pathogen interaction. (in preparation)

2) L. Kong, J. M. Anderson, and H. W. Ohm. 2007. Identification of disease resistances in wheat-Leymus multicaulis derivatives and characterization of L. multicaulis chromatin using microsatellite DNA markers. Genome (submitted).

3) X. Shen, L. Kong, H. Sharma, and H. W. Ohm. 2007. Marker-assisted reduction of a chromosome segment of Thinopyrum ponticum carrying Fasurium head blight resistance. Plant Breeding (submitted).

4) L. Kong, J. M. Anderson, and H. W. Ohm. 2007. Segregation distortion in bread wheat of Thinopyrum intermedium 7E segment carrying Bdv3. Plant Breeding (submitted).

5) L. Kong, H. W. Ohm, and J. M. Anderson. 2007. Expression analysis of defense-related genes in wheat in response to infection by Fusarium graminearum. Genome. 50: 1038-1048.

6) L. Kong, S. E. Cambron, and H. W. Ohm. 2007. Hessian fly resistance genes H16 and H17 are mapped to a resistance gene cluster in the distal region of chromosome 1AS in wheat. Molecular Breeding. DOI 10.1007/s11032-007-9119-5.

7) M. Bonafede§, L. Kong§ (§ Two first authors contributed equally to this research), G. Tranquilli, H. Ohm, and J. Dubcovsky. 2007. Reduction of a Triticum monococcum chromosome segment carrying the softness genes Pina and Pinb translocated to bread wheat. Crop Science 47: 821-826.

8) L. Kong, J. M. Anderson, and H. W. Ohm. 2005. Induction of wheat defense and stress-related genes in response to Fusarium graminearum. Genome. 48: 29-40.

9) L. Kong, H. Ohm, S. Cambron, and C. Williams. 2005. Molecular mapping determines that Hessian fly resistance gene H9 is located on chromosome 1AS of wheat. Plant Breeding. 124: 525-531.

10) J. Gilsinger, L. Kong, X. Shen, and H. Ohm. 2004. DNA markers associated with low Fusarium head blight incidence and narrow flower opening in wheat. Theor. Appl. Genet. 110: 1218-1225.

11) O. Boukar, L. Kong, B.B. Singh, L. Murdock, and H. Ohm. 2004. AFLP markers and AFLP-derived SCAR marker associated with Striga gesnerioides resistance in cowpea. (Vigna unguiculata (L.) Walp). Crop Science. Crop Science. 44: 1259-1264.

12) X. Shen, L. Kong, and Herbert Ohm. 2004. Fusarium Head Blight resistance in hexaploid wheat (Triticum aestivum)-Lophopyrum genetic lines and tagging of alien chromatin by PCR markers. Theor. Appl. Genet. 108: 808-813.

13) F.J. Zeller, L. Kong, L. Hartl, V. Mohler and S.L.K. Hsam. 2002. Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.) 7. Gene Pm29 in line Pova. Euphytica 123:187-194.

14) C. Li, Y. Liu, C. Du, and L. Kong. 2002. Embryological studies on the fertility of the original and reciprocal crosses between T. aestivum and Ae. Sqarrosa. Acta Agronomica Sinuca. 28(2):170-174.

15) L. Kong, Y. Dong, H. Zhang, C. Ma, and H. Wang. 2000. Studies on the fertility of original cross and reciprocal cross between T. aestivum and Ae. tauschii. Acta Botanica Boreali-occidendalia Sinica. 20(4): 519-523.

16) D. Fu, L. Kong, Y. Xiao, X. Zhang, and X. Yang. 2000. Production and cytological study of fertility of intergeneric hybrids of T. aestivum with Ae. vavilovii. Acta Botanica Boreali-occidendalia Sinica. 20(4): 511-518.

17) L. Kong, Y. Dong, and J. Jia. 1999. Location of powdery mildew resistance gene in Am6, Amphidiploid between Triticum durum and Aegilops sqarrosa, and its utilization. Acta Phytophylacica Sinica. 26(2): 116-120.

18) L. Kong, and Y. Dong. 1999. Creation of new resistance amphidiploid between Triticum carthlicum and Aegilops sqarrosa. Acta Botanica Boreali-occidendalia Sinica. 19(2):196-199.

19) L. Kong, Y. Dong and J. Jia. 1998. Random Amplified Polymorphism of DNA analysis in Aegilops sqarrosa. Acta Botanica Sinica. 40(3):223-227.

20) L. Kong and Y. Dong. 1998. Preliminary studies on cytogenetics of progenies between Triticum aestivum L. and Aegilops sqarrosa L. Acta Biologiae Experimentalis Sinica. 31(2):209-213.

21) S. Liu, J. Jia, H. Wang, and L. Kong. 1999. Homoelogy identification between Elytrigia elongatum (2n=14) and wheat chromosomes by using biochemical and molecular markers. Chinese Journal of Genetics. 26(1);75-81.

22) S. Liu, H. Wang, and L. Kong. 1998. Special chromosome marker for E genome and DNA polymorphism between Agropyron elongatum (2n=14) and common wheat detected by RAPD markers. Acta Agronomic Sinica. 24(6):685-690.

23) S. Liu, H. Wang, and L. Kong. 1998. Polymorphism detection between wheat and Agropyron elongatum (2n=14) by using molecular markers. Acta Botanica Borealis-Occidendalia Sinica. 18(5):7-10.

24) S. Liu, H. Wang, Z. Qi, F. Kong, and L. Kong. 1998. Studies on transmission characteristics of alien chromosomes in hybrid progenies between octoploid Agrotriticum, Tritileymus and Triticum aestivum. Acta Botanica-Occidendalia Sinica. 18(5):122-126.

25) L. Kong, and Y. Dong. 1997. Studies on cytogenetic diversity of powdery mildew resistant genes from Aegilops sqarrosa. Acta Agronomica Sinica. 23(2):176-180.

26) L. Kong, and Y. Dong. 1997. Studies on cytogenetics of progenies between Triticum aestivum L. and Amphidiploid from Triticum durum-Aegilops sqarrosa. Acta Agronomica Sinica. 23(4):505-508.

27) L. Kong, Y. Dong, and J. Jia. 1997. Chromosome configurations and fertility characteristics of hybrids between Triticum aestivum L. and Aegilops sqarrosa L. Acta Biogiae Experimentalis Sinica. 30(1);35-43.

28) C. Li, L. Kong and, X. Kong. 1996. Primary selection of RAPD marker from U and C genomes of goatgrass (Aegilops). Journal of Shandong Biotechnology. First Issue:17-23.

29) H. Wang, L. Kong, and L. Jiang. 1996. Segregation of chromosome and traits in self-bred and backgrossed progenies of hybrids between octoploid Tritilytrigia and Triticum aestivum. Acta Agronomica Sinica. 22(4):508-512.

30) L. Li, X. Li, and L. Kong. 1996. Agropyron plants distributed in Xilingol grassland. Crop Genetic Resources. 4:9-11.

31) L. Kong, F. Guo, and H. Wang. 1993. Cytoembryological studies on low fertility in hybrids between octoploid Agrotriticum and Triticum aestivum L. Proc. Int. Wheat Genet. Symp. 8th Beijing, P. R. China. Vol. 1: 261-265.

32) H. Wang, D. Zhang, and L. Kong. 1993. Anther culture studies on hybrid progenies between octoploid Tritilytrigia and Triticum aestivum. Acta Botanica Borealis-Occidendalia Sinica 13(3): 186-191.

33) L. Kong. 1992. Cytogenetic studies on offspring of octoploid Agrotriticum × Triticum aestivum L. Chinese Journal of Genetics 19(4): 317-323.

34) H. Wang, L. Kong, and L. Jiang. 1992. A method for increasing seed setting frequency of T. aestivum × E. elongata. Journal of Shandong Agricultural University 23(3): 265-270.

35) Z. Ruan, L. Kong, and Y. Wei. 1992. Comparative studies on pollen cytomorphology of male sterile and male fertile in Allium fistulosum L. Journal of Shandong Agricultural University 23(1): 60-66.

36) L. Kong, H. Wang, and F. Guo. 1991. Cytological observation of microsporogenesis and pollen development in octoploid Agrotriticum, T. aestivum and their hybrids. Acta Botanica Sinica 33(3): 603-609.

37) F. Guo, L. Kong, and H. Wang. 1992. An embryological study on the low fertility of F1 plants from T. trititrigia × T. aestivum L. Scientia Agricultura Sinica 25(5): 20-3.

38) L. Kong. 1990. Tendency of chromosome segregation of offspring between octoploid Agrotriticum and T. aestivum. Journal of Shandong Agricultural University 21(4): 1-7.

39) L. Kong, and Y. Dong. 1999. Advances on the genetic diversity of Aegilops sqarrosa. Journal of Shandong Agricultural University 30(4): 464-470.

40) L. Kong, and Y. Dong. 1998. Progress in effective utilization of Aegilops sqarrosa. Journal of Shandong Agricultural University 29(4): 543-548.

41) Y. Yu, L. Kong, and D. Zhang. 1990. Isolation of nuclei and studies on nuclear DNA part characeristecs in Allium sativum L. Journal of Shandong Agricultural University 21(2): 54-58.

42) H. Wang, J. Zhao, L. Jiang, and L. Kong. 1989. Cytological and main traits inheritance of the ten man-made superior wheat germplasm lines. Journal of Shandong Agricultural University 19(2): 1-7.

美國國內或國際會議論文/牆報/會議報告

1) C.Zila, L. Kong, H. W. Ohm. 2007. Molecular mapping of Fusarium head blight resistance of common wheat Xing117. Nov. 4-8. New Orleans, MI.

2) L. Kong, J. M. Anderson, and H. W. Ohm. 2007. Expression analysis of defense-related genes in wheat in response to infection by Fusarium graminearum using GeneCalling. Nov. 4-8. New Orleans, MI.

3) H. W. Ohm, L. Kong, and X. Shen. 2007. Backcrossing Multiple Novel Genes/Traits into Adapted Wheat Backgrounds by Marker-Assisted Selection. March 23-27. Sanya, Hainan, China.

4) L. Kong, J. M. Anderson and H. W. Ohm. 2007. Segregatiuon distortion in bread wheat of Thinopyrum intermedium 7E segment carrying Bdv3. Poster# 276. The International Conference on the Status of Plant & Animal Genome Research XV. January 13-17. San Diego, CA.

5) S. R. Scofield, C. Cakir, A. S. Brandt, L. Kong, and J. M. Anderson. 2007. VIRUS-induced gene silencing in hexaploid wheat. Poster# 794. The International Conference on the Status of Plant & Animal Genome Research XV. January 13-17. San Diego, CA.

6) L. Kong, J. Anderson, and H. Ohm. 2006. Development of a co-dominant marker diagnostic for yellow dwarf virus resistance transferred from Thinopyrum intermedium into common wheat. June, 2006. Wheat Field Trip Day, Purdue Agronomy Research Center, West Lafayatte, IN.

7) L. Kong, M. Bonafede, J. Dubcovsky, and H. Ohm. Development of STS markers linked to grain texture and a co-dominant marker for the adjacent BGGP gene to Gsp from Triticum monococcum. The International Conference on the Status of Plant & Animal Genome Research XIV, Poster #120. January 14-18. San Diego, CA.

8) X. Shen, L. Kong, H. Sharma, and H. Ohm. Development and characterization of a wheat translocation line with Fusarium head blight resistance derived from Thinopyrum Ponticum. Proceedings of the 2006 National Fusarium Head Blight Forum. Poster #67. December 10-12. Ralergh, NC.

9) S. Scofield, A. Brandt, C. Cakir, L. Kong, and J. Anderson. 2006. VIRUS-induced gene silencing in hexaploid wheat. Proceedings of the 2006 National Fusarium Head Blight Forum. Poster #58. December 10-12. Ralergh, NC.

10) L. Kong, M. Bonafede, J. Dubcovsky, and H. Ohm. 2006. DNA markers linked to the grain texture locus in diploid wheat. Poster #120. January 14-18. San Diego, CA.

11) M. Bonafede, L. Kong, G. Tranquilli, H. Ohm, and J. Dubcovsky. Reduction of a Triticum monoccum chromosome segment carrying the softness genes Pina and Pinb translocated to bread wheat. Poster #319. January 14-18. San Diego, CA.

12) X. Shen, H. Sharma, L. Kong, and H. Ohm. 2006. Development of chromosome recombinant line of wheat-Thinopyrum ponticum with resistance to Fusarium head blight. Poster #308. January 14-18. San Diego, CA.

13) X. Shen, H. Sharma, L. Kong, and H. Ohm. 2005. Molecular characterization of a chromosome recombinant carrying a FHB QTL from Lophopyrum ponticum. Proceedings of the 2005 National Fusarium Head Blight Forum. Poster #38. December 11-13. Milwaukee, WI.

14) H. Sharma, L. Kong, X. Shen, and H. Ohm. 2005. Shortening the chromosome 7el2 segment from Thinopyrum Ponticum in the wheat translocation line KS24-2. Poster #238-4. ASA-CSSA-SSSA. Nov. 6-10, 2005, Salt Lake City, UT.

15) L. Kong, J. Dubcovsky, and H. Ohm. 2005. STS markers linked to grain texture in diploid wheat, and Marker-Assisted Selection to shorten the segment of T. monococcum chromosome 5Am conferring soft-textured grain using Ph mutant. Eastern and Southern Wheat Breeding Conference, 9-12 May, 2005. Bowling Green, KY.

16) L. Kong, H. Ohm, and J. Anderson. 2005. Expression analysis of defense-related genes in wheat in response to infection by Fusarium graminearum. Proceedings of the 2005 National Fusarium Head Blight Forum. Poster #74. December 11-13. Milwaukee, WI.

17) L. Kong, J. Anderson, and H. Ohm. 2005. Distorted transmission of a wheatgrass translocation for YDV resistance and marker-assisted backcrossing into wheat. Plant & Animal Genome XII Conference, San Diego, California, USA, January 15-19.

18) L. Kong, J. M. Anderson, and H. W. Ohm. 2004. Identification and characterization of defense response genes from wheat differentially induced by infection with Fusarium graminearum. Plant & Animal Genome XII Conference, San Diego, California, USA, p111.

19) J. Gilsinger, L. Kong, X. Shen, and H. Ohm. 2004. DNA markers linked to narrow flower opening and low FHB incidence in wheat. 2nd International Symposium on Fusarium Seminar, Orlando, Florida, USA.

20) L. Kong, X. Shen, J. Uphaus, J. Gilsinger, M. Giovanini, H. Sharma, and H. Ohm. 2004. Development of DNA markers and marker-assisted selection in wheat breeding. Soft Winter Wheat Field Trip of Eastern U.S.A., West Lafayette, IN, USA, June 15, 2004.

21) J. Gilsinger, L. Kong, X. Shen, and H. Ohm. 2004. DNA markers linked to flower opening and low FHB incidence in wheat. Soft Winter Wheat Field Trip of Eastern U.S.A., West Lafayette, IN, USA, June 15, 2004.

22) X. Shen, L. Kong, and H. Ohm. 2004. Pyramiding FHB resistance derived from wheatgrass with QTLs from wheat. Soft Winter Wheat Field Trip of Eastern U.S.A., West Lafayette, IN, USA, June 15, 2004.

23) X. Shen, L. Kong, and H. W. Ohm. 2004. Marker-assisted characterization of Fusarium head blight resistance in wheat derived from wheatgrass. Plant & Animal Genome XII Conference, San Diego, California, USA, p183.

24) L. Kong, J. M. Anderson, and H. Ohm. 2003. Identification of scab resistance gene expression in wheat following inoculation with Fusarium. Plant & Animal Genome XI Conference, San Diego, California, USA, p808.

25) L. Kong, J. M. Anderson, and H. W. Ohm. 2002. Identification of scab resistance gene expression in wheat following inoculation with Fusarium. 2002 National Fusarium Head Blight Forum Proceedings, Erlanger, KY, USA, p30.

26) L. Kong, J. M. Anderson, and H. W. Ohm. 2003. Identification and characterization of defense response genes from wheat differentially induced by infection with Fusarium graminearum. Annual Meetings for American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Denver, CO, USA, p227.

27) H. Ohm, L. Kong, X. Shen, and J. Uphaus. 2003. Marker-assisted backcrossing of traits into soft winter wheat. Southern Small Grains Workers Conference. Little Rock, AR, USA. April 27-29.

28) X. Shen, L. Kong, and Herbert W. Ohm. 2002. Novel source of type II resistance to Fusarium Head Blight. 2002 National Fusarium Head Blight Forum Proceedings, Erlanger, KY, USA, p212.

29) J. Peng, A. Korol, T. Fahima, J, Cheng, L. Kong, M.S. Roder, Y.I. Ronin, and E. Nevo. 2001. Massive negative interference, and putative quasi-linkage upon hybridization of wild emmer wheat, Triticum dicoccoides, with cultivated wheat, T. durum. Plant & Animal Genome IX Conference, San Diego, California, USA. p432.

30) X. Shen, L. Kong, and H. Ohm. 2002. Fusarium head blight resistance in hexaploid wheat-Lophopyrium genetic line and tagging of the alien chromatin by PCR markers. 2003. Annual Meetings for American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Denver, CO, USA, p206.

31) X. Shen, L. Kong, and H. Ohm. 2003. Pyramiding FHB resistance derived from wheatgrass with QTLs from wheat. Annual Meetings for American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Denver, CO, USA, p206.

32) L. Kong, Y. Dong, R.- C. Wang. 1997. Analysis in Aegilops squrrosa L. Plant & Animal Genome V Conference, San Diego, California, USA. P66.

33) L. Kong, F. Guo, and H. Wang. 1993. Cytoembryological studies on low fertility in hybrids between octoploid Agrotriticum and Triticum aestivum L. Proc. Int. Wheat Genet. Symp. 8th Beijing, P. R. China. Vol. 1: 261-265.

1988－1993，普通遺傳學（本科生），山東農業大學農學系。

1996－1999，普通遺傳學（本科生），細胞遺傳學（研究生），山東農業大學農學系。

1999－2000，細胞遺傳學（研究生），生物技術在農業上的應用（研究生），山東農業大學農學系。

2001－2007，分子標記和分子標記輔助育種（AGRY480）（高年級本科生和研究生），美國普度大學農學系。

學術團體會員

1．The Scientific Research Society Sigma Xi.

2．American Society of Agronomy (ASA).

3．Crop Science Society of American (CSSA).