周恩民
周恩民
周恩民,1993年獲加拿大明尼多巴大學醫學院免疫學博士學位。1992年至2006年在加拿大農業部動物疾病研究所、加拿大國家動物外來病研究中心及美國依阿華州立大學獸醫學院擔任高級研究員、教授和免疫室主任。在國外20餘年科研教學中主持和參加加拿大及美國的有關動物病毒、疫苗和單克隆抗體等領域科研項目20餘個。發表SCI論文50餘篇。
2021年9月23日2時36分在楊凌家中逝世,享年64歲。
2010年7月--:
2007年7月至2010年6月:
山東省“泰山學者海外特聘專家”;
山東農業大學動物科技學院、動物醫學院副院長;
2006年5月至2007年7月:
“龍江學者”特聘教授:黑龍江八一農墾大學動物科技學院
2000年6月至2006年4月:
副教授、主任:美國衣阿華州立大學獸醫學院獸醫診斷和動物生產醫學系
2002年12月至2005年12月:
1998年9月至2000年6月:
副教授:加拿大明尼多巴大學農學院動物科學系,兼職
1992年4月至2000年5月:
主任、高級研究員:加拿大國家食品檢驗檢疫總局國家外來病中心免疫診斷實驗室
1995年1月1998年12月:
客座副教授:加拿大渥太華大學微生物免疫學系
1985年9月1988年8月:
訪問學者,博士后:美國德可薩思州聖安東尼奧西南生物醫學研究所病毒免疫學系
1978年9月至1985年8月:
助教:包頭醫學院微生物教研室
首次發現了藍耳病病毒自身抗獨特型抗體的存在。該抗體模擬藍耳病病毒的表面抗原,識別抗該表面抗原的抗體。相繼製備了單克隆抗獨特型抗體,並由此鑒定出了一個新型的藍耳病病毒受體,發現了該受體的阻斷抑製劑。獲得了國家發明專利。
戊型肝炎病毒研究
首次證明禽HEV(美國分離株)的 ORF2 蛋白免疫后能夠產生中和抗體並可以防止HEV的感染。ORF2 蛋白的抗原表位可以作為有效的免疫診斷抗原,用來鑒別人和禽HEV抗原決定簇。研究表明禽與人、豬HEV具有某些相同的抗原決定簇。最近首次在中國患有肝脾腫大綜合征的雞病料中分離到禽HEV,已完成全基因組鑒定。
先後在 J. Immunol. (免疫學雜誌)、J. Virol.(病毒學雜誌)、J. Gen. Virol.(普通病毒學雜誌)J. Virol. Methods(病毒方法學雜誌)等刊物上發表67篇論文,在國際會議上發表論文70餘篇。
開設課程:本科生《獸醫免疫學》及全英文《獸醫免疫學》;
研究生《獸醫免疫學研究進展》、《獸醫學專題》。
論文: (*為通訊作者):(共 68篇)
1.Qin Zhao, En-Min Zhou*, Shi-Wei Dong, Hong-Kai Qiu, Lu Zhang, Shou-Bin Hu, Fei-Fei Zhao, Shi-Jin Jiang, and Ya-Ni Sun. Genetic identification and sequence analysis of avian hepatitis E virus from chickens in China. Accepted for publication at Emerging and Infectious Diseases. 2010:.
3.Shao-Ning Liu, Xing-Xiao Zhang, Jin-Feng Zou, Zhi-Jing Xie, Yan-Li Zhu, Qin Zhao, En-min Zhou, Shi-Jin Jiang. Development of an indirect ELISA for the detection of duck circovirus infection in duck flocks. Vet Microbiol. 2010, online March 17
4.Zhao Q, Sun YN, Zhang XX, Kong YB, Xie ZJ, Zhu YL, Zhou EM, Jiang SJ. Evaluation of two experimental infection models for Avibacterium paragallinarum. Vet Microbiol. 2010; 141(1-2):68-72.
5.李曉靜,周恩民*,趙欽,肖一紅,馬利宏。一株豬繁殖與呼吸綜合征病毒的分離及ORF5基因序列與國內外毒株的分析和比較。西南農業學報(Southwest China Journal of Agricultural Sciences).2009; 22(4):1103-1109.
6.張紅霞,荊勝濤,周恩民*,孫培明,董施偉,李艷菊。人血清中抗禽戊型肝炎病毒抗體的檢測與鑒定。中國衛生檢驗雜誌 (Chin J Health Lab Technol). 2009; 19(8):1741-1744.
7.周恩民,荊勝濤,彭軍,孫培明,張璐,董施偉。禽戊性肝炎病毒人獸共感染的可能性研究。中國家禽. 2009; 31(1):5-9.
9.周恩民*,荊勝濤,張璐,董施偉,彭軍,孫培明。禽戊型肝炎研究進展。山東畜牧獸醫。2008; 11:1-3.
11.冉旭華,孟凡,聞曉波,單玉波,周恩民。豬細小病毒NS1蛋白主要抗原表位區的原核表達。生物技術(Biaotechnology)。2008; 18(2):18-20.
12.Zhou E-M*, Xiao YH, Shi YF, Li XJ, Ma LH, Jing ST. Generation of internal image monoclonal anti-idiotypic antibodies against idiotypic antibodies to GP5 antigen of porcine reproductive and respiratory syndrome virus. J Virol Methods. 2008; 149:300-308. Impact factor: 2.097.
13.Zhou E-M*, Guo H, Huang FF, Sun ZF, Meng XJ. Identification of two neutralization epitopes on the capsid protein of avian hepatitis E virus (avian HEV). J Gen Virol. 2008; 89:500-508. Impact factor: 3.300. (Cited 1)
14.Guo H, Zhou EM, Sun ZF, Meng XJ. Immunodominant epitopes mapped by synthetic peptides on the capsid protein of avian hepatitis E virus are non-protective. Viral Immunol. 2008; 21(1):61-7. (Cited 1)
15.Boyer TC, Ward MP, Wallace RL, Zhou E-M, Singer RS. Exploratory spatial data analysis of regional seroprevalence of antibodies against epizootic hemorrhagic disease virus in cattle from Illinois and Indiana. Ame J Vet Res. 2008; 69(10):1286-1293.
16.Guo H, Zhou E-M*, Sun ZF, Meng XJ. Protection of chickens against avian hepatitis E virus (avian HEV) infection by immunization with recombinant avian HEV capsid protein. Vaccine. 2007; 25:2892-2899. Impact factor: 2.822.
17.Guo H, Zhou E-M*, Sun ZF, Meng XJ. Egg whites from eggs of chickens experimentally infected with avian hepatitis E virus contain infectious virus but evidence of complete vertical transmission is lacking. J Gen Virol. 2006; 88:1532-1537. Impact factor: 3.300.
18.Guo H, Zhou E-M,* Sun ZF, Meng XJ, Halbur PG. Identification of B-cell epitopes in the capsid protein of avian hepatitis E virus (avian HEV) that are common to human and swine HEVs or unique to avian HEV. J Gen Virol. 2006; 87(1):217-223. Impact factor: 3.300. (Cited 1)
19.Trampel DW, Zhou E-M, Yoon KJ, Koehler KJ. Detection of antibodies in serum and egg yolk following infection of chickens with an H6N2 avian influenza virus. J Vet Diagn Invest. 2006; 18(5):437-442. Impact factor: 1.609. (Cited 1)
20.Opriessnig T, McKeown NE, Zhou E-M, Meng XJ, Halbur PG. Genetic and experimental comparison of porcine circovirus type 2 (PCV2) isolates from cases with and without PCV2-associated lesions provides evidence for differences in virulence. J Gen Virol. 2006; 87(Pt 10):2923-2932. Impact factor: 3.300. (Cited 3)
21.Ameri-Mahabadi M and Zhou E-M. Idiotype and anti-idiotype: a review. Comp Clin Pathol. 2006; 14:171-178.
22.Ameri-Mahabadi M, Zhou E-M*, Hsu W. A Western blot immunoassay as a confirmatory test for the presence of anti-mycoplasma hyopneumoniae antibodies in swine serum. J Vet Diagn Invest. 2006; 18(2):198-201. Impact factor: 1.609.
23.Ameri-Mahabadi M, Zhou E-M,* Hsu W. Comparison of two swine mycoplasma hyopneumoniae enzyme-linked Immunosorbent assays for detection of antibodies from vaccinated pigs and field serum samples. J Vet Diagn Invest. 2005; 17: 61-64. Impact factor: 1.609. (Cited 2)
24.Clavijo A, Zhou E-M, Hole K, Galic B, Kitching P. Development and use of a biotinylated 3ABC recombinant protein in a solid-phase competitive ELISA for the detection of antibodies against foot-and-mouth disease virus. J Virol Methods. 2004; 15:217-227. Impact factor: 1.938. (Cited 1)
25.Zhou E-M*, Clavijo A, Jiang Z, Ameri-Mahabadi M, Zimmerman JJ. Induction of auto-anti-idiotypic antibodies specific for antibodies to matrix and envelope glycoprotein from pigs experimentally infected with porcine reproductive and respiratory syndrome virus. Vet Immunol Immunopathol. 2004; 101:49-59. Impact factor: 1.659.
26.Skibbe D, Zhou E-M*, Janke BH. Comparison of a commercial ELISA with hemagglutination inhibition assay for serdiagnosis of swine influenza virus (H1N1) infection. J Vet Diagn Invest. 2004; 16:86-89. Impact factor: 1.609.
27.Zhou J-Y, Chen Q-X, Ye J-X, Zhu J-X, Zheng X-J, Shang S-B, Chen T-F, Mu A-X, Zhou E-M. Serological investigation of porcine circovirus 2 infection. Chinese J Vet Med. 24:1-3. (豬二型圓環病毒的血清學調查。<<中國獸醫學雜誌>>,2004年第24期,1-3頁)。
28.Zhou J-Y, Wu J-X, Cheng L-Q, Zheng X-J, Gong H, Shang S-B and Zhou E-M*. Expression of immunogenic S1 glycoprotein of infectious bronchitis virus in transgenic potatoes. J Virol. 2003; 77(16):9090-9093. Impact factor: 5.241. (Cited 2)
29.Jiang ZJ, Zhou E-M*, Ameri-Mahabadi M, Zimmerman JJ and Platt KB. Identification and characterization of auto-anti-idiotypic antibodies specific for antibodies against porcine reproductive and respiratory syndrome virus envelope glycoprotein. Vet Immunol Immunopathol. 2003; 92:125-135. Impact factor: 1.659.
30.Clavijo A, Tresnan DB, Jolie R, Zhou E-M. Comparison of embryonated chicken eggs with MDCK cell culture for the isolation of swine influenza virus. Can J Vet Res. 2002; 66:117-121. Impact factor: 1.114. (Cited 5)
31.Zhou E-M*, Ridd D, Riva J, Fernando L, Clavijo A. Development and evaluation of an IgM-capture ELISA for detection of recent infection with bluetongue virus in cattle. J Virol Methods. 2001; 91:175-182. Impact factor: 1.938.
32.Zhou E-M*, Riva J, Clavijo A. Development of an immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay for detection of equine and swine IgM antibodies to vesicular stomatitis virus. Clin Diagn Lab Immunol. 2001; 8:475-481. Impact factor: 1.654. (Cited 1)
33.Clavijo A, Lin M, Riva J, Zhou E-M. Application of competitive enzyme-linked immunosorbent assay for the serological diagnosis of classical swine fever virus infection. J Vet Diagn Invest. 2001; 13:357-360. Impact factor: 1.609. (Cited 1)
34.Clavijo A, Lin M, Riva J, Mallory M, Lin F, Zhou E-M. Development of a competitive ELISA using a truncated E2 recombinant protein as antigen for detection of antibodies to classical swine fever virus. Res Vet Sci. 2001; 70:1-7. Impact factor: 1.011.
35.Clavijo A, Riva J, Copps J, Robinson Y, Zhou E-M. Assessment of the pathogenicity of an emu-origin influenza A H5 virus in ostriches (Struthio camelus). Avian Pathol. 2001; 30:83-89. Impact factor: 1.514.
36.Pasick J, Handel, K, Zhou E-M, Clavijo A, Coates J, Robinson Y, and Lincoln B. Incursion of epizootic hemorrhagic disease into the Okanogan Valley, British Columbia in 1999. Can Vet J. 2001; 42:207-209. Impact factor: 0.742.
37.Clavijo A, Munroe F, Zhou E-M, Booth TF, Roblesky K. Incursion of bluetongue into the Okanogan Valley, British Columbia. Can Vet J. 2000; 41:312-314. Impact factor: 0.742.
38.Heckert A, McIsaac M., Chan M, Zhou E-M. Experimental infection of emus (Dromaius novaehollandiae) with avian influenza virus of various virulence: clinical signs, virus shedding and serology. Avian Pathol. 1999; 23:13-16. Impact factor: 1.514.
39.Zhou E-M*, Afshar A. Characterization of monoclonal antibodies to epizootic hemorrhagic disease of deer virus (EHDV) and bluetongue virus by immunization of mice with EHDV recombinant VP7 antigen. Res Vet Sci. 1999; 66:247-252. Impact factor: 1.011.
40. Zhou E-M. Anti-idiotype technique: an alternative approach for immunodiagnosis of bluetongue. Vet Immunol Immunopathol. 1999; 72:237-242. Impact factor: 1.659.
41. Wright P, Zhou E-M. Developments in international standardization. Vet Immunol Immunopathol. 1999; 72:243-248. Impact factor: 1.659.
42. Zhou E-M*, Chan M, Heckert RA, Riva J, Cantin M-F. Evaluation of a competitive ELISA for detection of antibodies against avian influenza virus nucleoprotein. Avian Dis. 1998; 42:517-522. Impact factor: 1.151.
43. Clavijo A, Zhou E-M, Vydelingum S, Heckert R. Development and evaluation of a novel antigen capture assay for the detection of classical swine fever virus antigens. Vet Microbiol. 1998; 60:155-168. Impact factor: 1.727. (Cited 5)
44. Zhou E-M*, Chan M, McIsaac M, Heckert R. Evaluation of antibody responses of emus (Dromaius novaehollandiae) to avian influenza virus infection. Avian Dis. 1998; 42:757-761. Impact factor: 1.151.
45. Xu G, Wilson W, Mecham J, Murphy K, Zhou E-M, Tabachnick W. VP7: an attachment protein of bluetongue virus for cellular receptors in Culicoides variipennes. J Gen Virol. 1997; 78:1617-1623. Impact factor: 3.300. (Cited 5)
46. Prud'homme I., Zhou E-M, Traykova M, Trotter H, Chan M, Afshar A, Harding MJ. Production of a baculovirus-derived gp50 protein and utilization in a competitive enzyme-linked immunosorbent assay for the serodiagnosis of pseudorabies virus. Can J Vet Res. 1997; 61:286-291. Impact factor: 1.114.
47. Zhou E-M*, Chan MKH. Immunoblot assay as a confirmatory test of competitive ELISA for serodiagnosis of bluetongue. J Vet Diagn Invest. 1996; 8(3):365-367. Impact factor: 1.609.
48. Lin M, Zhou E-M*. Induction of antibodies to the bluetongue virus core polypeptide VP7 in sheep by internal image rabbit anti-idiotypic antibodies. Viral Immunol. 1996; 9(1):35-43. Impact factor: 1.625. (Cited 1)
49. Lin M, Zhou E-M*. Evidence that two distinct populations of rabbit anti-idiotypic antibodies induced by three monoclonal antibodies specific for bluetongue virus core protein VP7. Microbiol Immunol. 1996; 40(4):295-302.
50. Lin M, Zhou E-M*. Biological mimicry of the bluetongue virus core protein VP7 by rabbit anti-idiotype. Microbiol Immunol. 1996; 40(6):435-441.
51. Zhou E-M, Kisil FT. Regulation of antibody formation to Ryegrass pollen allergen Lol pIV by an internal image anti-idiotypic monoclonal antibody. Immunol. 1995; 84:343-349. Impact factor: 2.729.
52. Zhou E-M*, Huang W. Anti-idiotypic antibody as a potential serodiagnostic reagent detecting bluetongue virus infection. J Clin Microbiol. 1995; 33(4):850-854. Impact factor: 3.565.
53. Zhou E-M*, Afshar A. Comparison of Freund's adjuvant and TiterMaxTM in inducing anti-idiotype to idiotypic antibodies against pseudorabies virus antigens. Vet Immunol Immunopathol. 1995; 48:113-122. Impact factor: 1.659.
54. Lin M, Zhou E-M*.Internal image anti-idiotypic antibody detects sheep antibodies to the bluetongue virus core protein VP7. Immunotechnol. 1995; 1:151-155. Impact factor: 2.598.
55. Zhou E-M*, Kisil FT. Antibody responses to allergen Lol pIV are suppressed following adoptive transfer of B lymphocytes from the internal image anti-idiotypic antibody treated mice. Cell Immunol. 1995; 165:77-83. Impact factor: 1.988.
56.Zhou E-M*, Afshar A, Heckert RA, Nielsen K. Anti-idiotypic antibodies generated by sequential immunization detect the shared idiotype on antibodies to pseudorabies virus antigens. J Virol Methods. 1994; 48:301-313. Impact factor: 1.938. (Cited 1)
57.Zhou E-M, Dzuba-Fisher JMM, Rector ES, Sehon AH, Kisil FT. A mouse monoclonal anti-idiotypic antibody detects a common idiotope on human, mouse, and rabbit antibodies to allergen Lol pIV. Scand J Immunol. 1991; 34:307-316. Impact factor: 1.782. (Cited 2)
58.Zhou E-M, Lohman KL, Kennedy RC. Administration of noninternal image monoclonal anti-idiotypic antibodies induces idiotype-restricted responses specific for human immunodeficiency virus envelope glycoprotein epitopes. Virol. 1990; 174:9-17. Impact factor: 3.363. (Cited 5)
59.Zhou E-M, Kennedy RC. Strategies for developing vaccines against acquired immunodeficiency syndrome. Biochem Biophysica Acta Cancer Res Suppl. 1989; 986:301-313. Impact factor: 2.935. (Cited 1)
60.Zhou E-M*, Wang D-R, Kennedy RC. Anti-idiotypic antibodies as potential candidates of vaccines. Chinese J Immunol. 1988; 2:70-73.
61.Zhou E-M, Dreesman GR, Kennedy RC. Anti-idiotypic antibodies: A new generation of vaccines against infectious agents. Microbiol Sci. 1987; 4:36-40. (Cited 3)
62.Kennedy RC, Zhou E-M, Lanford RE, Chanh TC, Bona CA. Possible role of anti-idiotypic antibodies in the induction of tumor immunity. J Clin Invest. 1987; 80: 1217-1224. Impact factor: 14.051. (Cited 6)
63.Zhou E-M, Chanh TC, Dreesman GR, Kanda P, Kennedy RC. Immune response to human immunodeficiency virus: In vivo administration of anti-idiotype induces an anti-gp160 response specific for a synthetic peptide. J Immunol. 1987; 139:2950-2956. Impact factor: 7.014. (Cited 6)
64.Ren Q, Jin Z-C, Zhou E-M. Influence of uniform intensity magnetic field on the antibody forming cells in mice. J Inner Mongolia Med. 1981; 1(3):146-149.
65.王承寶,張沖(學),周恩民,趙欽,梁超(學),黨露(學),馬玉萍(學),高繼明(外),李瓊毅(學),黃柏成(學),孔寧(外);Complete Genome Sequence of a Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Variant;2012-08;Journal of Virology;SCI(2012)(5.402)
書章節:
66.Lowe DB, Shearer MH, Jumper CA, Zhou E-M, Kennedy. 2008. Plasmid DNA as prophylactic and therapeutic vaccines for cancer and infectious diseases. In Plasmids: Current Research and Future Trends (Georg Lipps ed.), Horizon Scientific Press.
67.Zhou E-M*, Lin M. 1997. Anti-idiotype to bluetongue virus VP7 antigen – potential candidate of diagnostic reagent and vaccine. in Idiotypes in Medicine - Infections, Autoimmunity and Cancer (Y. Shoenfeld, R.C. Kennedy and T. Ferrone ed.), Elsevier Science, The Netherlands.
68.Dreesman GR, Henkel RD, Zhou E-M, Kennedy RC. 1988. Properties of a protective anti-idiotype vaccine for viral hepatitis B. In Vaccine: New concepts and Developments (H. Kohler and P.T. LoVerde, eds.) Longman Group Ltd., UK.
2.豬iPS 細胞系的建立及其基因打靶。國家高技術研究發展計劃(863 計劃)專題課題:高效基因連續敲除技術子課題。2009,01-2011,12。
3. 高致病性藍耳病風險分析關鍵技術與應用研究。國家行業科技計劃項目:動物衛生風險分析關鍵技術與應用研究子課題。2009,01-2011,12.
4. 藍耳病病毒新型受體突變基因的體細胞克隆。國家重大專項課題。2009,07-2010,12。
5. 新型PRRSV細胞受體鑒定及其與病毒相互作用機制的研究。國家自然基金。2009,01-2011,12.
6. 新型PRRSV淋巴細胞受體與PRRSV免疫反應的關係及抗獨特性抗體對疫苗免疫的調控。獸醫生物技術國家重點實驗室開放課題。2009,01-2010,12。
7. 豬重大疫病防治標準化技術體系應用與示範。山東省自主創新成果轉化重大專項項目。2008,01-2010,12.
2009年:獲“第二屆百名華僑華人專業人士‘傑出創業獎’”
2009年:泰安市優秀科技創新團隊“動物生物技術”帶頭人
2008年:EPIZONE Workshop: Research in swine viral diseases “Building Bridge”分會主席
2008年:中國農業部動物疫病風險分析評估委員會委員
2007年:第十五屆世界禽病大會(北京)分會主席
2006年:獲黑龍江省大慶市第二十一屆自然科學技術學術成果獎(禽戊型肝炎病抗原決定簇鑒定及疫苗研究)
1998年:獲加拿大美洲農業總署獎勵赴巴拉圭推廣藍舌病和流行性出血熱酶標免疫診斷試劑以促進國際貿易
1997年:獲加拿大美洲農業總署獎勵赴阿根廷推廣藍舌病酶標免疫診斷試劑以促進國際貿易
1997年:第5屆國際免疫學大會免疫學診斷分會主席
1994年:國際獸用疫苗大會新型疫苗分會主席
1992年:獲加拿大明尼多巴省醫學研究優秀研究獎
1990年:獲加拿大明尼多巴大學醫學研究基金最佳優秀博士研究獎
1987年:任《Viral Immunology》雜誌特約審稿人
1984年:獲包頭醫學院優秀教師獎
主要學術兼職:
國家自然科學基金委員會第十三界生命科學部獸醫學評審組成員,2010-
中國農業科學院學位論文評閱專家,2010-
中國農業部全國動物衛生風險評估專家委員會委員,2008-
中國畜牧獸醫學會獸醫公共衛生學分會常務理事,2008-
中國畜牧獸醫學會高級會員,2008-
中國免疫學會高級會員,2007-
科學雜誌編委及特邀審稿人:
Viral Immunology
Journal of Virological Methods
Journal of Diary Research
American Journal of Veterinary Research
Transboundary and Emerging Diseases (Formerly ‘Journal of Veterinary Medicine, Series A’)
陝西省動物疫情應急防治隊專家組副組長、
國務院學位委員會第七屆學科評議組(獸醫學)成員、
第九屆農業部科學技術委員會委員、
教育部高等學校動物醫學專業教學指導委員會副主任委員、
第四屆國家轉基因生物安全委員會委員、
農業部基因克隆與轉基因技術執行專家組成員、
農業部全國動物衛生風險評估專家委員會委員、
中國免疫學會獸醫免疫分會第五屆委員會副主任委員、
中國畜牧獸醫學會家畜傳染病學分會常務理事、
中國畜牧獸醫學會獸醫公共衛生學分會常務理事